Molecular Regulation of Blood-Brain Barrier GLUT1 Glucose Transporter

1995 ◽  
pp. 81-88 ◽  
Author(s):  
William M. Pardridge
Keyword(s):  
Blood Brain Barrier ◽  
Glucose Transporter ◽  
Molecular Regulation ◽  
Brain Barrier ◽  
Blood Brain ◽  
Glut1 Glucose Transporter

Upregulation of blood-brain barrier GLUT1 glucose transporter protein and mRNA in experimental chronic hypoglycemia

Diabetes ◽  
1995 ◽  
Vol 44 (12) ◽  
pp. 1399-1404 ◽  
Author(s):  
A. K. Kumagai ◽  
Y. S. Kang ◽  
R. J. Boado ◽  
W. M. Pardridge
Keyword(s):  
Blood Brain Barrier ◽  
Glucose Transporter ◽  
Brain Barrier ◽  
Transporter Protein ◽  
Blood Brain ◽  
Glut1 Glucose Transporter

Upregulation of Blood-Brain Barrier GLUT1 Glucose Transporter Protein and mRNA in Experimental Chronic Hypoglycemia

Diabetes ◽  
1995 ◽  
Vol 44 (12) ◽  
pp. 1399-1404 ◽  
Author(s):  
A. K. Kumagai ◽  
Y.-S. Kang ◽  
R. J. Boado ◽  
W. M. Pardridge
Keyword(s):  
Blood Brain Barrier ◽  
Glucose Transporter ◽  
Brain Barrier ◽  
Transporter Protein ◽  
Blood Brain ◽  
Glut1 Glucose Transporter

scholarly journals Role of the GLUT1 Glucose Transporter in Postnatal CNS Angiogenesis and Blood-Brain Barrier Integrity

2020 ◽  
Vol 127 (4) ◽  
pp. 466-482 ◽  
Author(s):  
Koen Veys ◽  
Zheng Fan ◽  
Moheb Ghobrial ◽  
Ann Bouché ◽  
Melissa García-Caballero ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Glucose Transporter ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity ◽  
Glut1 Glucose Transporter

scholarly journals Interictal Seizure Resections Show Two Configurations of Endothelial Glut1 Glucose Transporter in the Human Blood–Brain Barrier

1998 ◽  
Vol 18 (1) ◽  
pp. 26-42 ◽  
Author(s):  
Eain M. Cornford ◽  
Shigeyo Hyman ◽  
Marcia E. Cornford ◽  
Elliot M. Landaw ◽  
Antonio V. Delgado-Escueta
Keyword(s):  
Endothelial Cells ◽  
Brain Tissue ◽  
Blood Brain Barrier ◽  
Glucose Transporter ◽  
Brain Barrier ◽  
Immunogold Electron Microscopy ◽  
Partial Seizures ◽  
Complex Partial Seizures ◽  
Blood Brain ◽  
Glut1 Glucose Transporter

Immunogold electron microscopy was used to analyze and quantify the Glut1 glucose transporter in brain tissue from five patients undergoing surgery for treatment of seizures. Samples were prepared from two different regions of each resection: (1) the most actively spiking epileptogenic site, and (2) the least actively spiking region, as indicated by intraoperative EEG monitoring. Two configurations of endothelial cell Glut1 were observed. About one half of the capillary profiles examined displayed abundant Glut1 immunoreactivity on both luminal and abluminal endothelial membranes. In the remainder of the profiles, reduced Glut1 labeling was seen, but adjacent erythrocyte membranes remained highly Glut1 immunoreactive, suggesting that reduced endothelial Glut1 reactivity was not attributable to method artifacts. Immunogold studies using antisera to human glial fibrillary acidic protein and human serum albumin demonstrated increased quantities of these two epitopes in the extravascular regions in which more EEG spiking activity had been demonstrated. These observations were consistent with the hypotheses that capillary integrity was more compromised, and gliosis was quantitatively increased, in the more actively spiking region of the resection. Altered glucose transporter activity in the blood–brain barrier was characterized by a bimodal Glut1 distribution in which the smaller (type B) endothelial cells displayed low Glut1 immunoreactivity, whereas adjacent (and even contiguous) larger (type A) endothelial cells showed 5- to 10-fold greater expression of membrane Glut1 transporter protein. Because this transporter facilitates glucose entry to the brain, small pericapillary volumes of brain tissue may have quite different concentrations of glucose. We hypothesize that in complex partial seizures and other forms of brain insult, an alteration of blood–brain barrier Glut1 glucose transporter activity is indicated by the appearance of these two subpopulations of endothelial cells. In comparison with previous studies of human brain capillaries in hemangioblastoma and brain injury, endothelial Glut1 density was apparently reduced (interictally) in affected temporal lobes of patients with complex partial seizures.

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