Principles of Multi-Enzyme Purification by Affinity Chromatography

1978 ◽  
pp. 441-442 ◽  
Author(s):  
C.-Y. Lee ◽  
A. Leigh-Brown ◽  
C. Langley ◽  
B. Pegoraro ◽  
J. Lopez-Barea ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification

scholarly journals Biospecific-elution chromatography with ‘imphilytes’ as stationary phases

1977 ◽  
Vol 161 (2) ◽  
pp. 233-237 ◽  
Author(s):  
R J Yon
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification ◽  
Stationary Phases ◽  
Chromatographic Behaviour ◽  
Presence And Absence ◽  
Elution Chromatography

Six out of seven enzymes tested (four of them nicotinamide nucleotide-dependent dehydrogenases) showed differences in chromatographic behaviour in the presence and absence of their biospecific ligands, when chromatographed on immobilized amphipathic ampholytes (‘imphilytes’) as stationary phases. Some enzymes were adsorbed more tightly, others less tightly, in the presence of ligands. These results have implications for enzyme purification in general, and for some types of affinity chromatography in particular.

scholarly journals Easy assembly of ligands for glycosidase affinity chromatography

1990 ◽  
Vol 270 (2) ◽  
pp. 539-540 ◽  
Author(s):  
R C Bernotas ◽  
B Ganem
Keyword(s):  
Amino Acids ◽  
Affinity Chromatography ◽  
Enzyme Purification ◽  
Reductive Amination ◽  
Water Soluble ◽  
High Yield ◽  
Glycosidase Inhibitors ◽  
Neutral Ph ◽  
Sepharose 4B ◽  
Derivatives Of

An improved, high-yield synthesis of the corresponding N-carboxypentyl derivatives of three iminoalditol glycosidase inhibitors has been developed for affinity chromatography enzyme purification. Reductive amination of 1-deoxynojirimycin (or its D-manno or D-galacto analogues) with methyl 5-formylvalerate and NaBH3CN at neutral pH afforted an aminoester which upon hydrolysis with aqueous 5% HCl gave the desired aminoacid in 97% overall yield. These amino acids could then be covalently attached using water-soluble carbodi-imide to 6-aminohexyl Sepharose 4B.

Biomimetic dyes as affinity chromatography tools in enzyme purification

2000 ◽  
Vol 891 (1) ◽  
pp. 33-44 ◽  
Author(s):  
Y.D Clonis ◽  
N.E Labrou ◽  
V.Ph Kotsira ◽  
C Mazitsos ◽  
S Melissis ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification

scholarly journals Selective enzyme purification by affinity chromatography.

1968 ◽  
Vol 61 (2) ◽  
pp. 636-643 ◽  
Author(s):  
P. Cuatrecasas ◽  
M. Wilchek ◽  
C. B. Anfinsen
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification

scholarly journals An application of affinity chromatography to enzyme purification

1978 ◽  
Vol 22 (2) ◽  
pp. 123-128
Author(s):  
Kazuo Murakami ◽  
Fumiaki Suzuki ◽  
Hideyuki Kobayashi
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification

Overexpression of (His) 6 -tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography

2012 ◽  
Vol 81 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Andriy E. Zakalskiy ◽  
Oksana M. Zakalska ◽  
Yuriy A. Rzhepetskyy ◽  
Natalia Potocka ◽  
Oleh V. Stasyk ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Affinity Chromatography ◽  
Enzyme Purification ◽  
Metal Affinity ◽  
Arginase I
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