From Time-Resolved Difference Spectra to Kinetics, Mechanism, and Thermodynamics in the Bacteriorhodopsin Photocycle

1992 ◽  
pp. 85-96 ◽  
Author(s):  
J. K. Lanyi
Keyword(s):  
Difference Spectra ◽  
Time Resolved ◽  
Bacteriorhodopsin Photocycle

Time-Resolved Step-Scan FT-IR Investigations of the Transition from KL to L in the Bacteriorhodopsin Photocycle: Identification of Chromophore Twists by Assigning Hydrogen-Out-Of-Plane (HOOP) Bending Vibrations

1993 ◽  
Vol 47 (9) ◽  
pp. 1394-1400 ◽  
Author(s):  
Olaf Weidlich ◽  
Friedrich Siebert
Keyword(s):  
Single Bond ◽  
Difference Spectra ◽  
Bending Vibrations ◽  
Time Resolved ◽  
Molecular Events ◽  
Out Of Plane ◽  
Ft Ir ◽  
Bacteriorhodopsin Photocycle ◽  
Protein Part ◽  
Ft Ir Spectroscopy

Sub-microsecond time-resolved step-scan FT-IR spectroscopy is applied to the study of the molecular changes and their dynamics occurring during the KL-L transition of bacteriorhodopsin. The time-resolved difference spectra are compared to the static low-temperature BR → K and BR → L difference spectra. Our data show that the protein part in KL is similar to that in K. However, the chromophore is more relaxed and is differently twisted. A strong hydrogen-out-of-plane (HOOP) mode in KL is assigned to the 15-HOOP. As is the case for L, a strong deformation of the C14-C15 single bond is deduced for KL. Evidence of a KL → L equilibrium is presented. In N, a 15-HOOP mode similar to that in L is observed, indicating very similar twists of the C14-C15 single bond. This observation excludes major contributions of this deformation to the reduction of the pKa of the Schiff base in L. From the spectral changes, important molecular events are deduced that occur in the transitions to KL, L, and N.

scholarly journals Nanosecond time-resolved infrared spectroscopy distinguishes two K species in the bacteriorhodopsin photocycle

1995 ◽  
Vol 68 (5) ◽  
pp. 2073-2080 ◽  
Author(s):  
J. Sasaki ◽  
T. Yuzawa ◽  
H. Kandori ◽  
A. Maeda ◽  
H. Hamaguchi
Keyword(s):  
Infrared Spectroscopy ◽  
Time Resolved ◽  
Time Resolved Infrared Spectroscopy ◽  
Bacteriorhodopsin Photocycle

scholarly journals Time-Resolved Infrared Spectroscopy of Binuclear Rhenium (I) Polypyridyl Complexes in Solution

1999 ◽  
Vol 19 (1-4) ◽  
pp. 279-282 ◽  
Author(s):  
L. C. Abbott ◽  
C. J. Arnold ◽  
K. C. Gordon ◽  
R. E. Hester ◽  
J. N. Moore ◽  
...  
Keyword(s):  
Mixed Valence ◽  
Difference Spectra ◽  
Kinetic Measurements ◽  
Time Resolved ◽  
Bridging Ligand ◽  
Ligand Charge Transfer ◽  
Complex Forms ◽  
Time Resolved Infrared Spectroscopy ◽  
State Kinetic ◽  
Mlct State

A series of four binuclear rhenium (I) complexes of the general form [Re(CO)3Cl]2BL, where BL is a polypyridyl bridging ligand, have been studied using ultrafast time-resolved UV/visible (TRVIS) and infrared (TRIR) spectroscopies. Visible excitation produces a metal-to-ligand charge-transfer (MLCT) excited state. Kinetic measurements show that the lifetime of this MLCT state varies between 100 and 1900 ps, depending on the structure of the bridging ligand. TRIR difference spectra show that each complex forms a similar MLCT state which has mixed valence character.

scholarly journals Time-resolved absorption and fluorescence from the bacteriorhodopsin photocycle in the nanosecond time regime

1993 ◽  
Vol 64 (5) ◽  
pp. 1512-1519 ◽  
Author(s):  
J.K. Delaney ◽  
T.L. Brack ◽  
G.H. Atkinson
Keyword(s):  
Time Resolved ◽  
Time Regime ◽  
Bacteriorhodopsin Photocycle

LED array spectrophotometer for measurement of time resolved difference spectra in the 530?600 nm wavelength region

1990 ◽  
Vol 25 (3) ◽  
pp. 317-327 ◽  
Author(s):  
Christof Klughammer ◽  
J�rg Kolbowski ◽  
Ulrich Schreiber
Keyword(s):  
Wavelength Region ◽  
Difference Spectra ◽  
Time Resolved ◽  
Led Array
Sign in / Sign up

Export Citation Format

Share Document