Development of Autonomic Innervation in Mammalian Myocardium

Developmental Neurobiology of the Autonomic Nervous System ◽

10.1385/0-89603-080-6:159 ◽

2003 ◽

pp. 159-192 ◽

Cited By ~ 2

Author(s):

Howard L. Cohen

Keyword(s):

Autonomic Innervation ◽

Mammalian Myocardium

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Autonomic innervation during development of the rat submandibular gland.

Japanese Journal of Oral Biology ◽

10.2330/joralbiosci1965.26.1023 ◽

1984 ◽

Vol 26 (4) ◽

pp. 1023-1039

Author(s):

Teruyoshi Kondo

Keyword(s):

Submandibular Gland ◽

Autonomic Innervation ◽

Rat Submandibular Gland

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Faculty Opinions recommendation of Noninvasive in vivo model demonstrating the effects of autonomic innervation on pancreatic islet function.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717975914.793470333 ◽

2013 ◽

Author(s):

Andries Kalsbeek

Keyword(s):

Pancreatic Islet ◽

Autonomic Innervation ◽

In Vivo Model ◽

Islet Function

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AUTONOMIC INNERVATION OF FELINE LARYNGEAL GLANDS

Nippon Jibiinkoka Gakkai Kaiho ◽

10.3950/jibiinkoka.96.1311 ◽

1993 ◽

Vol 96 (8) ◽

pp. 1311-1319,1391 ◽

Cited By ~ 2

Author(s):

YASUMASA TANAKA ◽

YOSHIKAZU YOSHIDA ◽

MINORU HIRANO ◽

SETSUJI HISANO ◽

SHIGEO DAIKOKU

Keyword(s):

Autonomic Innervation

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Effects of acidosis on resting cytosolic and mitochondrial Ca2+ in mammalian myocardium.

The Journal of General Physiology ◽

10.1085/jgp.102.3.575 ◽

1993 ◽

Vol 102 (3) ◽

pp. 575-597 ◽

Cited By ~ 26

Author(s):

G Gambassi ◽

R G Hansford ◽

S J Sollott ◽

B A Hogue ◽

E G Lakatta ◽

...

Keyword(s):

Lactic Acid ◽

Ventricular Myocytes ◽

Free Acid ◽

Left Ventricular ◽

Ruthenium Red ◽

Acetoxymethyl Ester ◽

Cytosolic Ph ◽

Bicarbonate Buffer ◽

Adult Rat ◽

Mammalian Myocardium

Acidosis increases resting cytosolic [Ca2+], (Cai) of myocardial preparations; however, neither the Ca2+ sources for the increase in Cai nor the effect of acidosis on mitochondrial free [Ca2+], (Cam) have been characterized. In this study cytosolic pH (pHi) was monitored in adult rat left ventricular myocytes loaded with the acetoxymethyl ester (AM form) of SNARF-1. A stable decrease in the pHi of 0.52 +/- 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. Electrical stimulation at either 0.5 or 1.5 Hz had no effect on pHi in 5% CO2, nor did it affect the magnitude of pHi decrease in response to hypercarbic acidosis. Cai was measured in myocytes loaded with indo-1/free acid and Cam was monitored in cells loaded with indo-1/AM after quenching cytosolic indo-1 fluorescence with MnCl2. In quiescent intact myocytes bathed in 1.5 mM [Ca2+], hypercarbia increased Cai from 130 +/- 5 to 221 +/- 13 nM. However, when acidosis was effected in electrically stimulated myocytes, diastolic Cai increased more than resting Cai in quiescent myocytes, and during pacing at 1.5 Hz diastolic Cai was higher (285 +/- 17 nM) than at 0.5 Hz (245 +/- 18 nM; P < 0.05). The magnitude of Cai increase in quiescent myocytes was not affected either by sarcoplasmic reticulum (SR) Ca2+ depletion with ryanodine or by SR Ca2+ depletion and concomitant superfusion with a Ca(2+)-free buffer. In unstimulated intact myocytes hypercarbia increased Cam from 95 +/- 12 to 147 +/- 19 nM and this response was not modified either by ryanodine and a Ca(2+)-free buffer or by 50 microM ruthenium red in order to block the mitochondrial uniporter. In mitochondrial suspensions loaded either with BCECF/AM or indo-1/AM, acidosis produced by lactic acid addition decreased both intra- and extramitochondrial pH and increased Cam. Studies of mitochondrial suspensions bathed in indo-1/free acid-containing solution showed an increase in extramitochondrial Ca2+ after the addition of lactic acid. Thus, in quiescent myocytes, cytoplasmic and intramitochondrial buffers, rather than transsarcolemmal Ca2+ influx or SR Ca2+ release, are the likely Ca2+ sources for the increase in Cai and Cam, respectively; additionally, Ca2+ efflux from the mitochondria may contribute to the raise in Cai. In contrast, in response to acidosis, diastolic Cai in electrically stimulated myocytes increases more than resting Cai in quiescent cells; this suggests that during pacing, net cell Ca2+ gain contributes to enhance diastolic Cai.

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