Image Analysis of Gene Locus Positions Within Chromosome Territories in Human Lymphocytes

2014 ◽  
pp. 125-134
Author(s):  
Karel Štěpka ◽  
Martin Falk
Keyword(s):  
Image Analysis ◽  
Gene Locus ◽  
Human Lymphocytes ◽  
Chromosome Territories

scholarly journals Scoring of radiation-induced microuclei in cytokinesis-blocked human lymphocytes by automated image analysis

Cytometry ◽  
1994 ◽  
Vol 17 (2) ◽  
pp. 119-127 ◽  
Author(s):  
F. Verhaegen ◽  
A. Vral ◽  
J. Seuntjens ◽  
N. W. Schipper ◽  
L. de Ridder ◽  
...  
Keyword(s):  
Image Analysis ◽  
Human Lymphocytes ◽  
Automated Image Analysis ◽  
Radiation Induced

Quantitative assessment of bleomycin-induced poly(ADP-ribosyl)ation in human lymphocytes by immunofluorescence and image analysis

2000 ◽  
Vol 244 (1-2) ◽  
pp. 145-151 ◽  
Author(s):  
Nahid Rajaee-Behbahani ◽  
Peter Schmezer ◽  
Alexander Bürkle ◽  
Helmut Bartsch
Keyword(s):  
Image Analysis ◽  
Quantitative Assessment ◽  
Human Lymphocytes

The use of an image analysis system for the automated screening of in vitro induced micronuclei on human lymphocytes

1991 ◽  
Vol 73 (2-3) ◽  
pp. 30a-30a
Author(s):  
Philippe Castelain ◽  
Alain Deleener ◽  
Micheline Kirsch-Volders
Keyword(s):  
Image Analysis ◽  
Human Lymphocytes ◽  
Image Analysis System ◽  
Automated Screening ◽  
Analysis System

Automatic analysis of the micronucleus test in primary human lymphocytes using image analysis

Mutagenesis ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 15-23 ◽  
Author(s):  
W. Frieauff ◽  
H. J. Martus ◽  
W. Suter ◽  
A. Elhajouji
Keyword(s):  
Image Analysis ◽  
Micronucleus Test ◽  
Human Lymphocytes ◽  
Automatic Analysis

scholarly journals The G0 ⇆ G1 transitions of human lymphocytes as monitored by quantitative14C-uridine autoradiography and high-resolution image analysis

Cytometry ◽  
1985 ◽  
Vol 6 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Walter Giaretti ◽  
Wolfgang Abmayr ◽  
Peter Dörmer ◽  
Leonardo Santi
Keyword(s):  
Image Analysis ◽  
High Resolution ◽  
Human Lymphocytes ◽  
Resolution Image ◽  
High Resolution Image

scholarly journals Distinct patterns of histone methylation and acetylation in human interphase nuclei

2007 ◽  
pp. 797-806
Author(s):  
M Skalníková ◽  
E Bártová ◽  
V Ulman ◽  
P Matula ◽  
D Svoboda ◽  
...  
Keyword(s):  
Image Analysis ◽  
Histone Modifications ◽  
Histone Methylation ◽  
Nuclear Periphery ◽  
Chromosome Territories ◽  
Interphase Nuclei ◽  
Peripheral Part ◽  
Total Fluorescence ◽  
Acrocentric Chromosomes ◽  
Histone Methylation And Acetylation

To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyltransferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analyzed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (di-me) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1. The histone modifications studied were absent in the nucleolar compartment with the exception of H3(K9) dimethylation that was closely associated with perinucleolar regions which are formed by centromeres of acrocentric chromosomes. Using immunocytochemistry, no di-meH3(K4) but only dense di-meH3(K9) was found for the human acrocentric chromosomes 14 and 22. The active X chromosome was observed to be partially acetylated, while the inactive X was more condensed, located in a very peripheral part of the interphase nuclei, and lacked H3(K9) acetylation. Our results confirmed specific interphase patterns of histone modifications within the interphase nuclei as well as within their chromosome territories.

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