Inheritance of resistance and genetic relationships among soybean plant introductions to races of soybean cyst nematode

The soybean cyst nematode (SCN) Heterodera glycines is the most economically important pathogen of soybean, capable of causing large scale yield loss on a global scale. Current management practices utilize host resistance, but commercial resistance is limited to three main sources, the soybean plant introductions (PI) 54802 (Peking), 88788, and 437654. More than 95% of soybean grown in the north central United States have resistance derived from PI 88788 and repeated use of this resistance has led to the development of highly virulent nematode populations capable of infecting these resistant plants and causing yield losses. In order to identify the mode of inheritance for virulence on the three primary sources of resistance to SCN a controlled crossing study was performed, mating a highly virulent nematode (TN20) with an avirulent nematode (PA3). By monitoring the offspring of this cross it was found that depending on the source of host resistance, virulence is inherited in a dominant or a recessive manner except for virulence on PI 437654, which is likely a multigenic trait dependent on multiple recessive genes. A de novo transcriptome assembly was then generated for SCN and mined for novel stylet-secreted effectors, identifying a new pool of candidates that may play a role in virulence. Finally, a comparative transcriptomic analysis was performed across multiple SCN populations to identify conserved expression patterns and genes associated with virulence. Results from these studies will be used to improve current management practices for SCN and provide new potential targets for improving SCN resistance.

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Reaction of Soybean Cyst Nematode Resistant Plant Introductions to Root-Knot and Reniform Nematodes

Plant Breeding and Biotechnology ◽

10.9787/pbb.2015.3.4.346 ◽

2015 ◽

Vol 3 (4) ◽

pp. 346-354 ◽

Cited By ~ 4

Author(s):

Jeong-Dong Lee ◽

Hyun-Jee Kim ◽

Robert T. Robbins ◽

James A. Wrather ◽

Jason Bond ◽

...

Keyword(s):

Soybean Cyst Nematode ◽

Cyst Nematode ◽

Resistant Plant ◽

Plant Introductions ◽

Reniform Nematodes

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Classification methods and identification of reniform nematode resistance in known soybean cyst nematode resistant soybean genotypes

Plant Disease ◽

10.1094/pdis-01-21-0051-re ◽

2021 ◽

Author(s):

Mariola Usovsky ◽

Robert Robbins ◽

Juliet Fultz Wilkes ◽

Devany Crippen ◽

Vijay Shankar ◽

...

Keyword(s):

Soybean Cyst Nematode ◽

Germplasm Collection ◽

Nematode Species ◽

The United States ◽

Cyst Nematode ◽

Classification Methods ◽

Reniform Nematode ◽

Plant Introductions ◽

Scn Resistance ◽

Moderately Resistant

Plant parasitic nematodes are a major yield-limiting factor of soybean in the United States and Canada. It has been indicated that soybean cyst nematode (SCN, Heterodera glycines Ichinohe) and reniform nematode (RN, Rotylenchulus reniformis Linford and Oliveira) resistance could be genetically related. For many years fragmentary data has shown this relationship. This report evaluates RN reproduction on 418 plant introductions (PIs) selected from the USDA Soybean Germplasm Collection with reported SCN resistance. The germplasm was divided into two tests of 214 PIs reported as resistant, and 204 PIs moderately resistant to SCN. The defining and reporting of RN resistance changed several times in the last 30 years, causing inconsistencies in RN resistance classification among multiple experiments. Comparison of four RN resistance classification methods was performed: (1) ≤10% as compared to the susceptible check, (2) using normalized reproduction index (RI) values, and transformed data (3) log10 (x) and (4) log10 (x+1), in an optimal univariate k-means clustering analysis. The method of transformed data log10 (x) was selected as the most accurate for classification of RN resistance. Among 418 PIs with reported SCN resistance, the log10 (x) method grouped 59 PIs (15%) as resistant, and 130 PIs (31%) as moderately resistant to RN. Genotyping of a subset of the most resistant PIs to both nematode species revealed their strong correlation with rhg1-a allele. This research identified genotypes with resistance to two nematode species and potential new sources of RN resistance that could be valuable to breeders in developing resistant cultivars.

