Molecular characterization of the cinnabar region of Drosophila melanogaster: Identification of the cinnabar transcription unit

Genetica ◽  
1996 ◽  
Vol 98 (3) ◽  
pp. 249-262 ◽  
Author(s):  
William D. Warren ◽  
Stephanie Palmer ◽  
Antony J. Howells
Biochemistry ◽  
1993 ◽  
Vol 32 (23) ◽  
pp. 6011-6018 ◽  
Author(s):  
James J. Murtagh ◽  
Fang Jen S. Lee ◽  
Peter Deak ◽  
Linda M. Hall ◽  
Lucia Monaco ◽  
...  

Genetics ◽  
1991 ◽  
Vol 128 (2) ◽  
pp. 373-380
Author(s):  
D H Teng ◽  
L B Bender ◽  
C M Engele ◽  
S Tsubota ◽  
T Venkatesh

Abstract The complementary lethal interaction between the prune (pn) and Killer of prune loci of Drosophila melanogaster is an unusual and highly specific phenomenon. A lesion in pn results in a brownish-purple color of the compound eyes, while the conditional dominant Killer of prune mutation exhibits no phenotype by itself. However, a hemizygous or homozygous pn mutant carrying a copy of the Killer of prune gene dies during the late second to third instar stage of larval development. As a step toward understanding the molecular nature of this lethality and the role of pn in pigment biosynthesis, we have cloned the pn locus by using a transposon tag in the P element-induced allele, pn38. In addition, seven independent revertant lines were generated by the remobilization of transposons in pn38. The pn gene is located in a region that is transcriptionally active, and the isolated cDNAs that correspond to this area fall into three transcription units: I, II and III. Southern analysis shows that the restriction fragment length polymorphisms in five pn alleles are localized within a 1.2-kilobase genomic fragment, of which only transcription unit II is a part. The cDNA of this unit recognizes 1.65- and 1.8-kilobase messages in wild-type Drosophila adult head and body tissues that are absent or extremely reduced in pn mutants. Taken together, the results suggest that transcription unit II defines a part of the pn locus and its cDNA encodes a putative structural gene of pn.


Genetics ◽  
2006 ◽  
Vol 174 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Gunjan H. Arya ◽  
Matthew J. P. Lodico ◽  
Omar I. Ahmad ◽  
Rohul Amin ◽  
John E. Tomkiel

2012 ◽  
Vol 11 (93) ◽  
pp. 15967-15974 ◽  
Author(s):  
Maji Priyanka ◽  
Ch ◽  
Chakrabarti rasekhar ◽  
Chatterjee Soumendranath

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