Construction of Cloning Vectors from the IncW Plasmid pSa and their use in Analysis of Crown Gall Tumor Formation

1983 ◽  
pp. 111-123 ◽  
Author(s):  
R. C. Tait ◽  
T. J. Close ◽  
M. Hagiya ◽  
R. C. Lundquist ◽  
C. I. Kado
Keyword(s):  
Crown Gall ◽  
Tumor Formation ◽  
Cloning Vectors ◽  
Crown Gall Tumor

Inhibition of crown-gall tumor formation on potato discs by cyclic-AMP and prostaglandins E1 and E2

1977 ◽  
Vol 18 (2) ◽  
pp. 469-472 ◽  
Author(s):  
Shelley Favus ◽  
Olga Gonzalez ◽  
Pamela Bowman ◽  
Alan Galsky
Keyword(s):  
Cyclic Amp ◽  
Crown Gall ◽  
Tumor Formation ◽  
Crown Gall Tumor ◽  
Prostaglandins E1 And E2

A QUANTITATIVE BIOASSAY FOR CROWN-GALL TUMOR FORMATION

1955 ◽  
Vol 42 (8) ◽  
pp. 709-712 ◽  
Author(s):  
Richard M. Klein ◽  
Ida L. Tenenbaum
Keyword(s):  
Crown Gall ◽  
Tumor Formation ◽  
Crown Gall Tumor ◽  
Quantitative Bioassay

scholarly journals Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation

2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
E. Szegedi ◽  
A. Oberschall ◽  
S. Bottka ◽  
R. Oláh ◽  
D. Tinland
Keyword(s):  
Crown Gall ◽  
Plant Transformation ◽  
Northern Blot Analysis ◽  
Tumor Formation ◽  
Pcr Analysis ◽  
Wild Type ◽  
Crown Gall Tumor ◽  
Transformation Vector ◽  
Plant Transformation Vector ◽  
Foreign Dna

The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.  

scholarly journals Cumulative Cytotoxicity Assay of the Aqueous and Ethanolic Extracts of the Selected Medicinal Plants using Crown Gall Tumor Disc Bioassay

2020 ◽  
Author(s):  
Sandeep Waghulde ◽  
Tushar Baviskar ◽  
Praful Patil ◽  
Shweta Singh ◽  
Nilesh Gorde ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crown Gall ◽  
Cytotoxicity Assay ◽  
Crown Gall Tumor ◽  
Ethanolic Extracts
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