scholarly journals Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells In Situ

2012 ◽  
pp. 245-254 ◽  
Author(s):  
Saumil Sethna ◽  
Silvia C. Finnemann
Keyword(s):  
Outer Segment ◽  
Rpe Cells ◽  
Rod Outer Segment

Kinetic studies of rod outer segment binding and ingestion by cultured rat RPE cells

1987 ◽  
Vol 45 (6) ◽  
pp. 907-922 ◽  
Author(s):  
Michael O. Hall ◽  
Toshka Abrams
Keyword(s):  
Outer Segment ◽  
Kinetic Studies ◽  
Rpe Cells ◽  
Rod Outer Segment

scholarly journals Localization of rhodopsin in isolated, osmotically intact rod outer segment discs.

1979 ◽  
Vol 27 (3) ◽  
pp. 788-793 ◽  
Author(s):  
D I Blaustein ◽  
M M Dewey
Keyword(s):  
Horseradish Peroxidase ◽  
Outer Segment ◽  
Rod Outer Segments ◽  
Specific Antibodies ◽  
Rod Outer Segment ◽  
Mechanical Interference ◽  
Immunocytochemical Techniques ◽  
The Subject ◽  
Peroxidase Conjugate

Localization of rhodopsin and its position in the membrane has been the subject of numerous studies. Most recently, immunocytochemical techniques have been employed to localize the opsin component of the molecule in in situ rod outer segments. Due to the problems inherent in localization procedures (penetration and mechanical interference) we have utilized isolated, osmotically intact rod outer segment discs in this study. Specific antibodies to chromatographically pure rhodopsin were prepared and enzymatically digested to their Fab components. The univalent Fab antibodies were conjugated to horseradish peroxidase and used to label the isolated rod outer segment discs. Discs treated with anti-opsin conjugate stained uniformly and heavily on their interdisc surfaces. Reaction product was also present on the intradisc surface in a thinner but still uniformly distributed layer. Controls treated with preimmune Fab - horseradish peroxidase conjugate showed no deposition of reaction product.

High time resolution enzyme cytochemistry of rod outer segment

1990 ◽  
Vol 48 (3) ◽  
pp. 878-879
Author(s):  
Takuma Saito ◽  
Toshihiro Takizawa
Keyword(s):  
Enzyme Activities ◽  
Outer Segment ◽  
Rapid Change ◽  
High Time Resolution ◽  
Visual Cell ◽  
Enzyme Cytochemistry ◽  
Activation Effect ◽  
Rod Outer Segment ◽  
Enzymatic Cascades ◽  
Rapid Drop

Cells and tissues live on a number of dynamic metabolic pathways, which are made up of sequential enzymatic cascades.Recent biochemical and physiological studies of vision research showed the importance of cGMP metabolism in the rod outer segment of visual cell, indicat ing that the photon activated rhodopsin exerts activation effect on the GTP binding protein, transducin, and this act ivated transducin further activates phosphodiesterase (PDEase) to result in a rapid drop in cGMP concentration in the cytoplasm of rod outer segment. This rapid drop of cGMP concentration exerts to close the ion channel on the plasma membrane and to stop of inward current brings hyperpolarization and evokes an action potential.These sequential change of enzyme activities, known as cGMP cascade, proceeds quite rapidly within msec order. Such a rapid change of enzyme activities, such as PDEase in rod outer segment, was not a matter of conventional histochemical invest igations.

Sorbitol, myo-inositol, and rod outer segment phagocytosis in cultured hRPE cells exposed to glucose. In vitro model of myo-inositol depletion hypothesis of diabetic complications

Diabetes ◽  
1991 ◽  
Vol 40 (10) ◽  
pp. 1335-1345 ◽  
Author(s):  
M. A. Del Monte ◽  
R. Rabbani ◽  
T. C. Diaz ◽  
S. A. Lattimer ◽  
J. Nakamura ◽  
...  
Keyword(s):  
Diabetic Complications ◽  
Outer Segment ◽  
In Vitro Model ◽  
Rod Outer Segment ◽  
Vitro Model
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