scholarly journals Proliferation of female inflorescence explants of date palm

2012 ◽  
Vol 57 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Rehab A. Sidky ◽  
M.M. Eldawyati
Keyword(s):  
Date Palm ◽  
Female Inflorescence ◽  
Inflorescence Explants

scholarly journals In vitro response of date palm (Phoenix dactylifera L.) inflorescence explants to high 2iP and 2,4-D concentrations

2020 ◽  
pp. 831-835
Author(s):  
Juliana Martins Ribeiro ◽  
Silvio Lopes Teixeira ◽  
Joselita Cardoso de Souza ◽  
Brenda Lima Ribeiro ◽  
Antônio Bruno Nunes Oliveira ◽  
...  
Keyword(s):  
Culture Medium ◽  
Date Palm ◽  
Root Formation ◽  
Phoenix Dactylifera ◽  
Culture Technique ◽  
Phoenix Dactylifera L ◽  
Inflorescence Explants ◽  
In Vitro Response ◽  
Floral Bud

One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, explants did not have swelling, callus or root formation. The treatment containing 150 mg/L 2,4-D in the presence of 1.5 mg/L 2iP initiated explant swelling, and treatments with either 100 mg/L or 150 mg/L 2,4-D, combined with 3.0 mg/L 2iP, were also efficient in stimulating in vitro swelling of inflorescence buds. Rhizogenesis was induced at the highest concentrations of 2,4-D (100 and 150 mg/L), combined with 4.5 mg/L 2iP, and was visually more evident in the treatment containing 150 mg/L 2,4-D + 4.5 mg/L 2iP. These results suggest that even higher concentrations of these two reagents might be efficient in the micropropagation of new existing date palm genotypes in the Submedium São Francisco River Valley.

Indirect Somatic Embryogenesis from Mature Inflorescence Explants of Date Palm

2017 ◽  
pp. 89-97
Author(s):  
Ali M. Al-Ali ◽  
Chien-Ying Ko ◽  
Sultan A. Al-Sulaiman ◽  
Sami O. Al-Otaibi ◽  
Abd Ulmoneem H. Al-Khamees ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Date Palm ◽  
Indirect Somatic Embryogenesis ◽  
Inflorescence Explants

scholarly journals Thidiazuron-induced direct organogenesis from immature inflorescence of three date palm cultivars

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Rania A. Taha ◽  
Mai A. Allam ◽  
S. A. M. Hassan ◽  
Basem M. M. Bakr ◽  
Mona M. Hassan
Keyword(s):  
Growth Regulators ◽  
Date Palm ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Immature Inflorescence ◽  
Plant Materials ◽  
Indole Butyric Acid ◽  
Promising Tool ◽  
Inflorescence Explants

Abstract Background Inflorescence explants of date palm proved to be a promising tool for micropropagation of elite cultivars or rare males and females as organogenesis and somatic embryogenesis could be achieved. These plant materials are abundantly available every year and can be used as cheap and potent explants. Nevertheless, many difficulties could be faced in this protocol according to selection of the spathe size and age, media components, growth regulators, etc. The aim of this study was to determine the influence of various cytokinins on direct organs induction of three date palm cultivars (Selmi, Barhee, and Medjool) from immature inflorescence. An additional objective of this study was to investigate the effect of cytokinins and auxins on growth and development of Medjool cultivar. Results Various combinations of cytokinins were investigated on three date palm inflorescences as N6-(2-isopentenyl) adenine (2iP), kinetin, benzyleadenine (BA), and thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-yl urea) (TDZ). TDZ alone or in combination with BA proved to be superior for direct organogenesis in all three cultivars so that another combination of TDZ with BA was conducted. Results showed that moderate concentration of BA, with TDZ, gave superior response. Medjool cultivar response surpassed other two cultivars that made the possibility to conduct some growth regulators treatments on its multiplication and regeneration. TDZ at 0.5 + BA at 1.0 mg/l without activated charcoal seemed to enhance multiplication rate. Medium containing 0.5 mg/l of both naphthaleneacetic acid and indole butyric acid in addition to 1.0 mg/l indole acetic acid appeared to be more suitable for rooting stage of Medjool shootlets. Conclusion In this study, we created an innovation sequence of growth regulators included in nutrient media for date palm direct organogenesis from inflorescence. Organogenesis has been accelerated from immature inflorescence explants and developed to healthy plantlets which acclimatized in greenhouse.

scholarly journals Plant Regeneration from Mature Inflorescence of Date Palm by Using Moringa Extract

2020 ◽  
pp. 1-11
Author(s):  
Eman M. M. Zayed ◽  
S. S. Darwesh Rasmia ◽  
Khaled Abd-El Hamed
Keyword(s):  
Amino Acids ◽  
Plant Regeneration ◽  
Moringa Oleifera ◽  
Date Palm ◽  
Callus Formation ◽  
Mature Female ◽  
Average Percentage ◽  
Female Inflorescence ◽  
Moringa Oleifera Leaves

Plant tissue culture is becoming an important technique for the massive propagation of date palm and the available source lead to the success of this process. The mature female inflorescence is a novel and available source for in vitro propagation of date palm, nowadays become needed at the large scale to usage of organic nature materials in agriculture practice which are rich in plant growth regulators as well as micro elements. This study describes in vitro plant regeneration from mature female inflorescence explants of evaluating date palm (Phoenix dactylifera L.) by using the methanol extract of Moringa oleifera leaves which has great potential to be used as a natural source of nutraceutical in micropropagation. Mature inflorescences explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of an extract of  Moringa leaves with or without add TDZ at 0.2 and 0.6 BA mg/l. Indoles, phenols,  proteins,  amino acids, and carbohydrates were determined. The addition of different concentrations of an extract of Moringa oleifera leaves with TDZ at 0.2 and 0.6 BA mg/l gave the highest significant average percentage of callus formation, friable callus, germination of embryos and numbers of embryos comparing with other treatments. Also, given the highest significant values of free amino acids and carbohydrates were noted in callus and embryo germination stage.

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