An 'Intermetallic' Molecular Nanomagnet with the Lanthanide Coordinated Only by Transition Metals

The best performing molecular nanomagnets are currently designed by carefully arranging p-element donor atoms (usually carbon, nitrogen and/or oxygen) around the central magnetic ion. Inspired by the structure of the hardest intermetallic magnet SmCo5, we have demonstrated a nanomagnetic molecule where the central lanthanide (Ln) ion Er is coordinated solely by three transition metal (TM) ions in a perfectly trigonal planar fashion. The molecule [Er(ReCp2)3] (ErRe3) constitutes the first example of a molecular nanomagnet (MNM; or single molecule magnet SMM) with unsupported Ln-TM bonds and paves the way towards molecular intermetallics with strong direct magnetic exchange interactions. Such interactions are believed to be crucial for quenching the quantum tunneling of magnetization which limits the application of Ln-SMMs as sub-nanometer magnetic memory units.

Elevated P-Element-Induced Wimpy-Testis-Like Protein 1 Expression Predicts Unfavorable Prognosis for Patients with Various Cancers

Increasing evidence has shown that overexpression of P-element-induced wimpy-testis (PIWI)-like protein 1 (PIWIL1) was associated with unfavorable prognosis of patients with various types of cancers. Herein, we conducted this meta-analysis to identify the clinicopathological and prognostic value of the PIWIL1 expression in cancers. Three electronic databases (PubMed, Web of Science, and Embase) were comprehensively retrieved for relevant studies up to August 4th, 2019. RevMan 5.3 and STATA 12.0 statistical software programs were used to explore the relationships between PIWIL1 expression and the prognosis and clinicopathological features in cancer patients. A total of 13 studies recruiting 2179 patients with 9 types of solid tumors were finally included in the meta-analysis. The results indicated that patients with high PIWIL1 expression tended to have a shorter survival, and additionally deeper tumor invasion, higher clinical stage, and more lymph node metastasis. PIWIL1 could serve as a biomarker for prognosis and clinicopathological characteristics in various cancers.

INDY—From Flies to Worms, Mice, Rats, Non-Human Primates, and Humans

I’m Not Dead Yet (Indy) is a fly homologue of the mammalian SLC13A5 (mSLC13A5) plasma membrane citrate transporter, a key metabolic regulator and energy sensor involved in health, longevity, and disease. Reduction of Indy gene activity in flies, and its homologs in worms, modulates metabolism and extends longevity. The metabolic changes are similar to what is obtained with caloric restriction (dietary restriction). Similar effects on metabolism have been observed in mice and rats. As a citrate transporter, INDY regulates cytoplasmic citrate levels. Indy flies heterozygous for a P-element insertion have increased spontaneous physical activity, increased fecundity, reduced insulin signaling, increased mitochondrial biogenesis, preserved intestinal stem cell homeostasis, lower lipid levels, and increased stress resistance. Mammalian Indy knockout (mIndy-KO) mice have higher sensitivity to insulin signaling, lower blood pressure and heart rate, preserved memory and are protected from the negative effects of a high-fat diet and some of the negative effects of aging. Reducing mIndy expression in human hepatocarcinoma cells has recently been shown to inhibit cell proliferation. Reduced Indy expression in the fly intestine affects intestinal stem cell proliferation, and has recently been shown to also inhibit germ cell proliferation in males with delayed sperm maturation and decreased spermatocyte numbers. These results highlight a new connection between energy metabolism and cell proliferation. The overrall picture in a variety of species points to a conserved role of INDY for metabolism and health. This is illustrated by an association of high mIndy gene expression with non-alcoholic fatty liver disease in obese humans. mIndy (mSLC13A5) coding region mutations (e.g., loss-of-function) are also associated with adverse effects in humans, such as autosomal recessive early infantile epileptic encephalopathy and Kohlschütter−Tönz syndrome. The recent findings illustrate the importance of mIndy gene for human health and disease. Furthermore, recent work on small-molecule regulators of INDY highlights the promise of INDY-based treatments for ameliorating disease and promoting healthy aging.

Spatial and temporal requirement of Mlp60A isoforms during muscle development and function in Drosophila melanogaster

Many myofibrillar proteins undergo isoform switching in a spatio-temporal manner during muscle development. The biological significance of the variants of several of these myofibrillar proteins remains elusive. One such myofibrillar protein, the Muscle LIM Protein (MLP), is a vital component of the Z-discs. In this paper, we show that one of the Drosophila MLP encoding genes, Mlp60A, gives rise to two isoforms: a short (279 bp, 10 kDa) and a long (1461 bp, 54 kDa) one. The short isoform is expressed throughout development, but the long isoform is adult-specific, being the dominant of the two isoforms in the indirect flight muscles (IFMs). A concomitant, muscle-specific knockdown of both isoforms leads to late pupal lethality, with the surviving flies being majorly flight defective. Mlp60A null flies show developmental lethality, and muscle defects in the individuals surviving till the third instar larval stage. This lethality could be rescued partially by muscle-specific overexpression of the short isoform. Almost 90% of the long isoform-specific P-element insertion mutant flies show a compromised flight ability and have reduced sarcomere length. Hence, our data shows that the two Mlp60A isoforms are functionally specialized, to ensuring normal embryonic muscle development and adult flight muscle function.

