NAA-Induced Direct Organogenesis from Female Immature Inflorescence Explants of Date Palm

2017 ◽  
pp. 17-25
Author(s):  
Hussam S. M. Khierallah ◽  
Saleh M. Bader ◽  
Makki A. Al-Khafaji
Keyword(s):  
Date Palm ◽  
Direct Organogenesis ◽  
Immature Inflorescence ◽  
Inflorescence Explants

scholarly journals Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Rania A. Taha ◽  
Mai A. Allam ◽  
S. A. M. Hassan ◽  
Basem M. M. Bakr ◽  
Mona M. Hassan
Keyword(s):  
Growth Regulators ◽  
Date Palm ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Immature Inflorescence ◽  
Plant Materials ◽  
Indole Butyric Acid ◽  
Promising Tool ◽  
Inflorescence Explants

Abstract Background Inflorescence explants of date palm proved to be a promising tool for micropropagation of elite cultivars or rare males and females as organogenesis and somatic embryogenesis could be achieved. These plant materials are abundantly available every year and can be used as cheap and potent explants. Nevertheless, many difficulties could be faced in this protocol according to selection of the spathe size and age, media components, growth regulators, etc. The aim of this study was to determine the influence of various cytokinins on direct organs induction of three date palm cultivars (Selmi, Barhee, and Medjool) from immature inflorescence. An additional objective of this study was to investigate the effect of cytokinins and auxins on growth and development of Medjool cultivar. Results Various combinations of cytokinins were investigated on three date palm inflorescences as N6-(2-isopentenyl) adenine (2iP), kinetin, benzyleadenine (BA), and thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-yl urea) (TDZ). TDZ alone or in combination with BA proved to be superior for direct organogenesis in all three cultivars so that another combination of TDZ with BA was conducted. Results showed that moderate concentration of BA, with TDZ, gave superior response. Medjool cultivar response surpassed other two cultivars that made the possibility to conduct some growth regulators treatments on its multiplication and regeneration. TDZ at 0.5 + BA at 1.0 mg/l without activated charcoal seemed to enhance multiplication rate. Medium containing 0.5 mg/l of both naphthaleneacetic acid and indole butyric acid in addition to 1.0 mg/l indole acetic acid appeared to be more suitable for rooting stage of Medjool shootlets. Conclusion In this study, we created an innovation sequence of growth regulators included in nutrient media for date palm direct organogenesis from inflorescence. Organogenesis has been accelerated from immature inflorescence explants and developed to healthy plantlets which acclimatized in greenhouse.

scholarly journals Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements

CORD ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 11
Author(s):  
Anitha Karun
Keyword(s):  
Tissue Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Zygotic Embryo ◽  
Shoot Meristem ◽  
Direct Organogenesis ◽  
Zygotic Embryos ◽  
Immature Inflorescence ◽  
High Yielding Varieties

Coconut is one of the principal crops of India cultivated in over 35 districts mainly in the southern states. The productivity of the crop is declining in many of the traditionally cultivated regions owing to ageing plantations as well as biotic and abiotic stresses. These plantations are to be replanted with high yielding varieties/hybrids for which adequate quantity of quality planting material is not available. Even though tissue culture research was initiated in many laboratories in the country, the work was eventually phased out in most of the laboratories for want of a repeatable protocol.  At ICAR-CPCRI, coconut tissue culture programs have been continuing for the past three decades. The attempts made include experimentation with different explants viz., immature inflorescence, plumular tissues, mature palm shoot meristem, ovary and anthers and different culture media supplemented with varying levels and types of hormones. Some of the successful protocols developed at the Institute include coconut zygotic embryo culture for collection and exchange of germplasm, cryopreservation and retrieval of zygotic embryos and pollen and plantlet regeneration from plumular tissues. Even though ICAR-CPCRI has succeeded in obtaining plantlets via direct organogenesis from inflorescence explants, the absence of friable calli formation from explants, the low rate of somatic embryo formation, large number of cultures turning to abnormal shoot development, non conversion of somatic embryos into plantlets, and formation of abnormal somatic embryos remain the major bottlenecks. Gene expression studies are being currently undertaken to decipher the molecular basis of in vitro recalcitrance.

scholarly journals The Role of Silver Thiosulphate and Glutamine on Direct Organogenesis of Two Date Palm (Phoenix dactylifera L.) Cultivars

2009 ◽  
Vol 3 (1) ◽  
pp. 37-45
Author(s):  
Saleh M. Bader ◽  
Hussam S. M. Khierallah
Keyword(s):  
Date Palm ◽  
Culture Media ◽  
Phoenix Dactylifera ◽  
Direct Organogenesis ◽  
Adventitious Bud ◽  
Ms Medium ◽  
Silver Thiosulphate ◽  
Phoenix Dactylifera L ◽  
Modified Ms Medium

This study was conducted to examine the effect of silver thiosulphate (STS) and glutamine at various concentrations on direct organogenesis and shoots multiplication of date palm (Phoenix dactylifera L.) Barhi and Maktom cvs. Shoot tip quarters were cultured in vitro on modified MS medium supplemented with 2.0 mg/1 2ip, 1.0 mg/1 BA, 1.0 mg/1 NAA and 1.0 mg/1 NOA with various concentrations of STS (30, 60, 90 and 120 µM) and glutamine at (0.35, 0.70, 1.4 and 2.1 mM). The same concentrations were used in multiplication stage with medium supplemented with 4.0 mg/1 2ip, 2 mg/1 BA, 1.0 mg/1 NAA and 1.0 mg/1 NOA. Results indicated that the addition of STS at 90 µM and glutamine at 0.7 mM gave the best result for adventitious bud formation after 16 weeks (8.1 and 9.4 buds per explant for Barhi and Maktom respectively). Subculturing of these buds on agitated liquid MS medium with 90 µM STS and 1.4 mM glutamine gave the highest average of shoots number (12.5 and 14.7 shoots for Barhi and Maktom respectively). Maktom cultivar produced more buds and shoots than Barhi irrespective of the level of STS and glutamine. Shoots were rooted and successfully transferred to soil. These results confirm the importance of supplementing plant culture media of date palm cvs. Barhi and Maktom with both silver thiosulphate and glutamine in order to optimize and improve the clonal propagation protocol for these two cultivars.

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