Limitations of Melting Curve Analysis Using SYBR Green I — Fragment Differentiation and Mutation Detection in the CFTR-Gene

2002 ◽  
pp. 47-56 ◽  
Author(s):  
S. Kleinle ◽  
K. Tabiti ◽  
S. Gallati
Keyword(s):  
Mutation Detection ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Cftr Gene ◽  
Melting Curve Analysis

Molecular detection of Mycoplasma haemomuris subspecies using dnaK-targeted real-time PCR with SYBR Green I and melting curve analysis

2016 ◽  
Vol 30 (5) ◽  
pp. 291-293
Author(s):  
Hiroshi Satoh ◽  
Daiki Imai ◽  
Shigeru Sato ◽  
Kazuhisa Furuhama ◽  
Ryô Harasawa
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Molecular Detection ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis

scholarly journals Simultaneous Genotyping of GSTT1 and GSTM1 Null Polymorphisms by Melting Curve Analysis in Presence of SYBR Green I

2010 ◽  
Vol 12 (3) ◽  
pp. 300-304 ◽  
Author(s):  
Fátima Marín ◽  
Nadia García ◽  
Xavier Muñoz ◽  
Gabriel Capellà ◽  
Carlos A. González ◽  
...  
Keyword(s):  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis

Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66

2015 ◽  
Vol 29 (1) ◽  
pp. 13-18 ◽  
Author(s):  
D. Tsakogiannis ◽  
M. Papacharalampous ◽  
E. Toska ◽  
Z. Kyriakopoulou ◽  
T.G. Dimitriou ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Identification ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis ◽  
Pcr Assay ◽  
Hpv Genotypes

scholarly journals Genotyping of Entamoeba histolytica by Real-Time Polymerase Chain Reaction with Sybr Green I and Melting Curve Analysis

1970 ◽  
Vol 4 (1) ◽  
pp. 53-60
Author(s):  
SMM Rahman ◽  
R Haque ◽  
S Roy ◽  
MMH Mondal
Keyword(s):  
Real Time ◽  
Melting Temperature ◽  
Entamoeba Histolytica ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis ◽  
Intestinal Amoebiasis

In this study, for the detection of distinct genotype of E. histolytica of human, a nested Real-Time PCR amplification of SREHP gene using SYBR Green I and melting curve analysis was done. A total of 60 specimens (stool and liver aspirate specimens), which were found Entamoeba histolytica positive by E. histolytica specific ELISA and ssrRNA gene PCR, were selected and the experiment was conducted during the period of July 2003 to June 2004. After melting curve analysis of amplified PCR products from these isolates of stool and liver aspirate specimens, 5 genotypes were found belonging to the melting temperatures 84°C, 83°C, 82°C, 81°C and 79°C. All these 5 genotypes were present in intestinal amoebiasis patients and when the genotypes from intestinal amoebiasis patients were compared with the genotypes of amoebic liver amoebiasis patients, the genotype 84°C melting temperature was found to be absent in amoebic liver amoebiasis patients. For both the cases of intestinal and amoebic liver amoebiasis patients the genotype belonging to 83°C melting temperature was more prevalent than the other genotypes which suggest that this genotype is more responsible for the development of amoebiasis. In comparison to conventional PCR method where we found 23 different banding patterns, the Real-Time PCR and melting curve analysis method was found to be more reliable for the detection of distinct genotypes of E. histolytica because with this method we found only 5 genotypes. In conclusion, this Real-Time PCR using SYBR Green I and melting curve analysis for the genotyping of E. histolytica, excludes the need of post PCR manipulations and would be helpful for the rapid detection and screening of E. histolytica genotypes among endemic population and also for the epidemiological study. Key words: Entamoeba histolytica, genotype, diarrhoea, Real-Time PCR, melting curve analysis doi:10.3329/bjvm.v4i1.1526 Bangl. J. Vet. Med. (2006). 4 (1): 53-60

scholarly journals Genotyping of hepatitis C virus by melting curve analysis with SYBR Green I

2004 ◽  
Vol 41 (2) ◽  
pp. 130-132 ◽  
Author(s):  
H. Fujigaki ◽  
M. Takemura ◽  
K. Takahashi ◽  
Y. Yamada ◽  
H. Fujii ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Sybr Green I ◽  
Melting Curve Analysis
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