Asymmetric Somatic Hybrids

1994 ◽  
pp. 57-71 ◽  
Author(s):  
S. Hinnisdaels ◽  
M. Jacobs ◽  
I. Negrutiu
Keyword(s):  
Somatic Hybrids ◽  
Asymmetric Somatic Hybrids

UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana

Plant Science ◽  
1998 ◽  
Vol 131 (1) ◽  
pp. 65-76 ◽  
Author(s):  
Johanna Forsberg ◽  
Christina Dixelius ◽  
Ulf Lagercrantz ◽  
Kristina Glimelius
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Somatic Hybrids ◽  
Asymmetric Somatic Hybrids ◽  
Dna Elimination ◽  
Uv Dose ◽  
Dose Dependent

Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization

1992 ◽  
Vol 2 (6) ◽  
pp. 863-874 ◽  
Author(s):  
A. S. Parokonny ◽  
A. Y. Kenton ◽  
Y. Y. Gleba ◽  
M. D. Bennett
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Asymmetric Somatic Hybrids ◽  
Genome Reorganization

The fate of recombinant chromosomes and genome interaction in Nicotiana asymmetric somatic hybrids and their sexual progeny

1994 ◽  
Vol 89 (4) ◽  
pp. 488-497 ◽  
Author(s):  
A. S. Parokonny ◽  
A. Kenton ◽  
Y. Y. Gleba ◽  
M. D. Bennett
Keyword(s):  
Somatic Hybrids ◽  
Asymmetric Somatic Hybrids ◽  
Sexual Progeny

Cytogenetic characterization and fae1 gene variation in progenies from asymmetric somatic hybrids between Brassica napus and Crambe abyssinica

Genome ◽  
2004 ◽  
Vol 47 (4) ◽  
pp. 724-731 ◽  
Author(s):  
Y P Wang ◽  
R J Snowdon ◽  
E Rudloff ◽  
P Wehling ◽  
W Friedt ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Genetic Material ◽  
Asymmetric Somatic Hybrids ◽  
Crambe Abyssinica ◽  
Gene Variation ◽  
Cytogenetic Characterization ◽  
Sexual Progeny ◽  
Chromatin Bridges

Sexual progenies of asymmetric somatic hybrids between Brassica napus and Crambe abyssinica were analyzed with respect to chromosomal behavior, fae1 gene introgression, fertility, and fatty-acid composition of the seed. Among 24 progeny plants investigated, 11 plants had 38 chromosomes and were characterized by the occurrence of normal meiosis with 19 bivalents. The other 13 plants had more than 38 chromosomes, constituting a complete chromosomal set from B. napus plus different numbers of additional chromosomes from C. abyssinica. The chromosomes of B. napus and C. abyssinica origin could be clearly discriminated by genomic in situ hybridization (GISH) in mitotic and meiotic cells. Furthermore, meiotic GISH enabled identification of intergenomic chromatin bridges and of asynchrony between the B. napus and C. abyssinca meiotic cycles. Lagging, bridging and late disjunction of univalents derived from C. abyssinica were observed. Analysis of cleaved amplified polymorphic sequence (CAPS) markers derived from the fae1 gene showed novel patterns different from the B. napus recipient in some hybrid offspring. Most of the progeny plants had a high pollen fertility and seed set, and some contained significantly greater amounts of seed erucic acid than the B. napus parent. This study demonstrates that a part of the C. abyssinica genome can be transferred into B. napus via asymmetric hybridization and maintained in sexual progenies of the hybrids. Furthermore, it confirms that UV irradiation improves the fertility of the hybrid and of its sexual progeny via chromosomal elimination and facilitates the introgression of exotic genetic material into crop species.Key words: asymmetric somatic hybrid, Brassica napus, Crambe abyssinica, cytogenetics, fae1 gene.

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