CelluSpots™: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity

Author(s):  
Chenggang Wu ◽  
Shawn S.-C. Li
1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S104-S121 ◽  
Author(s):  
E. E. Baulieu ◽  
J. P. Raynaud ◽  
E. Milgrom

ABSTRACT A brief review of the characteristics of steroid binding proteins found in the plasma and in some target organs is presented, followed by some general remarks on binding »specificity« and binding parameters. Useful techniques for measuring binding parameters at equilibrium are reported, both those which keep the equilibrium intact and those which implicate its disruption. A concept is developed according to which the determination of a specific steroid binding protein is based on the »differential dissociation« of the several steroid binding complexes present in most biological mixtures. Methods which allow determination of the kinetic parameters of the binding systems are also presented. Various representations of the binding and therefore different modes of graphic representation and calculation are discussed, including the recent »proportion graph« method.


1992 ◽  
Vol 22 (6) ◽  
pp. 1405-1412 ◽  
Author(s):  
Ton N. M. Schumacher ◽  
Grada M. Van Bleek ◽  
Marie-ThéRèSe Heemels ◽  
Karl Deres ◽  
Ka Wan Li ◽  
...  

2011 ◽  
Vol 12 (1) ◽  
pp. 48 ◽  
Author(s):  
Ina Bock ◽  
Srikanth Kudithipudi ◽  
Raluca Tamas ◽  
Goran Kungulovski ◽  
Arunkumar Dhayalan ◽  
...  

1999 ◽  
Vol 274 (3) ◽  
pp. 1342-1348 ◽  
Author(s):  
Serge Haan ◽  
Ulrike Hemmann ◽  
Ulrich Hassiepen ◽  
Fred Schaper ◽  
Jens Schneider-Mergener ◽  
...  

2008 ◽  
Vol 2008 (4) ◽  
pp. pdb.prot4972-pdb.prot4972 ◽  
Author(s):  
A. J. Gossett ◽  
J. D. Lieb

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