Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro

Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots. Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilization time on the viability of honeysuckle explants at the stage of culture introduction in vitro. The effect of the growth regulator Cytodef in the QL nutrient medium on organogenesis was studied at the stage of micropropagation proper, the effect of auxin IBA on plant root formation – at the stage of rooting in vitro. Results and discussion. The greatest viability of honeysuckle explants (80–94%) was registered in the samples affected by Lizoformin 3000 (5%) and silver nitrate (0.2%) as sterilizing agents with a sterilization time of 10 min at the stage of in vitro culture introduction. The biggest quantity (8.8 pcs.) and total length (40.1 cm) of microshoots were observed when the content of cytokinin Cytodef in the culture medium QL was 0.3 mg/L at the stage micropropagation proper. The Boreal Beast cultivar had the largest total length of shoots (29.0 cm). The biggest quantity (5.5 pcs.) and total length (30.8 cm) of roots resulted from 0.5 mg/L of auxin IBA at the stage of rooting in vitro. Coconut substrate produced the highest survival rate (92–99%) at the stage of adaptation to non-sterile conditions in vivo, with the greatest number of leaves (8.1–10.2 pcs.) observed in Canadian cultivars. Conclusion. Cytodef and IBA proved to be effective growth-regulating substances for microplants of Russian and Canadian honeysuckle cultivars in vitro, which makes them promising for berry plantations.

Ecophysiological properties of a cluster root-forming plant, Helicia cochinchinensis (Proteaceae) grown on Miyajima Island, Japan

Aims The family Proteaceae is one of the dominant families in nutrient-impoverished habitats in the Southern hemisphere, and less common in the Northern hemisphere. Helicia cochinchinensis Lour. is the only Proteaceae species in Japan. This study aimed to unveil the ecophysiological properties of H. cochinchinensis grown on Miyajima Island, Hiroshima, Japan.Methods Phosphorus (P) status and dynamics of soils in H. cochinchinensis habitats were measured. Plant P and nitrogen (N) concentrations of leaves were measured after digestion. Roots and rhizosheath soil were collected to assess root morphology and root exudates.Results Available P (Olsen-P) in soils in habitats of H. cochinchinensis was 0.46–3.7 mg P kg-1 soil. Citrate was the major carboxylate in root exudates and its concentration increased during cluster-root formation. Acid phosphatase activity was greater at the surface of cluster roots that on the surface of other roots and bulk soil, especially for mature cluster roots. Sparingly soluble organic P concentrations decreased in the rhizosheath soil of mature cluster roots. The P concentrations of H. cochinchinensis leaves were relatively low; 0.34–0.69 mg P g-1 DW and 0.15–0.29 mg P g-1 DW in mature and senesced leaves, respectively. The P demand of H. cochinchinensis was less than that of nearby trees, showing greater P-remobilization efficiency.Conclusions Phosphorus mobilization from unavailable P by cluster roots supported P uptake by H. cochinchinensis, and P remobilization from senescing leaves contributed to sustain growth under P-deficient conditions.

Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

Cryopreservation of Arachis Hypogaea L. Varieties, from the INIAP-Ecuador Germplasm Bank

The peanut (Arachis hypogaea L.) is recognized as one of the most important legume crops globally for its use in human food; it is widely distributed and cultivated in tropical and subtropical regions. The purpose of this study was to evaluate the cryopreservation of five peanut varieties conserved in the INIAP Germplasm Bank, testing cryopreservation methods, evaluating the germination percentage of whole seeds and embryonic shoots. Subsequently, two quantitative variables, shoot length and root, were evaluated. The average germination percentage of varieties and treatments was higher when embryonic axes were isolated with 99.31% than 86.06% seeds. The best germination percentage of the five varieties for seeds and embryonic shoots was obtained by the Peruvian variety with 88.13% and 92.50%. The best treatments by variety for the germination of whole seeds and embryonic axes were obtained by the treatment (desiccation and NL) for whole seeds (GS2) with 95.42% and embryonic axes with 92.83%. Ageing and cryopreservation treatments positively affected germination and seedling vigor in whole seeds and embryonic axes. The two quantitative variables, shoot and root length showed variability between the five varieties; significant differences were observed between the four treatments evaluated for whole seeds and embryonic axes. The three treatments for whole seeds (GS1, GS2 GS3) and the non-cryopreserved control treatment (GSC), as well as the treatments for embryonic axes (GEA1, GEA2 GEA3) and the non-cryopreserved control treatment (GEAC), obtained good survival. They germinate whole seeds and embryonic axes with sprout development (aerial part) and root formation. With the most effective treatments for whole seeds (GS2) and embryonic axes (GEA2), the cryopreservation of the national peanut collection of the INIAP Germplasm Bank could be started.