1D modelling and PID control of helicopter Diesel engine rotational speed in torque changes

The article discusses the results of simulation tests concerning the operation of a diesel engine for a light helicopter. The tests were carried out in the AVL Boost software which is used to analyze dynamic phenomena in internal combustion engines. The research object was a newly designed diesel engine of a V8 structure and a power of 330 kW. This engine was designed to be used in the construction of a light class helicopter. The created one-dimensional simulation model included all the main engine components as well as the connection to the helicopter main transmission and the helicopter rotor. The tests consisted in selecting the P value in the PID controller used to control the amount of fuel injected into the engine. The change in the P value indirectly influenced the reaction of the engine to a change in power and torque during horizontal flight of a helicopter. These changes were introduced by changing thrust torque in the helicopter rotor. The fuel injection regulator was designed to maintain a constant engine rotational speed. The maximum speed deviations from the nominal speed of the engine operation due to both increasing and decreasing speed were analyzed. Additionally, the sum of the deviation values was analyzed until the rotational speed of the tested object stabilized. The results showed that the change of the P parameter affects all the analyzed parameters of the engine operation; however, the minimum deviation values for each parameter occur at non-equal PID settings, which makes it difficult to clearly indicate the appropriate value of the P element.

NILs of Cold Tolerant Japonica Cultivar Exhibited New QTLs for Mineral Elements in Rice

Chilling stress at booting stage can cause floret deterioration and sterility by limiting the supply of food chain and the accumulation of essential mineral elements resulting in reduction of yield and grain quality attributes in rice. Genomic selection of chilling tolerant rice with reference to the accumulation of mineral elements will have great potential to cope with malnutrition and food security in times of climate change. Therefore, a study was conducted to explore the genomic determinants of cold tolerance and mineral elements content in near-isogenic lines (NILs) of japonica rice subjected to chilling stress at flowering stage. Detailed morphological analysis followed by quantitative analysis of 17 mineral elements revealed that the content of phosphorus (P, 3,253 mg/kg) and potassium (K, 2,485 mg/kg) were highest while strontium (Sr, 0.26 mg/kg) and boron (B, 0.34 mg/kg) were lowest among the mineral elements. The correlation analysis revealed extremely positive correlation of phosphorus (P) and copper (Cu) with most of the cold tolerance traits. Among all the effective ear and the second leaf length correlation was significant with half of the mineral elements. As a result of comparative analysis, some QTLs (qBRCC-1, qBRCIC-2, qBRZC-6, qBRCHC-6, qBRMC-6, qBRCIC-6a, qBRCIC-6b, qBRCHC-6, and qBRMC-6) identified for calcium (Ca), zinc (Zn), chromium (Cr) and magnesium (Mg) on chromosome number 1, 2, and 6 while, a novel QTL (qBCPC-1) was identified on chromosome number 1 for P element only. These findings provided bases for the identification of candidate genes involved in mineral accumulation and cold tolerance in rice at booting stage.

Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation

ABSTRACT Adult stem cells are critical for the maintenance of tissue homeostasis. However, how the proliferation and differentiation of intestinal stem cells (ISCs) are regulated remains not fully understood. Here, we find a mutant, stum 9-3, affecting the proliferation and differentiation of Drosophila adult ISCs in a forward genetic screen for factors regulating the proliferation and differentiation ISCs. stum 9-3 acts through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor. Interestingly, the phenotype of stum 9-3 mutant is not caused by disruption of stumble (stum), where the p-element is inserted. Detailed mapping, rescue experiments and mutant characterization show that stum 9-3 is a new allele of O-fucosyltransferase 1 (O-fut1). Our results indicate that unexpected mutants with interesting phenotype could be recovered in forward genetic screens using known p-element insertion stocks.

MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells

The PIWI (P-element-induced wimpy testis)-interacting-RNA (piRNA) pathway plays a crucial role in the repression of TE (transposable element) expression via de novo DNA methylation in mouse embryonic male germ cells. Various proteins, including MIWI2 are involved in the process. TE silencing is ensured by piRNA-guided MIWI2 that recruits some effector proteins of the DNA methylation machinery to TE regions. However, the molecular mechanism underlying the methylation is complex and has not been fully elucidated. Here, we identified MORC3 as a novel associating partner of MIWI2 and also a nuclear effector of retrotransposon silencing via piRNA-dependent de novo DNA methylation in embryonic testis. Moreover, we show that MORC3 is important for transcription of piRNA precursors and subsequently affects piRNA production. Thus, we provide the first mechanistic insights into the role of this effector protein in the first stage of piRNA biogenesis in embryonic TE silencing mechanism.

ANALISIS EFISIENSI PRODUKSI DAN STRUKTUR BIAYA PADA USAHATANI NANAS DI DESA ASTOMULYO KECAMATAN PUNGGUR KABUPATEN LAMPUNG TENGAH

This study purposes are to analyze factors that affect production of pineapple farming, production efficiency of pineapple farming, and cost structure of pineapple farming. Data were collected at Astomulyo Village Punggur Subdistrict Central Lampung Regency in December 2019 – February 2020. The number of respondents was 63 farmers consisting of 58 farmers who were members of farmer groups and the rest were non-members of farmer groups by using simple random sampling. The primary data were obtained from direct interview with the pineapple farmers and the secondary data were obtained from several related studies and institutions. The data were analyzed using Stochastic Frontier production function. The study shows the factors affecting the production of pineapple farming are land area, C/N ratio, N element, and P element. The pineapple farming is technically, allocatively, and economically efficient yet. The biggest costs on pineapple farming are costs of seed, land lease, and labor.Key words: costs, efficiency, farmers, pineapple