Interhomologue sequence variation of alpha satellite DNA from human chromosome 17: Evidence for concerted evolution along haplotypic lineages

1995 ◽  
Vol 41 (6) ◽  
Author(s):  
PeterE. Warburton ◽  
HuntingtonF. Willard
Keyword(s):  
Human Chromosome ◽  
Concerted Evolution ◽  
Satellite Dna ◽  
Sequence Variation ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna ◽  
Human Chromosome 17

scholarly journals Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement.

1990 ◽  
Vol 10 (12) ◽  
pp. 6374-6380 ◽  
Author(s):  
R Wevrick ◽  
W C Earnshaw ◽  
P N Howard-Peebles ◽  
H F Willard
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Centromeric Region ◽  
Chromosome Rearrangement ◽  
Specific Antigen ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Protein Antigens ◽  
Alpha Satellite Dna ◽  
Human Chromosome 17

A familial, constitutionally rearranged human chromosome 17 is deleted for much of the DNA in its centromeric region but retains full mitotic centromere activity. Fluorescence in situ hybridization, pulsed-field gel electrophoresis, and Southern blot analysis of the residual centromeric region revealed a approximately 700-kb centromeric array of tandemly repeated alpha satellite DNA that was only approximately 20 to 30% as large as a normal array. This deletion was associated with a reduction in the amount of the centromere-specific antigen CENP-B detected by indirect immunofluorescence. The coincidence of the primary constriction, the small residual array of alpha satellite DNA, and the reduced amount of detectable CENP-B support the hypothesis that CENP-B is associated with alpha satellite DNA. Furthermore, the finding that both the deleted chromosome 17 and its derivative supernumerary fragment retained mitotic function and possess centromeric protein antigens suggests that human centromeres are structurally and functionally repetitive.

Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement

1990 ◽  
Vol 10 (12) ◽  
pp. 6374-6380
Author(s):  
R Wevrick ◽  
W C Earnshaw ◽  
P N Howard-Peebles ◽  
H F Willard
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Centromeric Region ◽  
Chromosome Rearrangement ◽  
Specific Antigen ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Protein Antigens ◽  
Alpha Satellite Dna ◽  
Human Chromosome 17

A familial, constitutionally rearranged human chromosome 17 is deleted for much of the DNA in its centromeric region but retains full mitotic centromere activity. Fluorescence in situ hybridization, pulsed-field gel electrophoresis, and Southern blot analysis of the residual centromeric region revealed a approximately 700-kb centromeric array of tandemly repeated alpha satellite DNA that was only approximately 20 to 30% as large as a normal array. This deletion was associated with a reduction in the amount of the centromere-specific antigen CENP-B detected by indirect immunofluorescence. The coincidence of the primary constriction, the small residual array of alpha satellite DNA, and the reduced amount of detectable CENP-B support the hypothesis that CENP-B is associated with alpha satellite DNA. Furthermore, the finding that both the deleted chromosome 17 and its derivative supernumerary fragment retained mitotic function and possess centromeric protein antigens suggests that human centromeres are structurally and functionally repetitive.

Structure of DNA near long tandem arrays of alpha satellite DNA at the centromere of human chromosome 7

Genomics ◽  
1992 ◽  
Vol 14 (4) ◽  
pp. 912-923 ◽  
Author(s):  
Rachel Wevrick ◽  
Vicki P. Willard ◽  
Huntington F. Willard
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Chromosome 7 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna ◽  
Human Chromosome 7 ◽  
Tandem Arrays

Ordered mapping of three alpha satellite DNA subsets on human chromosome 22

1995 ◽  
Vol 3 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Rachele Antonacci ◽  
Mariano Rocchi ◽  
Nicoletta Archidiacono ◽  
Antonio Baldini
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Chromosome 22 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

Detection of an i(17q) chromosome by fluorescent in situ hybridization with a chromosome 17 alpha satellite DNA probe

1992 ◽  
Vol 62 (2) ◽  
pp. 140-143 ◽  
Author(s):  
Hitoshi Nakagawa ◽  
Johji Inazawa ◽  
Shinichi Misawa ◽  
Shinji Tanaka ◽  
Teruyuki Takashima ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Dna Probe ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

Concerted evolution of primate alpha satellite DNA

1990 ◽  
Vol 216 (3) ◽  
pp. 555-566 ◽  
Author(s):  
Sharon J. Durfy ◽  
Huntington F. Willard
Keyword(s):  
Concerted Evolution ◽  
Satellite Dna ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

Detection of novel centromeric polymorphisms associated with alpha satellite DNA from human chromosome 11

1987 ◽  
Vol 77 (2) ◽  
pp. 151-156 ◽  
Author(s):  
John S. Waye ◽  
Gillian M. Greig ◽  
Huntington F. Willard
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Alpha Satellite ◽  
Chromosome 11 ◽  
Alpha Satellite Dna

Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome

1986 ◽  
Vol 6 (9) ◽  
pp. 3156-3165
Author(s):  
J S Waye ◽  
H F Willard
Keyword(s):  
Human Chromosome ◽  
X Chromosome ◽  
Repeat Unit ◽  
Higher Order ◽  
Chromosome 17 ◽  
Crossing Over ◽  
Human Chromosomes ◽  
Alpha Satellite ◽  
Unequal Crossing Over ◽  
Human Chromosome 17

The centromeric regions of all human chromosomes are characterized by distinct subsets of a diverse tandemly repeated DNA family, alpha satellite. On human chromosome 17, the predominant form of alpha satellite is a 2.7-kilobase-pair higher-order repeat unit consisting of 16 alphoid monomers. We present the complete nucleotide sequence of the 16-monomer repeat, which is present in 500 to 1,000 copies per chromosome 17, as well as that of a less abundant 15-monomer repeat, also from chromosome 17. These repeat units were approximately 98% identical in sequence, differing by the exclusion of precisely 1 monomer from the 15-monomer repeat. Homologous unequal crossing-over is suggested as a probable mechanism by which the different repeat lengths on chromosome 17 were generated, and the putative site of such a recombination event is identified. The monomer organization of the chromosome 17 higher-order repeat unit is based, in part, on tandemly repeated pentamers. A similar pentameric suborganization has been previously demonstrated for alpha satellite of the human X chromosome. Despite the organizational similarities, substantial sequence divergence distinguishes these subsets. Hybridization experiments indicate that the chromosome 17 and X subsets are more similar to each other than to the subsets found on several other human chromosomes. We suggest that the chromosome 17 and X alpha satellite subsets may be related components of a larger alphoid subfamily which have evolved from a common ancestral repeat into the contemporary chromosome-specific subsets.

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