Immortalization Strategies for Epithelial Cells in Primary Culture

2007 ◽  
pp. 616-639 ◽  
Author(s):  
David de Semir Frappart ◽  
Rosalie Maurisse ◽  
Esther H. Vock ◽  
Dieter C. Gruenert
Keyword(s):  
Epithelial Cells ◽  
Primary Culture

Primary culture of canine prostate epithelial cells

1982 ◽  
Vol 7 (2) ◽  
pp. 51-54 ◽  
Author(s):  
Louis Terracio ◽  
William H. J. Douglas
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Prostate Epithelial Cells

Putrescine stimulates growth of human bronchial epithelial cells in primary culture

In Vitro ◽  
1980 ◽  
Vol 16 (5) ◽  
pp. 399-406 ◽  
Author(s):  
Gary D. Stoner ◽  
Curtis C. Harris ◽  
Gwendolyn A. Myers ◽  
Benjamin F. Trump ◽  
Robert D. Connor
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals Immunohistochemical characterisation of epithelial cells of rodent harderian glands in primary culture

1999 ◽  
Vol 195 (4) ◽  
pp. 523-530 ◽  
Author(s):  
YASMINA DJERIDANE ◽  
VALERIE SIMONNEAUX ◽  
PAUL KLOSEN ◽  
BERTHE VIVIEN-ROELS ◽  
PAUL PEVET
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Harderian Glands ◽  
Immunohistochemical Characterisation

Contact behaviour during the reassociation of dissociated epithelial cells in primary culture

1987 ◽  
Vol 88 (4) ◽  
pp. 521-526
Author(s):  
R.M. Brown ◽  
C.A. Middleton
Keyword(s):  
Epithelial Cells ◽  
Chick Embryo ◽  
Primary Culture ◽  
Cell Cultures ◽  
Corneal Epithelium ◽  
Time Lapse ◽  
Cell Types ◽  
Epidermal Cells ◽  
Isolated Cells ◽  
Contact Behaviour

The behaviour in culture of dissociated epithelial cells from chick embryo pigmented retina epithelium (PRE), corneal epithelium (CE) and epidermis has been studied using time-lapse cinematography. The analysis concentrated on the contact behaviour of 60 previously isolated cells of each type during a 24 h period starting 3.5 h after the cells were plated out. During the period analysed the number of isolated cells in cultures of all three types gradually decreased as they became incorporated into islands and sheets of cells. However, there were significant differences in behaviour between the cell types during the establishment of these sheets and islands. In PRE cell cultures, islands of cells developed because, throughout the period of analysis, collisions involving previously isolated cells almost invariably resulted in the development of a stable contact. Once having established contact with another cell these cells rarely broke away again to become reisolated. In contrast the contacts formed between colliding CE and epidermal cells were, at least initially, much less stable and cells of both these types were frequently seen to break away and become reisolated after colliding with other cells. Sheets and islands of cells eventually developed in these cultures because the frequency with which isolated cells become reisolated decreased with increasing time in culture. The possible reasons underlying the different behaviour of PRE cells, when compared with that of CE and epidermal cells, are discussed. It is suggested that the decreasing tendency of isolated CE and epidermal cells to become reisolated may be related to the formation of desmosomes.

Long-term primary culture of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver

1995 ◽  
Vol 31 (5) ◽  
pp. 367-378 ◽  
Author(s):  
Gary K. Ostrander ◽  
James B. Blair ◽  
Beverly A. Stark ◽  
Garry M. Marley ◽  
Wesley D. Bales ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Epithelial Cells ◽  
Oncorhynchus Mykiss ◽  
Primary Culture ◽  
Rainbow Trout Oncorhynchus Mykiss

Anion-dependent Mg2+ influx and a role for a vacuolar H+-ATPase in sheep ruminal epithelial cells

2003 ◽  
Vol 285 (1) ◽  
pp. G45-G53 ◽  
Author(s):  
Monika Schweigel ◽  
Holger Martens
Keyword(s):  
Epithelial Cells ◽  
Atpase Activity ◽  
Primary Culture ◽  
Intracellular Ph ◽  
Uptake Rate ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Fluorescence Techniques ◽  
Transport Inhibitors ◽  
Stimulation Of

The K+-insensitive component of Mg2+ influx in primary culture of ruminal epithelial cells (REC) was examined by means of fluorescence techniques. The effects of extracellular anions, ruminal fermentation products, and transport inhibitors on the intracellular free Mg2+ concentration ([Mg2+]i), Mg2+ uptake, and intracellular pH were determined. Under control conditions (HEPES-buffered high-NaCl medium), the [Mg2+]i of REC increased from 0.56 ± 0.14 to 0.76 ± 0.06 mM, corresponding to a Mg2+ uptake rate of 15 μM/min. Exposure to butyrate did not affect Mg2+ uptake, but it was stimulated (by 84 ± 19%) in the presence of [Formula: see text]. In contrast, Mg2+ uptake was strongly diminished if REC were suspended in [Formula: see text]-buffered high-KCl medium (22.3 ± 4 μM/min) rather than in HEPES-buffered KCl medium (37.5 ± 6 μM/min). After switching from high- to low-Cl– solution, [Mg2+]i was reduced from 0.64 ± 0.09 to 0.32 ± 0.16 mM and the [Formula: see text]-stimulated Mg2+ uptake was completely inhibited. Bumetanide and furosemide blocked the rate of Mg2+ uptake by 64 and 40%, respectively. Specific blockers of vacuolar H+-ATPase reduced the [Mg2+]i (36%) and Mg2+ influx (38%) into REC. We interpret this data to mean that the K+-insensitive Mg2+ influx into REC is mediated by a cotransport of Mg2+ and Cl– and is energized by an H+-ATPase. The stimulation of Mg2+ transport by ruminal fermentation products may result from a modulation of the H+-ATPase activity.

Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells

1987 ◽  
Vol 7 (5) ◽  
pp. 2031-2034
Author(s):  
D E Brash ◽  
R R Reddel ◽  
M Quanrud ◽  
K Yang ◽  
M P Farrell ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Bronchial Epithelial Cells ◽  
Large T Antigen ◽  
Bronchial Epithelial ◽  
Antigen Gene ◽  
Strontium Phosphate

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

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