Expression of Chimeric Human Aspartic Proteinases

1998 ◽  
pp. 139-146
Author(s):  
John M. Chirgwin ◽  
Suzanne Schultz ◽  
Deepali Sachdev
Keyword(s):  
Aspartic Proteinases

Peptide Inhibitors of Aspartic Proteinases with Hydroxyethylene Isostere Replacement of Peptide Bond. II. Preparation of Pseudotetrapeptides Derived from Diastereoisomeric 5-Amino-2-benzyl-4-hydroxy-6-phenylhexanoic Acids

1998 ◽  
Vol 63 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Jaroslav Litera ◽  
Jan Weber ◽  
Ivana Křížová ◽  
Iva Pichová ◽  
Jan Konvalinka ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Peptide Bond ◽  
Peptide Inhibitors ◽  
Aspartic Proteinases ◽  
Hiv 1

Twelve pseudotetrapeptides, Boc-NHCH(CH2Ph)CH(OH)CH2CH(CH2Ph) CO-Xaa-Phe-NH2 9-11, were prepared by [(benzotriazol-1-yl)oxy]tris(dimethylamino)phosphonium hexafluorophosphate-mediated couplings of diastereoisomeric O-silylated (2R or 2S,4R or 4S,5S)-2-benzyl-5-(tert-butoxycarbonyl)amino-4-hydroxy-6-phenylhexanoic acids 1 with dipeptides H-Xaa-Phe-NH2 (Xaa = Gln, Glu(OBzl) or Ile) 3-5, followed by O-deprotection. Pseudotetrapeptides 9-11 were tested for inhibition of aspartic proteinases secreted by Candida albicans and C. tropicalis. The level of inhibition of both yeast proteinases was very low, contrasting with the nanomolar IC50 values obtained for inhibition of HIV-1 proteinase.

scholarly journals Purification of two fungal aspartic proteinases using fast protein liquid chromatography.

1990 ◽  
Vol 54 (6) ◽  
pp. 1563-1565 ◽  
Author(s):  
Eric D. BROWN ◽  
Melodie G. WYNNE ◽  
Anthony J. CLARKE ◽  
Rickey Y. YADA
Keyword(s):  
Liquid Chromatography ◽  
Fast Protein Liquid Chromatography ◽  
Aspartic Proteinases

Aspartic Proteinases from Parasites

1996 ◽  
pp. 247-250 ◽  
Author(s):  
John Kay ◽  
Lorraine Tyas ◽  
Michelle J. Humphreys ◽  
Jeff Hill ◽  
Ben M. Dunn ◽  
...  
Keyword(s):  
Aspartic Proteinases
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