Defined Culture Media for Human Embryonic Stem Cells

2007 ◽  
pp. 1-16 ◽  
Author(s):  
Tenneille Ludwig ◽  
James A. Thomson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Culture Media ◽  
Embryonic Stem ◽  
Defined Culture

scholarly journals Chemically defined sequential culture media for TH+ cell derivation from human embryonic stem cells

2008 ◽  
Vol 14 (11) ◽  
pp. 619-625 ◽  
Author(s):  
T. Song ◽  
G. Chen ◽  
Y. Wang ◽  
G. Mao ◽  
Y. Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Culture Media ◽  
Embryonic Stem ◽  
Th Cell

Caspase Inhibitor Z-VAD-FMK Enhances the Freeze-Thaw Survival Rate of Human Embryonic Stem Cells

2007 ◽  
Vol 27 (4-5) ◽  
pp. 257-264 ◽  
Author(s):  
Boon Chin Heng ◽  
Marie Veronique Clement ◽  
Tong Cao
Keyword(s):  
Stem Cells ◽  
Survival Rate ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Culture Media ◽  
Embryonic Stem ◽  
Immunocytochemical Staining ◽  
Irreversible Inhibitor ◽  
Slow Cooling ◽  
Spontaneous Differentiation

Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence, this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes, Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 100 mM Z-VAD-FMK was supplemented into either the freezing solution, the post-thaw culture media or both. Intact and adherent hESC colonies were cryopreserved so as to enable subsequent quantitation of the post-thaw cell survival rate through the MTT assay, which can only be performed with adherent cells. Exposure to 100 mM Z-VAD-FMK in the freezing solution alone did not significantly enhance the post-thaw survival rate (10.2% vs. 9.9%, p > 0.05). However, when 100 mM Z-VAD-FMK was added to the post-thaw culture media, there was a significant enhancement in the survival rate from 9.9% to 14.4% (p < 0.05), which was further increased to 18.7% when Z-VAD-FMK was also added to the freezing solution as well (p < 0.01). Spontaneous differentiation of hESC after cryopreservation was assessed by morphological observations under bright-field microscopy, and by immunocytochemical staining for the pluripotency markers SSEA-3 and TRA-1-81. The results demonstrated that exposure to Z-VAD-FMK did not significantly enhance the spontaneous differentiation of hESC within post-thaw culture.

scholarly journals Defined culture conditions of human embryonic stem cells

2006 ◽  
Vol 103 (15) ◽  
pp. 5688-5693 ◽  
Author(s):  
J. Lu ◽  
R. Hou ◽  
C. J. Booth ◽  
S.-H. Yang ◽  
M. Snyder
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Culture Conditions ◽  
Defined Culture

scholarly journals Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells

2010 ◽  
Vol 46 (3-4) ◽  
pp. 247-258 ◽  
Author(s):  
Veronika Akopian ◽  
◽  
Peter W. Andrews ◽  
Stephen Beil ◽  
Nissim Benvenisty ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Feeder Cell ◽  
Culture Systems ◽  
Defined Culture ◽  
Free Propagation
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