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Genetic Relationships among Soybean Plant Introductions with Resistance to Soybean Cyst Nematodes

Crop Science ◽

10.2135/cropsci1997.0011183x003700060048x ◽

1997 ◽

Vol 37 (6) ◽

pp. 1966-1972 ◽

Cited By ~ 32

Author(s):

B. W. Diers ◽

H. T. Skorupska ◽

A. P. Rao‐Arelli ◽

S. R. Cianzio

Keyword(s):

Genetic Relationships ◽

Soybean Plant ◽

Cyst Nematodes ◽

Plant Introductions ◽

Soybean Cyst Nematodes

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Accessions of Perennial Glycine Species With Resistance to Multiple Types of Soybean Cyst Nematode (Heterodera glycines)

Plant Disease ◽

10.1094/pdis-10-16-1472-re ◽

2017 ◽

Vol 101 (7) ◽

pp. 1201-1206 ◽

Cited By ~ 5

Author(s):

L. Wen ◽

C. Yuan ◽

T. K. Herman ◽

G. L. Hartman

Keyword(s):

Resistance Genes ◽

Soybean Cyst Nematode ◽

Heterodera Glycines ◽

Cost Effective ◽

Cyst Nematode ◽

Disease Genes ◽

Sources Of Resistance ◽

Plant Introductions ◽

Glycine Species ◽

Cost Effective Method

Soybean cyst nematode (SCN; Heterodera glycines; HG) is a widely occurring and damaging pathogen that limits soybean production. Developing resistant cultivars is the most cost-effective method for managing this disease. Genes conferring SCN resistance in soybean have been identified; however, there are SCN populations that overcome known resistance genes. In order to identify additional sources of resistance and potentially new resistance genes, 223 plant introductions (PIs) of G. tomentella and 59 PIs of 12 other perennial Glycine species were inoculated with HG Types 0, HG 2, and HG 1.2.3, and then 36 PIs out of this set were further evaluated with HG Type 1.2.3.4.5.6.7, a population that overcomes all the resistance genes in soybean. Of 223 G. tomentella PIs evaluated, 86 were classified as resistant to three HG types, 69 as resistant to two HG types, and 22 as resistant to one HG type. Of the other 12 perennial Glycine species, all PIs of G. argyrea and G. pescadrensis were resistant to all three HG types. Of the 36 PIs challenged with HG Type 1.2.3.4.5.6.7, 35 were resistant with 16 showing no cyst reproduction. Our study confirms that there are high levels of resistance to SCN among the perennial Glycine species. This represents an untapped resource for use in genetic studies and for improving resistance to SCN in soybean.

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Using Independent Culling to Screen Plant Introductions for Combined Resistance to Soybean Cyst Nematode and Sudden Death Syndrome

Crop Science ◽

10.2135/cropsci2005.12.0505 ◽

2006 ◽

Vol 46 (5) ◽

pp. 2081-2083 ◽

Cited By ~ 5

Author(s):

J. R. Gelin ◽

P. R. Arelli ◽

G. A. Rojas‐Cifuentes

Keyword(s):

Sudden Death ◽

Soybean Cyst Nematode ◽

Cyst Nematode ◽

Sudden Death Syndrome ◽

Plant Introductions ◽

Independent Culling

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Inheritance of resistance to soybean cyst nematode races 3 and 14 in soybean RIL and F2 populations

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2007001200010 ◽

2007 ◽

Vol 42 (12) ◽

pp. 1735-1740 ◽

Cited By ~ 1

Author(s):

Marcia Flores da Silva ◽

Ivan Schuster ◽

Gerardo Domingo Lucio Cervigni ◽

João Flávio Veloso da Silva ◽

Waldir Pereira Dias ◽

...

Keyword(s):

Soybean Cyst Nematode ◽

Recombinant Inbred Lines ◽

Recessive Gene ◽

Inbred Lines ◽

Cyst Nematode ◽

Inheritance Of Resistance ◽

Number Of Genes ◽

Complete Resistance ◽

Recessive Genes ◽

Moderate Resistance

The objective of this work was to evaluate the soybean inheritance of resistance to cyst nematode races 3 and 14. The following populations where evaluated: one population of recombinant inbred lines (RILs) [Hartwig (resistant) x Y23 (susceptible line)] for races 3, 14 and 9; one population of families F2:3 [M-SOY 8001 (resistant) x MB/BR 46 - Conquista (susceptible)] for race 3; and one population of families F2:3 [(S5995 (resistant) x BRSMG Renascença (susceptible)] for race 14. In RIL populations, four epistatic genes were identified which conditioned resistance to race 14, and three epistatic ones for resistance to races 3 and 9. The lack of one gene provided moderate resistance under all situations. The highest number of genes for resistance to race 14 points out that genes responsible for lower effects might be involved. In population F2:3 from M-SOY 8001 x MB/BR 46 - Conquista, one recessive gene for moderate resistance and two recessive genes complete resistance to race 3 were identified. Two recessive genes conditioning moderate resistance to race 14 were identified in population F2:3 from the crossing S5995 x BRSMG Renascença. These results will be useful in designing crossings, involving these parentals, with higher possibility to accumulating genes that provide resistance to several SCN races.

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