Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry

2019 ◽  
pp. 105-119
Author(s):  
Antonella Testa ◽  
Valentina Palma ◽  
Clarice Patrono
Keyword(s):  
Dicentric Chromosome ◽  
Biological Dosimetry ◽  
Cbmn Assay

Biological Dosimetry by the Triage Dicentric Chromosome Assay: Potential Implications for Treatment of Acute Radiation Syndrome in Radiological Mass Casualties

2011 ◽  
Vol 175 (3) ◽  
pp. 397-404 ◽  
Author(s):  
Horst Romm ◽  
Ruth C. Wilkins ◽  
C. Norman Coleman ◽  
Patricia K. Lillis-Hearne ◽  
Terry C. Pellmar ◽  
...  
Keyword(s):  
Acute Radiation ◽  
Dicentric Chromosome ◽  
Mass Casualties ◽  
Acute Radiation Syndrome ◽  
Biological Dosimetry

scholarly journals Biological dosimetry by the triage dicentric chromosome assay – Further validation of international networking

2011 ◽  
Vol 46 (9) ◽  
pp. 923-928 ◽  
Author(s):  
Ruth C. Wilkins ◽  
Horst Romm ◽  
Ursula Oestreicher ◽  
Leonora Marro ◽  
Mitsuaki A. Yoshida ◽  
...  
Keyword(s):  
Dicentric Chromosome ◽  
Biological Dosimetry

Validation of the cytokinesis-block micronucleus (CBMN) assay for use as a triage biological dosimetry tool

2009 ◽  
Vol 135 (4) ◽  
pp. 232-242 ◽  
Author(s):  
J. P. McNamee ◽  
F. N. Flegal ◽  
H. B. Greene ◽  
L. Marro ◽  
R. C. Wilkins
Keyword(s):  
Biological Dosimetry ◽  
Cbmn Assay

Validation of QuickScan Dicentric Chromosome Analysis for High Throughput Radiation Biological Dosimetry

2012 ◽  
Vol 102 (2) ◽  
pp. 143-153 ◽  
Author(s):  
F. N. Flegal ◽  
Y. Devantier ◽  
L. Marro ◽  
R. C. Wilkins
Keyword(s):  
High Throughput ◽  
Chromosome Analysis ◽  
Dicentric Chromosome ◽  
Biological Dosimetry

scholarly journals Automated Cytogenetic Biodosimetry at Population-Scale

Radiation ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 79-94
Author(s):  
Peter K. Rogan ◽  
Eliseos J. Mucaki ◽  
Ben C. Shirley ◽  
Yanxin Li ◽  
Ruth C. Wilkins ◽  
...  
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Dicentric Chromosome ◽  
Automated Assignment ◽  
Simulated Population ◽  
Dose Estimation ◽  
Processing Times ◽  
Multiple Processors ◽  
Exposure Levels ◽  
Population Scale

The dicentric chromosome (DC) assay accurately quantifies exposure to radiation; however, manual and semi-automated assignment of DCs has limited its use for a potential large-scale radiation incident. The Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software automates unattended DC detection and determines radiation exposures, fulfilling IAEA criteria for triage biodosimetry. This study evaluates the throughput of high-performance ADCI (ADCI-HT) to stratify exposures of populations in 15 simulated population scale radiation exposures. ADCI-HT streamlines dose estimation using a supercomputer by optimal hierarchical scheduling of DC detection for varying numbers of samples and metaphase cell images in parallel on multiple processors. We evaluated processing times and accuracy of estimated exposures across census-defined populations. Image processing of 1744 samples on 16,384 CPUs required 1 h 11 min 23 s and radiation dose estimation based on DC frequencies required 32 sec. Processing of 40,000 samples at 10 exposures from five laboratories required 25 h and met IAEA criteria (dose estimates were within 0.5 Gy; median = 0.07). Geostatistically interpolated radiation exposure contours of simulated nuclear incidents were defined by samples exposed to clinically relevant exposure levels (1 and 2 Gy). Analysis of all exposed individuals with ADCI-HT required 0.6–7.4 days, depending on the population density of the simulation.

scholarly journals RENEB/EURADOS Field Exercise 2019: Robust Dose Estimation under outdoor Conditions based on the Dicentric Chromosome Assay

2021 ◽  
pp. 1-48
Author(s):  
David Endesfelder ◽  
Ursula Oestreicher ◽  
Ulrike Kulka ◽  
Elizabeth A. Ainsbury ◽  
Jayne Moquet ◽  
...  
Keyword(s):  
Dicentric Chromosome ◽  
Dose Estimation ◽  
Field Exercise

Detection of dicentric chromosome (9;20) in paediatric B-cell acute lymphoblastic leukaemia: prognostic significance

2014 ◽  
Vol 94 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Mathilde Letouzey ◽  
Dominique Penther ◽  
Catherine Roche-Lestienne ◽  
Brigitte Nelken ◽  
Catherine Devoldère ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukaemia ◽  
B Cell ◽  
Lymphoblastic Leukaemia ◽  
Prognostic Significance ◽  
Dicentric Chromosome
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