A Bioprinted Heart-on-a-Chip with Human Pluripotent Stem Cell-Derived Cardiomyocytes for Drug Evaluation

In this work, we show that valve-based bioprinting induces no measurable detrimental effects on human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The aim of the current study was three-fold: first, to assess the response of hiPSC-CMs to several hydrogel formulations by measuring electrophysiological function; second, to customise a new microvalve-based cell printing mechanism in order to deliver hiPSC-CMs suspensions, and third, to compare the traditional manual pipetting cell-culture method and cardiomyocytes dispensed with the bioprinter. To achieve the first and third objectives, iCell2 (Cellular Dynamics International) hiPSC-CMs were used. The effects of well-known drugs were tested on iCell2 cultured by manual pipetting and bioprinting. Despite the results showing that hydrogels and their cross-linkers significantly reduced the electrophysiological performance of the cells compared with those cultured on fibronectin, the bio-ink droplets containing a liquid suspension of live cardiomyocytes proved to be an alternative to standard manual handling and could reduce the number of cells required for drug testing, with no significant differences in drug-sensitivity between both approaches. These results provide a basis for the development of a novel bioprinter with nanolitre resolution to decrease the required number of cells and to automate the cell plating process.

Numerical analysis and optimization of key parts in the stirred tank based on solid-liquid flow field

In order to further improve the mixing performance of the mixing device, the structure of the agitator was optimized, and the effects of the diameter and pitch of the agitator on the solid-liquid suspension characteristics were analyzed by single factor method. Multiple reference frame (MRF), computational fluid dynamics, Euler multiphase flow model and standard K- ε turbulence model were used to investigate the effect of the height from the bottom of the agitator on the suspension characteristics of particles in the agitator was studied. The results show that reducing the height from the bottom of the agitator can promote the suspension of particles at the bottom of the tank, but too low height from the bottom will easily produce mixing dead zone at the bottom of the tank, and cause the accumulation of particles. Reducing the height of the agitator from the bottom will enlarge the clear liquid area of the flow field, cause uneven particle distribution and increase the stirring torque. With the increase of agitator diameter, the critical suspension speed of the flow field decrease, but the stirring power required by the flow field increase. Increasing the blade spacing in a certain range can promote the suspension of particles and make the distribution of particles in the flow field more uniform. Therefore, the mixing power and the uniformity of particle concentration distribution need to be considered together in order to make the mixing device more efficient and energy-saving.

Electro-osmosis modulated biologically inspired flow of solid liquid suspension in a channel with complex progressive wave: An application of targeted drugging

Electrokinetic microperistaltic pumps are important biomechanical devices, helps in targeted drugging of sick body parts. The current article is written to focus on mathematical modelling and analysis of some important aspect of such flows in a channel with complex wave. It is considered that solid partilcle are uniformly distributed in the flow and these particle are non-conducting. Parameters such as Particle volume fraction coefficient, Electro-osmotic parameter and Helmholtz-Smoluchowski parameter are specially been focused in this study. Spherical shaped equally sized are uniformly floated in a non-Newtonian Powell-Eyring base fluid. The defined flow problem is modelled and analyzed analytically for the transport of solid liquid suspension. It is accepted that the flow is steady, nonturbulent and propagating waves do have a considerably longer wave-length when compared to amplitude. The conditions and assumptions lead to a model of coupled partial differential equations of order two. The exact results by HPM expansion method are procured and shown accordingly. The predictions about the behaviour of important appearing parameters are displayed using figures. The impact of sundry parameters are analyzed. The application of current study involved transporting/ targeted drug delivery system using Peristaltic micropumps and magnetic field in pharmacological engineering of biofluids like blood.

