Fusion Complementation Tests on a Group of Independently-Isolated Auxotrophic and Temperature-Sensitive Clones of Hyoscyamus Muticus and Nicotiana Tabacum

1983 ◽  
pp. 112-113 ◽  
Author(s):  
H. Fankhauser ◽  
C. Gebhardt ◽  
Jia Jing-Fen ◽  
P. J. King ◽  
M. Laser ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Temperature Sensitive ◽  
Hyoscyamus Muticus

scholarly journals TEMPERATURE-SENSITIVE VARIANTS OF NICOTIANA TABACUM ISOLATED FROM SOMATIC CELL CULTURE

Genetics ◽  
1979 ◽  
Vol 92 (1) ◽  
pp. 215-221
Author(s):  
Russell L Malmberg
Keyword(s):  
Nicotiana Tabacum ◽  
Suspension Culture ◽  
Selection Procedure ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Permissive Temperature ◽  
Temperature Growth ◽  
Liquid Suspension ◽  
Slow Growing ◽  
Liquid Suspension Culture

ABSTRACT Temperature-sensitive variants of Nicotiana tabacum were isolated from a liquid suspension culture of somatic cells by a negative selection procedure, using bromodeoxyuridine and light. A total of nine such variants have been recovered, with an estimated rate of 2 × 10-7 per cell division. The appearance of the variants at the permissive temperature varied from nearly wild type, white and friable, to brown, compact and slow growing. Two of the variants adapted from growth on solid medium to growth in a liquid suspension culture; these were further characterized for chromosome number, growth rate, cell death rate at the restrictive temperature, growth on nutritionally modified media, and RNA and protein synthesis. The variants have been placed on regeneration media, and one of them has produced plantlets. Leaves from a plantlet have been placed on callus-inducing media, and the resulting callus displayed the temperature-sensitive phenotype.

Hyoscyamus muticus + Nicotiana tabacum fusion hybrids selected via auxotroph complementation

1984 ◽  
Vol 3 (2) ◽  
pp. 68-71 ◽  
Author(s):  
I. Potrykus ◽  
J. Jia ◽  
G. B. Lazar ◽  
M. Saul
Keyword(s):  
Nicotiana Tabacum ◽  
Hyoscyamus Muticus

Oncogenesis and the Lucké Virus

1968 ◽  
Vol 26 ◽  
pp. 200-201
Author(s):  
A. E. Vatter ◽  
J. Zambernard
Keyword(s):  
Rna Viruses ◽  
Vegetative State ◽  
Temperature Sensitive ◽  
Structural Level ◽  
Oncogenic Viruses ◽  
Dna Viruses ◽  
Leopard Frogs ◽  
Renal Adenocarcinoma ◽  
Induced Tumors ◽  
Northern Leopard Frogs

Oncogenic viruses, like viruses in general, can be divided into two classes, those that contain deoxyribonucleic acid (DNA) and those that contain ribonucleic acid (RNA). The RNA viruses have been recovered readily from the tumors which they cause whereas, the DNA-virus induced tumors have not yielded the virus. Since DNA viruses cannot be recovered, the bulk of present day investigations have been concerned with RNA viruses.The Lucké renal adenocarcinoma is a spontaneous tumor which occurs in northern leopard frogs (Rana pipiens) and has received increased attention in recent years because of its probable viral etiology. This hypothesis was first advanced by Lucké after he observed intranuclear inclusions in some of the tumor cells. Tumors with inclusions were examined at the fine structural level by Fawcett who showed that they contained immature and mature virus˗like particles.The use of this system in the study of oncogenic tumors offers several unique features, the virus has been shown to contain DNA and it can be recovered from the tumor, also, it is temperature sensitive. This latter feature is of importance because the virus can be transformed from a latent to a vegetative state by lowering or elevating the environmental temperature.

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