Development and characterization of a generalized gene tagging system for higher plants using an engineered maize transposon Ac

1992 ◽  
Vol 20 (2) ◽  
pp. 177-198 ◽  
Author(s):  
Wayne P. Fitzmaurice ◽  
Lori J. Lehman ◽  
Long V. Nguyen ◽  
William F. Thompson ◽  
Earl A. Wernsman ◽  
...  
Keyword(s):  
Higher Plants ◽  
Gene Tagging ◽  
Tagging System

Functional characterization of chloroplast-targeted RbgA GTPase in higher plants

2017 ◽  
Vol 95 (4-5) ◽  
pp. 463-479 ◽  
Author(s):  
Young Jeon ◽  
Hee-Kyung Ahn ◽  
Yong Won Kang ◽  
Hyun-Sook Pai
Keyword(s):  
Higher Plants ◽  
Functional Characterization

scholarly journals In Vitro Import of a Nuclearly Encoded tRNA into Mitochondria of Solanum tuberosum

2003 ◽  
Vol 23 (11) ◽  
pp. 4000-4012 ◽  
Author(s):  
Ludovic Delage ◽  
André Dietrich ◽  
Anne Cosset ◽  
Laurence Maréchal-Drouard
Keyword(s):  
Solanum Tuberosum ◽  
Higher Plants ◽  
Plant Mitochondria ◽  
Protein Translation ◽  
Trna Genes ◽  
Vitro Assay ◽  
Trna Import

ABSTRACT Some of the mitochondrial tRNAs of higher plants are nuclearly encoded and imported into mitochondria. The import of tRNAs encoded in the nucleus has been shown to be essential for proper protein translation within mitochondria of a variety of organisms. Here, we report the development of an in vitro assay for import of nuclearly encoded tRNAs into plant mitochondria. This in vitro system utilizes isolated mitochondria from Solanum tuberosum and synthetic tRNAs transcribed from cloned nuclear tRNA genes. Although incubation of radioactively labeled in vitro-transcribed tRNAAla, tRNAPhe, and tRNAMet-e with isolated potato mitochondria resulted in importation, as measured by nuclease protection, the amount of tRNA transcripts protected at saturation was at least five times higher for tRNAAla than for the two other tRNAs. This difference in in vitro saturation levels of import is consistent with the in vivo localization of these tRNAs, since cytosolic tRNAAla is naturally imported into potato mitochondria whereas tRNAPhe and tRNAMet-e are not. Characterization of in vitro tRNA import requirements indicates that mitochondrial tRNA import proceeds in the absence of any added cytosolic protein fraction, involves at least one protein component on the surface of mitochondria, and requires ATP-dependent step(s) and a membrane potential.

Antimicrobial Agents from Higher Plants. The Isolation and Structural Characterization of Two Additional Pterocarpan Antimicrobial Agents from Nigerian Erythrina mildbraedii

Heterocycles ◽  
1988 ◽  
Vol 27 (11) ◽  
pp. 2517 ◽  
Author(s):  
Lester A. Mitscher ◽  
Simon K. Okwute ◽  
Sitaraghav R. Gollapudi ◽  
Ali Keshavarz-Shokri
Keyword(s):  
Structural Characterization ◽  
Antimicrobial Agents ◽  
Higher Plants

LOP1: a gene involved in auxin transport and vascular patterning in Arabidopsis

Development ◽  
1996 ◽  
Vol 122 (6) ◽  
pp. 1811-1819 ◽  
Author(s):  
F.M. Carland ◽  
N.A. McHale
Keyword(s):  
Cell Expansion ◽  
Higher Plants ◽  
Lateral Roots ◽  
Primary Root ◽  
Vascular Patterning ◽  
Basipetal Transport ◽  
Leaf Mutant ◽  
Identification And Characterization ◽  
Axial Growth

We have taken a genetic approach to understanding the mechanisms that control vascular patterning in the leaves of higher plants. Here we present the identification and characterization of the lop1 mutant of Arabidopsis which is defective in basipetal transport of IAA. Mutant leaf midveins show disoriented axial growth, and bifurcation into twin veins that are frequently rotated out of the normal dorsal/ventral axis of the leaf. Mutant plants also display abnormal patterns of cell expansion in the midrib cortex and in the epidermis of the elongation zone of lateral roots. Lateral roots show abnormal curvature during initiation, sometimes encircling the primary root prior to growth in a normal downward direction. Mutant seedlings have normal levels of free IAA, and appear normal in auxin perception, suggesting that transport is the primary lesion. The abnormalities in vascular development, lateral root initiation and patterns of cell expansion observed in the lop] mutant are consistent with a basic disruption in basipetal transport of IAA.

Isolation and Characterization of Ice-Binding Proteins from Higher Plants

2014 ◽  
pp. 255-277 ◽  
Author(s):  
Adam J. Middleton ◽  
Barbara Vanderbeld ◽  
Melissa Bredow ◽  
Heather Tomalty ◽  
Peter L. Davies ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Higher Plants ◽  
Isolation And Characterization ◽  
Ice Binding
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