High Density Culture of the Human Hepatoma Cell Line HepG2: Long-Term Culture for In Vitro Toxicology

2006 ◽  
pp. 721-724
Author(s):  
A. Handa-Corrigan ◽  
R.M Traynor ◽  
I. Adamopoulos ◽  
J. Salway
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
High Density Culture ◽  
Long Term Culture ◽  
Hepatoma Cell Line Hepg2

The novel hydroxynaphthoquinone 566C80 inhibits the development of liver stages of Plasmodium berghei cultured in vitro

Parasitology ◽  
1993 ◽  
Vol 106 (1) ◽  
pp. 1-6 ◽  
Author(s):  
C. S. Davies ◽  
M. Pudney ◽  
J. C. Nicholas ◽  
R. E. Sinden
Keyword(s):  
Cell Line ◽  
Plasmodium Berghei ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
The Novel ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Highly Active ◽  
Hepatoma Cell Line Hepg2

SUMMARYThe causal prophylactic activity of the novel hydroxynaphthoquinone, S66C80, was assessed against the exo-erythrocytic (EE) stages of Plasmodium berghei cultured in the human hepatoma cell line, HepG2. 566C80 was found to be highly active as an inhibitor of EE development and was more active than the established causal prophylactic pyrimethamine. A 566C80 concentration of 1·85 × 10−9 M, added 3 h after sporozoite invasion, reduced the numbers of EE forms visible at 48 h by 50%, while the equivalent concentration of pyrimethamine was 1·95 × 10−8 M.

scholarly journals Synthetic peptides from the circumsporozoite proteins of Plasmodium falciparum and Plasmodium knowlesi recognize the human hepatoma cell line HepG2-A16 in vitro.

1986 ◽  
Vol 164 (6) ◽  
pp. 1915-1922 ◽  
Author(s):  
S B Aley ◽  
M D Bates ◽  
J P Tam ◽  
M R Hollingdale
Keyword(s):  
Cell Line ◽  
Plasmodium Knowlesi ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Repeat Region ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Hepatoma Cell Line Hepg2

Several lines of evidence have emphasized the importance of the malaria circumsporozoite (CS) protein as a factor in sporozoite invasion of the hepatocyte; however, the specific mechanism of cell recognition and invasion has not been explained. In this study we present evidence that a highly conserved region of the CS protein immediately adjacent to the repeat region, the N1 region, specifically recognizes receptors on the human hepatoma cell line HepG2-A16 under conditions where invasion by sporozoites can occur. Peptides consisting of sequences from the repeat region or of the more extensive N2 region showed no such specific association. Antibody against the N1 peptide could inhibit sporozoite invasion in vitro. Covalent coupling of radiolabeled N1 peptide to HepG2-A16 cells identified two hepatic cell proteins to be closely associated with the peptide. We suggest that these proteins could act as receptors or mediators, via the N1 region of the CS protein, for the P. falciparum sporozoite in the process of invasion of the hepatocyte.

scholarly journals 3D-Bioreactor culture of human hepatoma cell line HepG2 as a promising tool for in vitro substance testing

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P61 ◽  
Author(s):  
Christiane Goepfert ◽  
Wibke Scheurer ◽  
Susanne Rohn ◽  
Britta Rathjen ◽  
Stefanie Meyer ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Bioreactor Culture ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Promising Tool ◽  
Hepatoma Cell Line Hepg2
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