Correlative Analysis of the Dimensional Properties of Bipyramidal Titania Nanoparticles by Complementing Electron Microscopy with Other Methods

In this paper, the accurate determination of the size and size distribution of bipyramidal anatase nanoparticles (NPs) after deposition as single particles on a silicon substrate by correlative Scanning Electron Microscopy (SEM) with Atomic Force Microscopy (AFM) analysis is described as a new measurement procedure for metrological purposes. The knowledge of the exact orientation of the NPs is a crucial step in extracting the real 3D dimensions of the particles. Two approaches are proposed to determine the geometrical orientation of individual nano-bipyramides: (i) AFM profiling along the long bipyramid axis and (ii) stage tilting followed by SEM imaging. Furthermore, a recently developed method, Transmission Kikuchi Diffraction (TKD), which needs preparation of the crystalline NPs on electron-transparent substrates such as TEM grids, has been tested with respect to its capability of identifying the geometrical orientation of the individual NPs. With the NPs prepared homogeneously on a TEM grid, the transmission mode in a SEM, i.e., STEM-in-SEM (or T-SEM), can be also applied to extract accurate projection dimensions of the nanoparticles from the same sample area as that analysed by SEM, TKD and possibly AFM. Finally, Small Angle X-ray Scattering (SAXS) can be used as an ensemble technique able to measure the NPs in liquid suspension and, with ab-initio knowledge of the NP shape from the descriptive imaging techniques, to provide traceable NP size distribution and particle concentration.

Study of Novel Punched-Bionic Impellers for High Efficiency and Homogeneity in PCM Mixing and Other Solid-Liquid Stirs

Improvement of stirring performance is one of the primary objectives in solid–liquid mixing processes, such as the preparation of phase change materials (PCMs) for energy saving in refrigeration and heat pump systems. In this paper, three novel impellers are proposed: pitched-blade punched turbine (PBPT), bionic cut blade turbine (BCBT) and bionic cut punched blade turbine (BCPBT). An experimental test was conducted to validate the stirring system model based on the Eulerian–Eulerian method with the kinetic theory of granular flow. Then the performance of the novel impellers was predicted, studied, and compared. The outcomes indicate that a novel impeller, specifically BCPBT, can effectively suspend particles and dramatically reduce power consumption. A better solid–liquid suspension quality was obtained with an aperture diameter of 8 mm and aperture ratio of 13%. Within the range of impeller speeds and liquid viscosity studied in this this paper, higher impeller speeds and more viscous liquids are more conducive to particle dispersion. One of the most important contributions of this work lies in the design of novel impellers, an extent of energy conservation to 17% and efficient mixing was achieved. These results have reference significance for improving the energy efficiency of temperature regulation systems.

Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Background Liquid suspension culture efficiently proliferates plant cells and can be applied to ferns because it rapidly increases the fresh weight of gametophytes. This study assessed gametophyte proliferation and sporophyte production of Pteridium aquilinum var. latiusculum using a suspension culture method. Results The growth curve linear phase of gametophyte cells was confirmed between 9 and 18 days of culture, and the subculture cycle was determined to be 2 weeks. A double-strength MS medium (fresh weight, 18.0 g) containing 2% sucrose and NH4+:NO3− (120 mM, 40:80) was found to be the optimal liquid medium. Gametophytes obtained after suspension culture for 18 days did not normally form sporophytes in an ex vitro soil environment. However, this issue was resolved after changing the culture type or extending the culture period to 6 weeks. A short suspension culture period increased the fresh weight of fragmented and homogenized gametophytes but yielded numerous relatively immature gametophytes (globular forms of branching gametophytes, BG). Furthermore, differences in gametophyte morphogenesis and development were indicated by changes in endogenous phytohormone content. BG with immature development exhibited high accumulation of zeatin, jasmonic acid, and salicylic acid, and relatively low levels of abscisic acid and indole-3-acetic acid. The immature development of gametophytes directly affected sporophyte formation. Conclusions This study maximized the advantages of liquid suspension culture using eastern bracken gametophytes and provides data to resolve any associated issues, thus facilitating efficient bracken production.