Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence In Situ 5′ Nuclease Assay

2000 ◽  
pp. 123-134
Author(s):  
Bruce K. Patterson
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Reverse Transcriptase ◽  
Nuclease Assay

Direct in situ reverse transcriptase-linked polymerase chain reaction with biotinylated primers for the detection of hepatitis C virus RNA in liver biopsies

1999 ◽  
Vol 12 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Dominique Bettinger ◽  
Christiane Mougin ◽  
Brigitte Fouqué ◽  
Bernadette Kantelip ◽  
Jean Philippe Miguet ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Reverse Transcriptase ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Virus Rna ◽  
Liver Biopsies ◽  
Polymerase Chain

scholarly journals Detection and Localization by In Situ Molecular Biology Techniques and Immunohistochemistry of Hepatitis C Virus in Livers of Chronically Infected Patients

1998 ◽  
Vol 46 (5) ◽  
pp. 653-660 ◽  
Author(s):  
Francine M. Walker ◽  
Marie-Christine Dazza ◽  
Marie-Christine Dauge ◽  
Olivier Boucher ◽  
Christophe Bedel ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Interferon Α ◽  
Rt Pcr ◽  
Biopsy Specimens ◽  
Bile Ductule ◽  
Liver Biopsies ◽  
Cytoplasmic Signals ◽  
Detection And Localization

Hepatitis C virus (HCV) detection in the livers of chronically infected patients remains a debatable issue. We used immunohistochemistry, in situ hybridization (ISH) alone or after microwave heating with FITC-labeled probes, RT-PCR with unlabeled primers followed by ISH (RT-PCR-ISH), and in situ RT-PCR with FITC-labeled primers (in situ RT-PCRd) to localize the virus in 38 liver biopsy specimens from 21 chronically infected HCV patients treated with interferon-α (IFN-α). Biopsies were taken at the beginning and end of IFN-α treatment and 1 year later. Results were compared with that of HCV-PCR in serum. RT-PCR-ISH and in situ RT-PCRd showed HCV signal in all liver biopsies even in responders with seronegative HCV PCR. This signal was intranuclear, diffuse, or peripheral, in hepatocytes, bile ductule cells, and lymphocytes. Cytoplasmic signals were occasionally observed. Whereas the percentage of labeled hepatocytes remained constant, the number of labeled lymphoid follicles decreased after INF-α therapy. Immunohistochemistry resulted in the same pattern of positivity but it was weaker and inconstant. This study indicates the persistency of HCV latency in IFN-α responders 1 year after IFN-α treatment cessation, a finding that certainly deserves confirmation.

Detection of hepatitis C virus RNA in the liver by in situ hybridization

2008 ◽  
Vol 13 (4) ◽  
pp. 203-208 ◽  
Author(s):  
Yujiro Tanaka ◽  
Nobuyuki Enomoto ◽  
Shigeru Kojima ◽  
Liang Tang ◽  
Mieko Goto ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis C Virus Rna ◽  
Virus Rna
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