Fluorescence-Activated Chromosome Sorting

1992 ◽  
pp. 168-176
Author(s):  
J. A. Fantes ◽  
D. K. Green
Keyword(s):  
Chromosome Sorting

Flow Cytometric Analysis and Chromosome Sorting of Barley (Hordeum vulgare L.)

2000 ◽  
Vol 10 (6) ◽  
pp. 619-625
Author(s):  
Jai-Heon Lee ◽  
K. Arumuganathan ◽  
Young-Soo Chung ◽  
Kee-Young Kim ◽  
Won-Bok Chung ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Flow Cytometric Analysis ◽  
Chromosome Sorting ◽  
Hordeum Vulgare L ◽  
Flow Cytometric

Applications of combined DNA microarray and chromosome sorting technologies

2004 ◽  
Vol 12 (1) ◽  
pp. 35-43 ◽  
Author(s):  
S. M. Gribble ◽  
H. Fiegler ◽  
D. C. Burford ◽  
E. Prigmore ◽  
F. Yang ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Chromosome Sorting

[19] Chromosome sorting by flow cytometry

1987 ◽  
pp. 252-267 ◽  
Author(s):  
Marty Bartholdi ◽  
Julie Meyne ◽  
Kevin Albright ◽  
Mary Luedemann ◽  
Evelyn Campbell ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Sorting

scholarly journals Human Gene Mapping and Chromosome Sorting by Laser Technology.

1993 ◽  
Vol 42 (4) ◽  
pp. 212-216
Author(s):  
Nobuyoshi Shimizu ◽  
Shinsei Minoshima ◽  
Jun Kudoh ◽  
Kazuhiko Kawasaki ◽  
Yimin Wang ◽  
...  
Keyword(s):  
Gene Mapping ◽  
Human Gene ◽  
Chromosome Sorting ◽  
Laser Technology ◽  
Human Gene Mapping

High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11

Science ◽  
1984 ◽  
Vol 225 (4657) ◽  
pp. 57-59 ◽  
Author(s):  
R. Lebo ◽  
F Gorin ◽  
R. Fletterick ◽  
F. Kao ◽  
M. Cheung ◽  
...  
Keyword(s):  
High Resolution ◽  
Blot Analysis ◽  
Chromosome 11 ◽  
Chromosome Sorting ◽  
Mcardle's Syndrome

Cell synchronization and isolation of metaphase chromosomes from maize (Zea mays L.) root tips for flow cytometric analysis and sorting

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 697-703 ◽  
Author(s):  
Jai-Heon Lee ◽  
K. Arumuganathan ◽  
S. M. Kaeppler ◽  
C. M. Papa ◽  
H. F. Kaeppler
Keyword(s):  
Cell Cycle ◽  
Flow Cytometric Analysis ◽  
Maize Root ◽  
Root Tip ◽  
Root Tips ◽  
Chromosome Sorting ◽  
Synthesis Inhibitor ◽  
Metaphase Chromosomes ◽  
Flow Karyotype ◽  
Flow Cytometric

Accumulation of cells containing metaphase chromosomes is an important step in cytological analyses and chromosome sorting procedures. The goal of this research was to optimize treatment parameters to synchronize the cell cycle of maize root tip meristem cells. Levels of hydroxyurea, a DNA synthesis inhibitor, were assessed for their utility in accumulating cells at the G1 phase of the cell cycle. Trifluralin, amiprophos-methyl, and colchicine were used to accumulate cells containing metaphase chromosomes upon release from hydroxyurea inhibition. Optimal mitotic indices were achieved by treating seedlings with 5 mM hydroxyurea for 18 h, incubating for 1 h without chemical treatment to release the hydroxyurea block, and then treating emerging roots with 1 μM trifluralin for 4 h. The mitotic index of synchronized maize root tips was over 70%. Uniformity of synchronization depended upon selection of seeds with emerging radicles that were similar in length at the time of treatment. Suspensions of intact chromosomes were prepared by a simple slicing procedure. The chromosome preparations were found to be suitable for flow cytometric characterization and sorting. Chromosome peaks of the observed flow karyotype resembled the predicted flow karyotype calculated on the basis of maize chromosome size. Key words : flow karyotype, hydroxyurea, plant chromosome sorting, trifluralin.

scholarly journals Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!

2014 ◽  
Vol 26 (4) ◽  
pp. 1448-1463 ◽  
Author(s):  
Laurie Grandont ◽  
Nieves Cuñado ◽  
Olivier Coriton ◽  
Virgine Huteau ◽  
Frédérique Eber ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Meiotic Recombination ◽  
Homoeologous Chromosome ◽  
Chromosome Sorting
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