Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells
Keyword(s):
Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).
1987 ◽
Vol 7
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pp. 2031-2034
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2010 ◽
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pp. 137-143
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1989 ◽
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pp. 10075-10079
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1989 ◽
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1991 ◽
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pp. 50-56
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2009 ◽
Vol 11
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pp. 1646-1654
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