Hansenula polymorpha (Pichia angusta): Biology and Applications

2009 ◽  
pp. 47-64 ◽  
Author(s):  
Gotthard Kunze ◽  
Hyun Ah Kang ◽  
Gerd Gellissen
Keyword(s):  
Hansenula Polymorpha ◽  
Pichia Angusta

Construction of flavocytochrome b 2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta)

Microbiology ◽  
2008 ◽  
Vol 77 (2) ◽  
pp. 181-185 ◽  
Author(s):  
K. V. Dmitruk ◽  
O. V. Smutok ◽  
M. V. Gonchar ◽  
A. A. Sibirnyi
Keyword(s):  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Pichia Angusta ◽  
Flavocytochrome B

Hansenula polymorpha (Pichia angusta)

1996 ◽  
pp. 293-311 ◽  
Author(s):  
Hans Hansen ◽  
Cornelis P. Hollenberg
Keyword(s):  
Hansenula Polymorpha ◽  
Pichia Angusta

scholarly journals Rad51 Protein from the Thermotolerant Yeast Pichia angusta as a Typical but Thermodependent Member of the Rad51 Family

2004 ◽  
Vol 3 (6) ◽  
pp. 1567-1573 ◽  
Author(s):  
Valery I. Shalguev ◽  
Yury V. Kil ◽  
Ludmila V. Yurchenko ◽  
Eugene A. Namsaraev ◽  
Vladislav A. Lanzov
Keyword(s):  
Atp Hydrolysis ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Product Formation ◽  
Rad51 Protein ◽  
Recombination Repair ◽  
Reaction Characteristics ◽  
Pichia Angusta ◽  
Dna Specificity ◽  
Interaction Position

ABSTRACT The Rad51 protein from the methylotrophic yeast Pichia angusta (Rad51Pa) of the taxonomic complex Hansenula polymorpha is a homolog of the RecA-RadA-Rad51 protein superfamily, which promotes homologous recombination and recombination repair in prokaryotes and eukaryotes. We cloned the RAD51 gene from the cDNA library of the thermotolerant P. angusta strain BKM Y1397. Induction of this gene in a rad51-deficient Saccharomyces cerevisiae strain partially complemented the survival rate after ionizing radiation. Purified Rad51Pa protein exhibited properties typical of the superfamily, including the stoichiometry of binding to single-stranded DNA (ssDNA) (one protomer of Rad51Pa per 3 nucleotides) and DNA specificity for ssDNA-dependent ATP hydrolysis [poly(dC) > poly(dT) > φX174 ssDNA > poly(dA) > double-stranded M13 DNA]. An inefficient ATPase and very low cooperativity for ATP interaction position Rad51Pa closer to Rad51 than to RecA. Judging by thermoinactivation, Rad51Pa alone was 20-fold more thermostable at 37°C than its S. cerevisiae homolog (Rad51Sc). Moreover, it maintained ssDNA-dependent ATPase and DNA transferase activities up to 52 to 54°C, whereas Rad51Sc was completely inactive at 47°C. A quick nucleation and an efficient final-product formation in the strand exchange reaction promoted by Rad51Pa occurred only at temperatures above 42°C. These reaction characteristics suggest that Rad51Pa is dependent on high temperatures for activity.

Use of the yeast Hansenula polymorpha (Pichia angusta) to remove contaminating sugars from ethyl β-d-fructofuranoside produced during sucrose ethanolysis catalysed by invertase

1999 ◽  
Vol 87 (1) ◽  
pp. 82-86 ◽  
Author(s):  
Anna M. van der Heijden ◽  
Pim van Hoek ◽  
Janko Kaliterna ◽  
Johannes P. van Dijken ◽  
Fred van Rantwijk ◽  
...  
Keyword(s):  
Hansenula Polymorpha ◽  
Pichia Angusta

scholarly journals Chromosome Polymorphisms among Strains of Hansenula polymorpha (syn. Pichia angusta)

1993 ◽  
Vol 59 (3) ◽  
pp. 939-941 ◽  
Author(s):  
Laura Marri ◽  
Gian Maria Rossolini ◽  
Giuseppe Satta
Keyword(s):  
Hansenula Polymorpha ◽  
Pichia Angusta

Hansenula polymorpha: Biology and Applications. Edited by G. Gellissen. Wiley-VCH, Weinham, 2002

Yeast ◽  
2003 ◽  
Vol 20 (15) ◽  
pp. 1307-1308 ◽  
Author(s):  
Peter Sudbery
Keyword(s):  
Hansenula Polymorpha

Investigations on the possible role of the endoplasmic reticulum in the development of peroxisomes in the yeast Hansenula polymorpha

1985 ◽  
Vol 51 (5-6) ◽  
pp. 572-572
Author(s):  
M. Veenhuis ◽  
A. C. Douma ◽  
P. G. Bruinenberg ◽  
W. Harder
Keyword(s):  
Endoplasmic Reticulum ◽  
Hansenula Polymorpha
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