Isolation and Characterization of Phosphatidylinositol 4-Kinase Isoenzymes from Bovine Brain

Author(s):  
G. Vereb ◽  
M. Schmidt ◽  
D. Klix ◽  
M. Varsányi ◽  
L. M. G. Heilmeyer
2003 ◽  
pp. 99-105
Author(s):  
Edon Melloni ◽  
Mauro Michetti ◽  
Franca Salamino ◽  
Roberto Minafra ◽  
Bianca Sparatore ◽  
...  

1986 ◽  
Vol 11 (6) ◽  
pp. 851-867 ◽  
Author(s):  
John D. Bradley ◽  
John N. Whitaker

Author(s):  
Albrecht Moritz ◽  
Jan Westerman ◽  
Pierre N.E. De Graan ◽  
Bernard Payrastre ◽  
Willem H. Gispen ◽  
...  

1979 ◽  
Vol 32 (2) ◽  
pp. 325-333 ◽  
Author(s):  
John N. Whitaker ◽  
Jerome M. Seyer

1988 ◽  
Vol 13 (4) ◽  
pp. 349-357 ◽  
Author(s):  
Vicent Casad� ◽  
Josefa Mallol ◽  
Jorge Bozal

1993 ◽  
Vol 21 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Shin-ichiro Kidou ◽  
Masaaki Umeda ◽  
Atsushi Kato ◽  
Hirofumi Uchimiya

1992 ◽  
Vol 288 (2) ◽  
pp. 637-642 ◽  
Author(s):  
N Divecha ◽  
C E L Brooksbank ◽  
R F Irvine

We detail the purification and characterization of three distinct isoforms of PtdIns4P 5-kinase present in bovine brain. One of these, PtdIns4P 5-kinase C, was purified to apparent homogeneity, and SDS/PAGE analysis demonstrated a single polypeptide and molecular mass 53 KDa. These three isoforms were shown to differ in their kinetic properties, and immunological characterization with an antibody raised to PtdIns4P 5-kinase C demonstrated that this isoform was unrelated to the other two. Furthermore, PtdIns4P 5-kinase C was shown to be the bovine brain homologue of the Type II PtdIns4P 5-kinase previously purified from human erythrocytes [Bazenet, Ruano, Brockman & Anderson (1990) J. Biol. Chem. 265, 18012-18022].


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