scholarly journals Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies

2014 ◽  
pp. 63-95 ◽  
Author(s):  
Arlie J. Rinaldi ◽  
Krishna C. Suddala ◽  
Nils G. Walter
Keyword(s):  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies

scholarly journals Single-molecule fluorescence studies of RNA: A decade’s progress

2009 ◽  
Vol 476 (1-3) ◽  
pp. 1-10 ◽  
Author(s):  
Krishanthi S. Karunatilaka ◽  
David Rueda
Keyword(s):  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies

scholarly journals Single-molecule fluorescence studies on cotranscriptional G-quadruplex formation coupled with R-loop formation

2020 ◽  
Vol 48 (16) ◽  
pp. 9195-9203
Author(s):  
Gunhyoung Lim ◽  
Sungchul Hohng
Keyword(s):  
Gene Regulation ◽  
Single Molecule ◽  
Genome Instability ◽  
Feedback Mechanism ◽  
Rnase H ◽  
Formation Mechanisms ◽  
Loop Formation ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies ◽  
G Quadruplex

Abstract G-quadruplex (GQ) is formed at various regions of DNA, including telomeres of chromosomes and regulatory regions of oncogenes. Since GQ is important in both gene regulation and genome instability, the biological and medical implications of this abnormal DNA structure have been intensively studied. Its formation mechanisms, however, are not clearly understood yet. We report single-molecule fluorescence experiments to monitor the cotranscriptional GQ formation coupled with R-loop formation using T7 RNA polymerase. The GQ is formed very rarely per single-round transcription. R-loop formation precedes and facilitates GQ formation. Once formed, some GQs are extremely stable, resistant even to RNase H treatment, and accumulate in multiple-round transcription conditions. On the other hand, GQ existing in the non-template strand promotes the R-loop formation in the next rounds of transcription. Our study clearly shows the existence of a positive feedback mechanism of GQ and R-loop formations, which may possibly contribute to gene regulation and genome instability.

scholarly journals Maximizing Photon Counts and Time Resolution for Single Molecule Fluorescence Studies in Micro Fluidic Mixing System

2011 ◽  
Vol 100 (3) ◽  
pp. 137a
Author(s):  
Denis Doerr ◽  
Ralf Kuenemuth ◽  
Shawn H. Pfeil ◽  
Everett A. Lipman ◽  
Claus A.M. Seidel
Keyword(s):  
Single Molecule ◽  
Time Resolution ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies
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