Influence of growth regulators on yield of carrot tablets in the conditions of the forest-steppe of right-bank Ukraine

In the results conducted between studies, the studied regulators affect the biometrics and yield of table carrot plants. The greatest influence on the interphase period "root formation - technical maturity" was performed by the regulator Phytocid-r, which helped reduce the growth of the interphase period Shantane KL by 3 days, in the hybrid Brilliance F1 by 4 days. With the use of the growth regulator Phytocid-r of the vegetation period of the population in the variety Shantane KL – 117 days, in the hybrid Brilliance F1 – 118 days, respectively 6 and 7 days shorter with control. The highest weight of root crops during the growing season was observed using the growth regulator Phytocid-p, in the variety Shantane KL the increase relative to control was 15.5 – 23.7 g, in the hybrid Brilliance F1 – 18.5 and 25.5 g. root crops were also characterized by variants using growth regulators Ivin and Emistym C, where the increase relative to the control at the end of the growing season was: in the variety Shantane KL – 4.1-8.3 g, in the hybrid Brilliance F1 – 4.9 and 6.0 g respectively. The growing season of table carrots was characterized by slightly higher temperatures and low rainfall, with a long dry period, which negatively affected the formation of roots of table carrots. However, the highest yield was obtained from the option using the growth regulator Phytocid-p, which provided an increase in yield at the level: in the variety Shantane KL – 8.5 t / ha, in the hybrid Brilliance F1 – 13.3 t / ha. The highest percentage of marketable yield was obtained using the growth regulator Phytocid-p in both varieties and hybrids – 94.8 and 95.2 %, which is more than the control by 11.2 and 11.0 %. The largest mass of root crop was formed by plants using the growth regulator Phytocid-r: in the variety Shantane KL – 112 g, in the hybrid Brilliance F1 – 118 g, which is 23.8 and 25.6 g more than the control. The diameter of the root was in the range of 5.8-7.0 cm and varied slightly from the growth regulator. The hybrid Brilliance F1 was characterized by a longer root length. However, taking into account the applied growth regulator, it was found that the highest figure was for the use of Phytocid-r: in the variety Shantane KL – 17.6 cm, in the hybrid Brilliance F1 – 18.3 cm, which is more than the option without treatment 1.2 and 1.0 cm.

In Vitro Regeneration of Miscanthus x giganteus through Indirect Organogenesis: Effect of Explant Type and Growth Regulators

Miscanthus x giganteus is a spontaneous sterile hybrid therefore the creation of useful genetic diversity by conventional breeding methods is restricted. Plant regeneration through indirect organogenesis may be a useful approach to create genetic variability of this important agricultural crop. The present study aimed to evaluate the effect of the explant type and growth regulators on indirect organogenesis of Miscanthus x giganteus and to determine the ploidy level of plant regenerants by flow cytometry. On average, the highest percentage of morphogenic callus tested explants formed in the medium supplemented with 2.5 mg L–1 IBA + 0.1 mg L–1 BAP + 4.0 mg L–1 l-proline. The most intensive secondary differentiation of callus cells was observed in the medium supplemented with 4.0 mg L–1 ZEA + 1.0 mg L–1 NAA. The highest root formation frequency with the highest number of roots was determined in the MS nutrient medium supplemented with 0.4 mg L–1 IBA, where more than 95% of plant regenerants survived and were growing normally.

