Surgical Technique of Heterotopic Heart Transplantation

1990 ◽  
pp. 81-87
Author(s):  
D. Novitzky ◽  
D. K. C. Cooper
Keyword(s):  
Surgical Technique ◽  
Heart Transplantation ◽  
Heterotopic Heart Transplantation

The Surgical Technique of Heterotopic Heart Transplantation

1983 ◽  
Vol 36 (4) ◽  
pp. 476-482 ◽  
Author(s):  
D. Novitzky ◽  
D.K.C. Cooper ◽  
C.N. Barnard
Keyword(s):  
Surgical Technique ◽  
Heart Transplantation ◽  
Heterotopic Heart Transplantation

Development of a Vascularized Heterotopic Neonatal Rat Heart Transplantation Model

2016 ◽  
Vol 57 (3-4) ◽  
pp. 240-251
Author(s):  
Shogo Shimada ◽  
Pedro J. del Nido ◽  
Ingeborg Friehs
Keyword(s):  
Surgical Technique ◽  
Heart Transplantation ◽  
Rat Heart ◽  
Surgical Techniques ◽  
Operation Time ◽  
Neonatal Rat ◽  
Venous Anastomosis ◽  
Ischemic Time ◽  
Heterotopic Heart Transplantation ◽  
Neonatal Rat Heart

Background/Purpose: Rodent adult-to-adult heterotopic heart transplantation is a well-established animal model, and the detailed surgical technique with several modifications has been previously described. In immature donor organ transplantation, however, the surgical technique needs to be revised given the smaller size and fragility of the donor graft. Here, we report our surgical technique for heterotopic abdominal (AHTx) and femoral (FHTx) neonatal rat heart transplantation based on an experience of over 300 cases. Methods: Heterotopic heart transplantation was conducted in syngeneic Lewis rats. Neonatal rats (postnatal day 2-4) served as donors. AHTx was performed by utilizing the conventional adult-to-adult transplant method with specific modifications for optimal aortotomy and venous anastomosis. In the FHTx, the donor heart was vascularized by connecting the donor's aorta and pulmonary artery to the recipient's right femoral artery and vein, respectively, in an end-to-end manner. A specifically fashioned butterfly-shaped rubber sheet was used to align the target vessels properly. The transplanted graft was visually assessed for its viability and was accepted as a technical success when the viability met specific criteria. Successfully transplanted grafts were subject to further postoperative evaluation. Forty cases (AHTx and FHTx; n = 20 each) were compared regarding perioperative parameters and outcomes. Results: Both models were technically feasible (success rate: AHTx 75% vs. FHTx 70%) by refining the conventional heterotopic transplant technique. Injury to the fragile donor aorta and congestion of the graft due to suboptimal venous connection were predominant causes of failure, leading to refractory bleeding and poor graft viability. Although the FHTx required significantly longer operation time and graft ischemic time, the in situ graft viabilities were comparable. The FHTx provided better postoperative monitoring as it enabled daily graft palpation and better echocardiographic visualization. Conclusions: We describe detailed surgical techniques for AHTx and FHTx while addressing neonatal donor-specific issues. Following our recommendations potentially reduces the learning curve to achieve reliable and reproducible results with these challenging animal models.

Heterotopic Heart Transplantation in Rats: Improved Anesthetic and Surgical Technique

2010 ◽  
Vol 42 (9) ◽  
pp. 3828-3832 ◽  
Author(s):  
A. Ruzza ◽  
R. Vespignani ◽  
L.S. Czer ◽  
M. De Robertis ◽  
G.N. Wu ◽  
...  
Keyword(s):  
Surgical Technique ◽  
Heart Transplantation ◽  
Heterotopic Heart Transplantation

Long term outcome after heterotopic heart transplantation

2011 ◽  
Vol 59 (S 01) ◽  
Author(s):  
J Sindermann ◽  
I Alejnik ◽  
J Hoffmann ◽  
S Klotz ◽  
A Löher ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Heterotopic Heart Transplantation

scholarly journals Two hearts, one soul: heterotopic heart transplantation follow-up with cardiac computed tomography

2016 ◽  
Vol 37 (44) ◽  
pp. 3356-3356 ◽  
Author(s):  
Charles Roux ◽  
Shaida Varnous ◽  
Pascal Leprince ◽  
Philippe Cluzel
Keyword(s):  
Computed Tomography ◽  
Heart Transplantation ◽  
Cardiac Computed Tomography ◽  
Heterotopic Heart Transplantation

Effects of paced counterpulsation on exercise capacity and hemodynamics after heterotopic heart transplantation

1995 ◽  
Vol 75 (5) ◽  
pp. 415-417 ◽  
Author(s):  
Richard P.W. Cowell ◽  
Jayne Morris-Thurgood ◽  
John G. Coghlan ◽  
Charles D.J. Ilsley ◽  
Andrew G. Mitchell ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Exercise Capacity ◽  
Heterotopic Heart Transplantation

scholarly journals Magnetic resonance imaging in heterotopic heart transplantation

1989 ◽  
Vol 98 (3) ◽  
pp. 457-459
Author(s):  
Albert de Roos ◽  
Joost Doornbos ◽  
Albert V.G. Bruschke ◽  
Ad. E. van Voorthuisen ◽  
Magdi Yacoub
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Heart Transplantation ◽  
Resonance Imaging ◽  
Heterotopic Heart Transplantation

scholarly journals Use of peroxiredoxin for preconditioning of heterotopic heart transplantation in a rat

2020 ◽  
Vol 22 (2) ◽  
pp. 158-164
Author(s):  
N. V. Grudinin ◽  
V. K. Bogdanov ◽  
M. G. Sharapov ◽  
N. S. Bunenkov ◽  
N. P. Mozheiko ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Reperfusion Injury ◽  
Mammalian Cells ◽  
Ischemia Reperfusion Injury ◽  
Isolated Heart ◽  
Ischemia Reperfusion ◽  
Signal Transmission ◽  
Redox Homeostasis ◽  
Heterotopic Heart Transplantation ◽  
Wide Range

Peroxiredoxin 6 (Prdx6) is an antioxidant enzyme in the human body that performs a number of important functions in the cell. Prdx6 restores a wide range of peroxide substrates, thus playing a leading role in maintaining redox homeostasis in mammalian cells. In addition to peroxidase activity, Prdx6 has an activity of phospholipase A2, thus taking part in membrane phospholipid metabolism. Due to its peroxidase and phospholipase activity, Prdx6 participates in intracellular and intercellular signal transmission, thereby facilitating the initiation of regenerative processes in the cell, suppression of apoptosis and activation of cell proliferation. Given the functions performed, Prdx6 can effectively deal with oxidative stress caused by various factors, including ischemia-reperfusion injury. On an animal model of rat heterotopic heart transplantation, we showed the cardioprotective potential of exogenous recombinant Prdx6, introduced before transplantation and subsequent reperfusion injury of the heart. It has been demonstrated that exogenous Prdx6 effectively alleviates the severity of ischemia-reperfusion injury of the heart by 2–3 times, providing normalization of its structural and functional state during heterotopic transplantation. The use of recombinant Prdx6 can be an effective approach in preventing/alleviating ischemia-reperfusion injury of the heart, as well as in maintaining an isolated heart during transplantation.

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