Methods for Measuring Functional Properties of Sarcoplasmic Reticulum and Myofibrils in Small Samples of Myocardium

1984 ◽  
pp. 49-61 ◽  
Author(s):  
Edward D. Pagani ◽  
R. John Solaro
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Functional Properties ◽  
Small Samples

Alterations in the functional properties of skinned fibers from denervated rabbit skeletal muscle

1990 ◽  
Vol 259 (3) ◽  
pp. C503-C506 ◽  
Author(s):  
M. M. Trachez ◽  
R. T. Sudo ◽  
G. Suarez-Kurtz
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Functional Properties ◽  
Dose Response ◽  
Response Curve ◽  
Extensor Digitorum Longus ◽  
Isometric Tension ◽  
Skinned Fibers ◽  
Spontaneous Release

Isometric tension was recorded in vitro from chemically skinned fibers obtained from normal and 14-day-denervated extensor digitorum longus muscles of the rabbit. Denervation potentiated the tensions elicited by pCa 6.0 but did not modify the pCa value (5.6) required for maximum tension. Ca2+ transport across the membranes of the sarcoplasmic reticulum (SR) was markedly affected by denervation. Thus the rate of ATP-dependent net Ca2+ uptake increased significantly, and the spontaneous release ("leakage") of the Ca2+ stored in the SR was significantly reduced in denervated fibers. These effects lead to increased accumulation of Ca2+ in the lumen of the SR. The dose-response curve for the halothane-induced contractures of Ca2(+)-loaded skinned fibers was displaced to the left after denervation. Thus 0.7 mM halothane, a concentration that elicited no tension in 10 control fibers, induced contractures in the 10 denervated fibers tested. The potentiation of the halothane-induced tensions is attributed mainly to the larger stores of Ca2+ in the SR of denervated fibers. The possibility that denervation may also affect the interaction of halothane with the SR membranes is discussed.

Functional Properties of Human Embryonic Stem Cell-Derived Cardiomyocytes: Intracellular Ca2+Handling and the Role of Sarcoplasmic Reticulum in the Contraction

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 236-245 ◽  
Author(s):  
Katya Dolnikov ◽  
Mark Shilkrut ◽  
Naama Zeevi-Levin ◽  
Sharon Gerecht-Nir ◽  
Michal Amit ◽  
...  
Keyword(s):  
Stem Cell ◽  
Sarcoplasmic Reticulum ◽  
Embryonic Stem Cell ◽  
Functional Properties ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

scholarly journals Functional Properties of Sarcoplasmic Reticulum Ca2+-ATPase after Proteolytic Cleavage at Leu119-Lys120, Close to the A-domain

2003 ◽  
Vol 279 (10) ◽  
pp. 9156-9166 ◽  
Author(s):  
Guillaume Lenoir ◽  
Martin Picard ◽  
Carole Gauron ◽  
Cédric Montigny ◽  
Pierre Le Maréchal ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Functional Properties ◽  
Proteolytic Cleavage

scholarly journals Sarcoplasmic Reticulum Structure and Functional Properties that Promote Long-Lasting Calcium Sparks

2016 ◽  
Vol 110 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Daisuke Sato ◽  
Thomas R. Shannon ◽  
Donald M. Bers
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Functional Properties ◽  
Calcium Sparks

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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