Micropropagation of Date Palm Using Inflorescence Explants

One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley.

Download Full-text

Indirect Somatic Embryogenesis from Mature Inflorescence Explants of Date Palm

Methods in Molecular Biology - Date Palm Biotechnology Protocols Volume I ◽

10.1007/978-1-4939-7156-5_8 ◽

2017 ◽

pp. 89-97

Author(s):

Ali M. Al-Ali ◽

Chien-Ying Ko ◽

Sultan A. Al-Sulaiman ◽

Sami O. Al-Otaibi ◽

Abd Ulmoneem H. Al-Khamees ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Date Palm ◽

Indirect Somatic Embryogenesis ◽

Inflorescence Explants

Download Full-text

Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00115-4 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Rania A. Taha ◽

Mai A. Allam ◽

S. A. M. Hassan ◽

Basem M. M. Bakr ◽

Mona M. Hassan

Keyword(s):

Growth Regulators ◽

Date Palm ◽

Direct Organogenesis ◽

Naphthaleneacetic Acid ◽

Multiplication Rate ◽

Immature Inflorescence ◽

Plant Materials ◽

Indole Butyric Acid ◽

Promising Tool ◽

Inflorescence Explants

Abstract Background Inflorescence explants of date palm proved to be a promising tool for micropropagation of elite cultivars or rare males and females as organogenesis and somatic embryogenesis could be achieved. These plant materials are abundantly available every year and can be used as cheap and potent explants. Nevertheless, many difficulties could be faced in this protocol according to selection of the spathe size and age, media components, growth regulators, etc. The aim of this study was to determine the influence of various cytokinins on direct organs induction of three date palm cultivars (Selmi, Barhee, and Medjool) from immature inflorescence. An additional objective of this study was to investigate the effect of cytokinins and auxins on growth and development of Medjool cultivar. Results Various combinations of cytokinins were investigated on three date palm inflorescences as N6-(2-isopentenyl) adenine (2iP), kinetin, benzyleadenine (BA), and thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-yl urea) (TDZ). TDZ alone or in combination with BA proved to be superior for direct organogenesis in all three cultivars so that another combination of TDZ with BA was conducted. Results showed that moderate concentration of BA, with TDZ, gave superior response. Medjool cultivar response surpassed other two cultivars that made the possibility to conduct some growth regulators treatments on its multiplication and regeneration. TDZ at 0.5 + BA at 1.0 mg/l without activated charcoal seemed to enhance multiplication rate. Medium containing 0.5 mg/l of both naphthaleneacetic acid and indole butyric acid in addition to 1.0 mg/l indole acetic acid appeared to be more suitable for rooting stage of Medjool shootlets. Conclusion In this study, we created an innovation sequence of growth regulators included in nutrient media for date palm direct organogenesis from inflorescence. Organogenesis has been accelerated from immature inflorescence explants and developed to healthy plantlets which acclimatized in greenhouse.

Download Full-text