scholarly journals Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation

2001 ◽  
Vol 7 (3-4) ◽  
Author(s):  
E. Szegedi ◽  
A. Oberschall ◽  
S. Bottka ◽  
R. Oláh ◽  
D. Tinland
Keyword(s):  
Crown Gall ◽  
Plant Transformation ◽  
Northern Blot Analysis ◽  
Tumor Formation ◽  
Pcr Analysis ◽  
Wild Type ◽  
Crown Gall Tumor ◽  
Transformation Vector ◽  
Plant Transformation Vector ◽  
Foreign Dna

The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.  

Inhibition of crown-gall tumor formation on potato discs by cyclic-AMP and prostaglandins E1 and E2

1977 ◽  
Vol 18 (2) ◽  
pp. 469-472 ◽  
Author(s):  
Shelley Favus ◽  
Olga Gonzalez ◽  
Pamela Bowman ◽  
Alan Galsky
Keyword(s):  
Cyclic Amp ◽  
Crown Gall ◽  
Tumor Formation ◽  
Crown Gall Tumor ◽  
Prostaglandins E1 And E2

scholarly journals Gene Stacking for Fungal Resistance in Plant Transformation Vector

2017 ◽  
Vol 14 (3) ◽  
pp. 1211-1219
Author(s):  
Sonia Sharma ◽  
Gurtej Singh ◽  
Sadiq Pasha Shaik ◽  
Gagandeep Singh ◽  
Sumangala Bhat ◽  
...  
Keyword(s):  
Plant Transformation ◽  
Fruit Production ◽  
Fungal Diseases ◽  
Fungal Resistance ◽  
Hindiii Site ◽  
Transformation Vector ◽  
Fungal Disease Resistance ◽  
Genes Encoding ◽  
Production Form ◽  
Plant Transformation Vector

ABSTRACT: Fungal diseases like early blight, late blight, fusarium wilt cause 30-40 per cent loss in fruit production. Form past decade many transgenic plants had been developed using genes encoding chitinases and glucanases with the objective of imparting fungal disease resistance. Since the genes encoding chitinase and glucanase act synergistically. The study was performed to construct plant transformation vector pRAGS carrying both ech42 and bgn under single T-DNA region. Initially, HindIII site at 5' end of earlier cloned bgn (T. harzianum) was removed using primers during reamplification of the gene. The amplicons were cloned into pTZ57R/T containing T overhangs at Eco321 site and transferred to E. coli DH5a and further to plant transformation vector pBI121 which was named as pRA121. In order to clone another gene (ech42) into pRA121, expression cassette from iHP vector was transferred to pRA121 and named as pRAG121. Further in order to gain XhoI site for cloning ech42 gene into pRAG121, ech42 (pSUM1) was cloned into pYES2/CT, named as pSAG1, ech42 from pSAG1 cloned with KpnI and XhoI in pRAG121 and named as pRAGS121. The vector constructed in the present study can be used to transform important crop plants to have enhanced resistance to fungal diseases.

Construction of a new type of multi-gene plant transformation vector and genetic transformation of tobacco

2017 ◽  
Vol 61 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Y. Dong ◽  
Y. C. Ren ◽  
M. S. Yang ◽  
J. Zhang ◽  
T. Qiu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Plant Transformation ◽  
Transformation Vector ◽  
New Type ◽  
Plant Transformation Vector

scholarly journals No Evidence of Unexpected Transgenic Insertions in T1190 – A Transgenic Apple Used in Rapid Cycle Breeding – Following Whole Genome Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Andrea Patocchi ◽  
Jens Keilwagen ◽  
Thomas Berner ◽  
Stefanie Wenzel ◽  
Giovanni A. L. Broggini ◽  
...  
Keyword(s):  
Chromosome 4 ◽  
Wild Type ◽  
Breeding Programs ◽  
Transformation Vector ◽  
Step Procedure ◽  
Dna Insertion ◽  
Apple Plant ◽  
Plant Transformation Vector ◽  
Reference Genomes ◽  
Transgenic Apple

Rapid cycle breeding uses transgenic early flowering plants as crossbreed parents to facilitate the shortening of breeding programs for perennial crops with long-lasting juvenility. Rapid cycle breeding in apple was established using the transgenic genotype T1190 expressing the BpMADS4 gene of silver birch. In this study, the genomes of T1190 and its non-transgenic wild-type PinS (F1-offspring of ‘Pinova’ and ‘Idared’) were sequenced by Illumina short-read sequencing in two separate experiments resulting in a mean sequencing depth of 182× for T1190 and 167× for PinS. The sequencing revealed 8,450 reads, which contain sequences of ≥20 bp identical to the plant transformation vector. These reads were assembled into 125 contigs, which were examined to see whether they contained transgenic insertions or if they are not using a five-step procedure. The sequence of one contig represents the known T-DNA insertion on chromosome 4 of T1190. The sequences of the remaining contigs were either equally present in T1190 and PinS, their part with sequence identity to the vector was equally present in apple reference genomes, or they seem to result from endophytic contaminations rather than from additional transgenic insertions. Therefore, we conclude that the transgenic apple plant T1190 contains only one transgenic insertion, located on chromosome 4, and shows no further partial insertions of the transformation vector.Accession Numbers: JQ974028.1.

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