A novel callose synthase from pollen tubes of Nicotiana

Planta ◽  
1993 ◽  
Vol 191 (4) ◽  
Author(s):  
Henriette Schl�pmann ◽  
Antony Bacic ◽  
SteveM. Read
Keyword(s):  
Pollen Tubes ◽  
Callose Synthase

Role of a callose synthase zymogen in regulating wall deposition in pollen tubes of Nicotiana alata Link et Otto

Planta ◽  
1999 ◽  
Vol 208 (4) ◽  
pp. 528-538 ◽  
Author(s):  
Huijuan Li ◽  
Antony Bacic ◽  
Steve M. Read
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Callose Synthase ◽  
Wall Deposition

Membrane fractionation and enrichment of callose synthase from pollen tubes of Nicotiana alata Link et Otto

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 380-388 ◽  
Author(s):  
Adrian Turner ◽  
Antony Bacic ◽  
Philip J. Harris ◽  
Steve M. Read
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Callose Synthase ◽  
Membrane Fractionation

scholarly journals Proteomic and biochemical evidence links the callose synthase in Nicotiana alata pollen tubes to the product of the NaGSL1 gene

2007 ◽  
Vol 52 (1) ◽  
pp. 147-156 ◽  
Author(s):  
Lynette Brownfield ◽  
Kris Ford ◽  
Monika Susanne Doblin ◽  
Ed Newbigin ◽  
Steve Read ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Nicotiana Alata ◽  
Callose Synthase ◽  
Biochemical Evidence

Arabidopsis Callose Synthase Gene GSL8 is Required for Cell Wall Formation and Establishment and Maintenance of Quiescent Center

2014 ◽  
Vol 48 (4) ◽  
pp. 389-397
Author(s):  
Liu Lin ◽  
Quan Xianqing ◽  
Zhao Xiaomei ◽  
Huang Lihua ◽  
Feng Shangcai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Quiescent Center ◽  
Cell Wall Formation ◽  
Callose Synthase ◽  
Wall Formation

scholarly journals Villin controls the formation and enlargement of punctate actin foci in pollen tubes

2020 ◽  
Vol 133 (6) ◽  
pp. jcs237404 ◽  
Author(s):  
Wanying Zhao ◽  
Xiaolu Qu ◽  
Yuhui Zhuang ◽  
Ludi Wang ◽  
Maurice Bosch ◽  
...  
Keyword(s):  
Pollen Tubes

Keeping pollen tubes on track

Nature Plants ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 51-52 ◽  
Author(s):  
Yan Ju ◽  
Sharon A. Kessler
Keyword(s):  
Pollen Tubes

scholarly journals Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

2021 ◽  
Vol 40 (2) ◽  
pp. 205-222
Author(s):  
Monica Scali ◽  
Alessandra Moscatelli ◽  
Luca Bini ◽  
Elisabetta Onelli ◽  
Rita Vignani ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Membrane Trafficking ◽  
Tip Growth ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Structural Features ◽  
Two Dimensional Gel Electrophoresis ◽  
Depth Analysis

AbstractPollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identifyNicotiana tabacumDifferentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

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