Initial Interactions Between Plant Cells and Agrobacterium Tumefaciens in Crown Gall Tumor Formation

1988 ◽  
pp. 99-126
Author(s):  
Gerard A. Cangelosi ◽  
Eugene W. Nester
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Plant Cells ◽  
Tumor Formation ◽  
Crown Gall Tumor

Inhibition of crown-gall tumor formation on potato discs by cyclic-AMP and prostaglandins E1 and E2

1977 ◽  
Vol 18 (2) ◽  
pp. 469-472 ◽  
Author(s):  
Shelley Favus ◽  
Olga Gonzalez ◽  
Pamela Bowman ◽  
Alan Galsky
Keyword(s):  
Cyclic Amp ◽  
Crown Gall ◽  
Tumor Formation ◽  
Crown Gall Tumor ◽  
Prostaglandins E1 And E2

DEOXYRIBONUCLEIC ACID DEPENDENT RIBONUCLEIC ACID SYNTHESIS IN THE CROWN-GALL TUMOR-INDUCING ORGANISM AGROBACTERIUM TUMEFACIENS

1963 ◽  
Vol 41 (7) ◽  
pp. 1503-1518 ◽  
Author(s):  
R. M. Hochster ◽  
V. M. Chang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Messenger Rna ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Nucleoside Triphosphate ◽  
Satisfactory Explanation ◽  
Crown Gall Tumor ◽  
Other Information ◽  
Hydrolysis Of

The enzyme RNA polymerase has been partially purified from cell-free extracts of the crown-gall tumor-inducing organism Agrobacterium tumefaciens. The four triphosphates ATP, CTP, GTP, and UTP, manganese (or magnesium) ions, and DNA are all required for activity. DNA acts as a template in the formation of the new RNA molecule the base composition of which exactly mimics that of the particular DNA used. The dependence of the reaction on time, pH, and on the concentrations of nucleoside triphosphate, DNA, and protein has been worked out. The exact requirements of the entire system are delineated, the effect of physical alteration of the DNA used (heating, cooling, sonic oscillation) has been examined and a new observation made on the stimulation of DNA action by 1-minute sonic pretreatment.Actinomycin D is shown to inhibit the reaction completely at 2.8 × 10−5 M while atabrine, a new inhibitor, requires a concentration of 3.3 × 10−3 M under the conditions specified. Hydrolysis of the reaction product by means of a variety of procedures and other information obtained show that the reaction product is, indeed, RNA.The data reported herein are regarded as providing a satisfactory explanation for the mechanism of biosynthesis of at least one type of RNA (presumably "messenger" RNA) in A. tumefaciens.

ON THE NUCLEOTIDE SPECIFICITY OF THE POLYNUCLEOTIDE PHOSPHORYLASE OF THE CROWN-GALL TUMOR-INDUCING ORGANISM AGROBACTERIUM TUMEFACIENS

1961 ◽  
Vol 39 (11) ◽  
pp. 1695-1704 ◽  
Author(s):  
A. Vardanis ◽  
R. M. Hochster
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Rna Synthesis ◽  
The Other ◽  
Moderate Activity ◽  
Polynucleotide Phosphorylase ◽  
Crown Gall Tumor ◽  
Nucleotide Specificity

An investigation of the nucleotide specificity of the polynucleotide phosphorylase of Agrobacterium tumefaciens was undertaken, using the measurable increase in viscosity as an index of activity. It was found that ADP and CDP were polymerized readily and at approximately equal rates. The enzyme exhibited more moderate activity with UDP and was completely inactive with GDP. The ineffectiveness of the enzyme with mixtures of all four ribonucleoside diphosphates was traced to the ability of GDP to act as an inhibitor in the polymerization of the other diphosphates. Evidence is presented to show that the inhibition of poly A synthesis effected by GDP is competitive. On the basis of the results obtained it is concluded that the polynucleotide phosphorylase is not likely to be responsible for RNA synthesis in A. tumefaciens.

Untersuchungen an Crown-Gall

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Tumor Induction ◽  
Host System ◽  
Induced Tumors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.

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