Validation of QuickScan Dicentric Chromosome Analysis for High Throughput Radiation Biological Dosimetry

2012 ◽  
Vol 102 (2) ◽  
pp. 143-153 ◽  
Author(s):  
F. N. Flegal ◽  
Y. Devantier ◽  
L. Marro ◽  
R. C. Wilkins
Keyword(s):  
High Throughput ◽  
Chromosome Analysis ◽  
Dicentric Chromosome ◽  
Biological Dosimetry

Biological Dosimetry by the Triage Dicentric Chromosome Assay: Potential Implications for Treatment of Acute Radiation Syndrome in Radiological Mass Casualties

2011 ◽  
Vol 175 (3) ◽  
pp. 397-404 ◽  
Author(s):  
Horst Romm ◽  
Ruth C. Wilkins ◽  
C. Norman Coleman ◽  
Patricia K. Lillis-Hearne ◽  
Terry C. Pellmar ◽  
...  
Keyword(s):  
Acute Radiation ◽  
Dicentric Chromosome ◽  
Mass Casualties ◽  
Acute Radiation Syndrome ◽  
Biological Dosimetry

QUICKSCAN DICENTRIC CHROMOSOME ANALYSIS FOR RADIATION BIODOSIMETRY

2010 ◽  
Vol 98 (2) ◽  
pp. 276-281 ◽  
Author(s):  
F N. Flegal ◽  
Y Devantier ◽  
J P. McNamee ◽  
R C. Wilkins
Keyword(s):  
Chromosome Analysis ◽  
Dicentric Chromosome ◽  
Radiation Biodosimetry

scholarly journals Optimization and validation of automated dicentric chromosome analysis for radiological/nuclear triage applications

2019 ◽  
Vol 847 ◽  
pp. 503087 ◽  
Author(s):  
Terri L. Ryan ◽  
Maria B. Escalona ◽  
Tammy L. Smith ◽  
Joseph Albanese ◽  
Carol J. Iddins ◽  
...  
Keyword(s):  
Chromosome Analysis ◽  
Dicentric Chromosome

scholarly journals Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis

2013 ◽  
Vol 36 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Yuchen Qiu ◽  
Xiaodong Chen ◽  
Yuhua Li ◽  
Wei R. Chen ◽  
Bin Zheng ◽  
...  
Keyword(s):  
Bone Marrow ◽  
High Throughput ◽  
Metaphase Chromosome ◽  
Imaging Modality ◽  
Digital Pathology ◽  
Chromosome Analysis ◽  
Blood Specimens ◽  
Time Accuracy ◽  
High Throughput Imaging ◽  
Auto Focusing

Background: Auto-focusing is an important operation in high throughput imaging scanning. Although many auto-focusing methods have been developed and tested for a variety of imaging modalities, few investigations have been performed on the selection of an optimal auto-focusing method that is suitable for the pathological metaphase chromosome analysis under a high resolution scanning microscopic system.Objective: The purpose of this study is to investigate and identify an optimal auto-focusing method for the pathological metaphase chromosome analysis.Methods: In this study, five auto-focusing methods were applied and tested using metaphase chromosome images acquired from bone marrow and blood specimens. These methods were assessed by measuring a number of indices including execution time, accuracy, number of false maxima, and full width at half maximum (FWHM).Results: For the specific condition investigated in this study, the results showed that the Brenner gradient and threshold pixel counting methods were the optimal methods for acquiring high quality metaphase chromosome images from the bone marrow and blood specimens, respectively.Conclusions: Selecting an optimal auto-focusing method depends on the specific clinical tasks. This study also provides useful information for the design and implementation of the high throughput microscopic image scanning systems in the future digital pathology.

scholarly journals Biological dosimetry by the triage dicentric chromosome assay – Further validation of international networking

2011 ◽  
Vol 46 (9) ◽  
pp. 923-928 ◽  
Author(s):  
Ruth C. Wilkins ◽  
Horst Romm ◽  
Ursula Oestreicher ◽  
Leonora Marro ◽  
Mitsuaki A. Yoshida ◽  
...  
Keyword(s):  
Dicentric Chromosome ◽  
Biological Dosimetry

Laboratory Intercomparison of the Dicentric Chromosome Analysis Assay

2013 ◽  
Vol 180 (2) ◽  
pp. 129-137 ◽  
Author(s):  
C. Beinke ◽  
S. Barnard ◽  
H. Boulay-Greene ◽  
A. De Amicis ◽  
S. De Sanctis ◽  
...  
Keyword(s):  
Chromosome Analysis ◽  
Dicentric Chromosome ◽  
Laboratory Intercomparison

scholarly journals Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool

2015 ◽  
Vol 184 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Stanley W. Lue ◽  
Mikhail Repin ◽  
Ryan Mahnke ◽  
David J. Brenner
Keyword(s):  
High Throughput ◽  
Micronucleus Assay ◽  
Biological Dosimetry

scholarly journals Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms

2020 ◽  
Vol 10 (9) ◽  
pp. 3299-3307 ◽  
Author(s):  
R Rebecca Love ◽  
Marco Pombi ◽  
Moussa W Guelbeogo ◽  
Nathan R Campbell ◽  
Melissa T Stephens ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
High Throughput ◽  
Natural Populations ◽  
Chromosome Analysis ◽  
Special Importance ◽  
Nucleotide Polymorphisms ◽  
Molecular Genotyping ◽  
Gambiae Complex ◽  
Tag Snps ◽  
Anopheles Gambiae Complex

Abstract Chromosomal inversion polymorphisms have special importance in the Anopheles gambiae complex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. The study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. To overcome this barrier, we ascertained tag single nucleotide polymorphisms (SNPs) that are highly correlated with inversion status (inverted or standard orientation). We compared the performance of the tag SNPs using two alternative high throughput molecular genotyping approaches vs. traditional cytogenetic karyotyping of the same 960 individual An. gambiae and An. coluzzii mosquitoes sampled from Burkina Faso, West Africa. We show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. Given the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag SNPs has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation of An. gambiae and An. coluzzii to environmental heterogeneities.

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