Displacement and Arrest of Root Formation in a Permanent Maxillary Central Incisor Subsequent to Trauma to the Primary Predecessor: A Case Report

The present report aimed to explore the case of an 8-year-old patient with chief complaint of the lack of eruption of the maxillary right permanent central incisor, referring to the Department of Pediatric Dentistry, Faculty of Dentistry, Hamadan University of Medical Sciences. The corresponding tooth on the contralateral side had fully erupted. The patient’s history revealed that the predecessor deciduous tooth had sustained a trauma, resulting in the partial intrusion of the tooth into the alveolar bone, that is, the relative intrusion of the deciduous central incisor. CBCT examinations were ordered for further evaluation, which showed the upward displacement of the permanent tooth bud in the alveolar bone as a result of the trauma, adhering to the floor of the nasal cavity. Therefore, root formation was halted, making the tooth embedded.

Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

The unique and balanced components of the biochemical composition, together with high antioxidant activity, make the red beet necessary a dietary vegetable crop, much contributing to healthy food ration. The application of the technology for producing gynogenic plants in vitro increases the genetic diversity and significantly reduces the period of time required to obtain the appropriate homozygous lines used to create the F1 hybrids that are demanded in the market. For induction of gynogenesis, we used IMB medium developed by us with the addition of 55 g/L sucrose, 3 g/L phytogel, 200 mg/L ampicillin, and 0.4 mg/L thidiazuron (TDZ) and cultured at 28 °C in the dark for 4–6 weeks. Shoot regeneration from embryoids and callus was performed on MS medium with 20 g/L sucrose, 3 g/L phytogel, 1 mg/L 6-benzylaminopurine (BAP), and 0.1 mg/L gibberellic acid (GA3). Immersion of the obtained microshoots with 5–7 well-developed leaves for 10–15 s into concentrated sterile indole-3-butyric acid (IBA) solution (50 mg/L) followed by their cultivation on solid medium ½ IMB with 2% sucrose and 3 g/L phytogel was the most efficient method for root formation. The addition of silver nitrate (22 mg/L) to the nutrient medium provoked an increase in the number of induced ovules up to nine per Petri dish (up to 25% of induced ovules). Gynogenic development was produced in six out of 11 genotypes studied, and the plants that were then acclimatized to ex vitro conditions were obtained in three genotypes (Nezhnost’, Dobrynya, b/a 128). The evaluation of ploidy of gynogenic plants that was carried out by flow cytometry and direct counting of chromosomes stained with propion-lacmoide revealed that all obtained gynogenic plants were haploids (2n = x = 9).

INFLUENCE OF PHYSIOLOGICALLY ACTIVE SUBSTANCES ON DEVELOPMENT OF OWN-ROOTED SEEDLINGS IN NURSERY

The purpose of the research was to identify growth stimulators that promote the activa-tion of growth processes and root formation, to improve the technology of growing high-quality own-rooted grape seedlings from shortened three-eyed cuttings. The influence of physiologically active substances (PAS): Epin, Silk, Kornevin, Nikfan, Kavkaz on sur-vival, activation of growth processes, root formation of shortened cuttings of Bianca grapes was determined. As a result of pro-cessing cuttings with PAS, the survival rate of cuttings in the nursery increased by 10.6–19.1 % relative to the control. The yield of seedlings of the variety in the experimental variants exceeded the control indicators by 2.8–18.4 %. The effect of preparations on plant growth was noted, the increase in one-year growth in the variants varied from 5.8 to 18.0 cm relative to the control. When the ba-sal part of the cuttings was briefly immersed in a 0.01 % solution of Nikfan, the growth length was 78.1 cm, when soaked in a 0.1 % solution of Kornevin – 76.8 cm, in a 0.1 % solution of Silk –75.5 cm, in the control this indicator was 60.1 cm. The positive effect of growth stimulants on the development of the root system has been established. The indica-tor of the average number of roots per own-rooted seedling for the variants varied from 10.5 to 12.5 pcs., in the control variant – 10.7 pcs. According to the complex of indi-cators, a variant with the treatment of the ba-sal part of the cuttings for 24 hours with the preparation Kornevin-0.1% was identified.