Functional Sub-states by High-pressure Macromolecular Crystallography

2015 ◽  
pp. 215-235 ◽  
Author(s):  
Anne-Claire Dhaussy ◽  
Eric Girard
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography

scholarly journals New methodologies at PF AR-NW12A: the implementation of high-pressure macromolecular crystallography

2013 ◽  
Vol 20 (6) ◽  
pp. 838-842 ◽  
Author(s):  
Leonard Michel Gabriel Chavas ◽  
Tadayuki Nagae ◽  
Hiroyuki Yamada ◽  
Nobuhisa Watanabe ◽  
Yusuke Yamada ◽  
...  
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography ◽  
New Methodologies

A new dimension in structural biology: fully fledged high-pressure macromolecular crystallography

2004 ◽  
Vol 24 (1) ◽  
pp. 173-182 ◽  
Author(s):  
Eric Girard ◽  
Richard Kahn ◽  
Isabella Ascone ◽  
Mohamed Mezouar ◽  
Anne-Claire Dhaussy ◽  
...  
Keyword(s):  
High Pressure ◽  
Structural Biology ◽  
Macromolecular Crystallography

High-pressure macromolecular crystallography and NMR: status, achievements and prospects

2012 ◽  
Vol 22 (5) ◽  
pp. 636-642 ◽  
Author(s):  
Roger Fourme ◽  
Eric Girard ◽  
Kazuyuki Akasaka
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography

Using a quasi-parallel X-ray beam of ultrashort wavelength for high-pressure virus crystallography: implications for standard macromolecular crystallography

2003 ◽  
Vol 59 (10) ◽  
pp. 1767-1772 ◽  
Author(s):  
Roger Fourme ◽  
Éric Girard ◽  
Richard Kahn ◽  
Isabella Ascone ◽  
Mohamed Mezouar ◽  
...  
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography ◽  
X Ray

Macromolecular crystallography at high pressure with pneumatic diamond anvil cells handled by a six-axis robotic arm

2010 ◽  
Vol 43 (4) ◽  
pp. 762-768 ◽  
Author(s):  
Eric Girard ◽  
Roger Fourme ◽  
Romain Ciurko ◽  
Jacques Joly ◽  
Florian Bouis ◽  
...  
Keyword(s):  
High Pressure ◽  
Robotic Arm ◽  
Data Sets ◽  
Macromolecular Crystallography ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Diamond Anvil ◽  
Hen Egg White ◽  
Lysozyme Crystals ◽  
Oscillation Method

A new pneumatic diamond anvil cell has been constructed, generating continuous pressure and temperature variations in the range 0–2.5 GPa and 293–393 K. The cell is designed mainly for high-pressure macromolecular crystallography and should facilitate pressure and temperature annealing of the sample. The analysis is reported of several diffraction data sets of tetragonal hen egg-white lysozyme crystals loaded either in the new cell or in a currently used membrane-based cell. These experiments were performed on beamline FIP-BM30A at the ESRF, Grenoble, a macromolecular crystallography beamline on a bending magnet. Cells were handled and automatically centred by a six-axis robotic arm that was used as a goniometer for data collection by the oscillation method.

Advances in High-Pressure Biophysics: Status and Prospects of Macromolecular Crystallography

2009 ◽  
Vol 38 (1) ◽  
pp. 153-171 ◽  
Author(s):  
Roger Fourme ◽  
Eric Girard ◽  
Richard Kahn ◽  
Anne-Claire Dhaussy ◽  
Isabella Ascone
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography

scholarly journals A high-pressure macromolecular crystallography capability developed at CHESS

2020 ◽  
Vol 76 (a1) ◽  
pp. a172-a172
Author(s):  
Xin Huang ◽  
Zhongwu Wang ◽  
Aaron Finke ◽  
Doletha Szebenyi ◽  
Qingqiu Huang ◽  
...  
Keyword(s):  
High Pressure ◽  
Macromolecular Crystallography

Towards a high-throughput system for high-pressure cooling of cryoprotectant-free biological crystals

2014 ◽  
Vol 47 (2) ◽  
pp. 584-592 ◽  
Author(s):  
Peter van der Linden ◽  
Fabien Dobias ◽  
Hugo Vitoux ◽  
Ulrike Kapp ◽  
Jeroen Jacobs ◽  
...  
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Noble Gas ◽  
Macromolecular Crystallography ◽  
Structural Modifications ◽  
Amorphous Ice ◽  
Aqueous Solvent ◽  
Technical Details ◽  
Synchrotron Beamlines ◽  
Cooling Procedure

A prototype of a high-pressure cooling apparatus dedicated to macromolecular crystallography on synchrotrons is reported. The system allows cooling of biological crystals without the addition of penetrating or nonpenetrating exogenous cryoprotectant by transforming the aqueous solvent into high-density amorphous ice at a pressure of 200 MPa. The samples are directly fished from crystallization trays with cryopins specifically designed for the pressurizing device and which are compatible with robotized sample changers on synchrotron beamlines. Optionally, the system allows noble gas derivatization during the high-pressure cooling procedure. Some technical details of the equipment and of the method are described in this article. A representative series of test crystals shows that the system is capable of successfully cooling samples that normally require a wide variety of cryoprotection conditions. The last section focuses on pressure-induced structural modifications of these proteins, which are shown to be few but nevertheless of interest.

High pressure macromolecular crystallography: The 140-MPa crystal structure at 2.3 Å resolution of urate oxidase, a 135-kDa tetrameric assembly

2006 ◽  
Vol 1764 (3) ◽  
pp. 391-397 ◽  
Author(s):  
Nathalie Colloc'h ◽  
Eric Girard ◽  
Anne-Claire Dhaussy ◽  
Richard Kahn ◽  
Isabella Ascone ◽  
...  
Keyword(s):  
Crystal Structure ◽  
High Pressure ◽  
Urate Oxidase ◽  
Macromolecular Crystallography ◽  
Tetrameric Assembly
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