Cellular localization of NADH-dependent glutamate-synthase protein in vascular bundles of unexpanded leaf blades and young grains of rice plants

Planta ◽  
1994 ◽  
Vol 193 (3) ◽  
Author(s):  
Toshihiko Hayakawa ◽  
Teiji Nakamura ◽  
Fuyu Hattori ◽  
Tadahiko Mae ◽  
Kunihiko Ojima ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Glutamate Synthase ◽  
Vascular Bundles ◽  
Rice Plants

scholarly journals Organization and Structure of Ferredoxin. Dependent Glutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants.

2003 ◽  
Vol 20 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Toshihiko HAYAKAWA ◽  
Takahiro SAKAI ◽  
Keiki ISHIYAMA ◽  
Naoya HIROSE ◽  
Hiroyuki NAKAJIMA ◽  
...  
Keyword(s):  
Intracellular Localization ◽  
Glutamate Synthase ◽  
Enzyme Protein ◽  
Rice Plants

Cell Specific Expression of Nadh-Dependent Glutamate Synthase in Rice Plants

1998 ◽  
pp. 3023-3026
Author(s):  
Keiki Ishiyama ◽  
Soichi Kojima ◽  
Toshihiko Hayakawa ◽  
Tomoyuki Yamaya
Keyword(s):  
Glutamate Synthase ◽  
Specific Expression ◽  
Rice Plants

Analysis of a promoter for the NADH - glutamate synthase gene in rice (Oryza sativa): cell type-specific expression in developing organs of transgenic rice plants

2000 ◽  
Vol 27 (9) ◽  
pp. 787 ◽  
Author(s):  
Soichi Kojima ◽  
Michiko Kimura ◽  
Yukine Nozaki ◽  
Tomoyuki Yamaya
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Gus Activity ◽  
Start Codon ◽  
Assimilate Transport ◽  
Upstream Region ◽  
Vascular Bundles ◽  
Specific Expression ◽  
Gus Reporter

This paper originates from a presentation at the International Conference on Assimilate Transport and Partitioning, Newcastle, NSW, August 1999 The entire 3.7 kbp 5´-upstream region (–2840 to +886) from the translational start codon of NADH–glutamate synthase (NADH–GOGAT, EC 1.4.1.14) gene from rice (Oryza sativa L.) or the region sequentially deleted from the 5´-end was fused with the β−glucuronidase (GUS) reporter gene. The chimeric gene was introduced into calli derived from rice scutellum via Agrobacterium tumefaciens-mediated transformation and tissue-specific GUS activity determined in T0 generations. When the entire region was fused, GUS activity was detected in vascular bundles of the developing leaf blade and in dorsal and lateral vascular bundles of developing grains. This corresponds with our previous immunodetection of NADH–GOGAT protein (Hayakawa et al., Planta 193, 455–460, 1994). A series of deletion experiments showed that a 149-nucleotide region between –142 and +7 was essential for promoter activity in the NADH–GOGAT gene.

scholarly journals Studies on Morphogenesis in Rice Plants : 7. The morphology of vascular bundles in the vegetative nodes of the culm

1974 ◽  
Vol 43 (3) ◽  
pp. 389-401 ◽  
Author(s):  
Harunosuke KAWAHARA ◽  
Nobuo CHONAN ◽  
Toshiaki MATSUDA
Keyword(s):  
Vascular Bundles ◽  
Rice Plants

scholarly journals Metaproteomic Identification of Diazotrophic Methanotrophs and Their Localization in Root Tissues of Field-Grown Rice Plants

2014 ◽  
Vol 80 (16) ◽  
pp. 5043-5052 ◽  
Author(s):  
Zhihua Bao ◽  
Takashi Okubo ◽  
Kengo Kubota ◽  
Yasuhiro Kasahara ◽  
Hirohito Tsurumaru ◽  
...  
Keyword(s):  
Epidermal Cells ◽  
Vascular Bundles ◽  
Type Ii ◽  
Content Type ◽  
Rice Plants ◽  
Rice Roots ◽  
Microbial Symbiosis ◽  
Catalyzed Reporter Deposition ◽  
Card Fish ◽  
Root Tissues

ABSTRACTIn a previous study by our group, CH4oxidation and N2fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsible for CH4oxidation and N2fixation under the field conditions. Metaproteomic analysis of root-associated bacteria from field-grown rice (Oryza sativaNipponbare) revealed that nitrogenase complex-containing nitrogenase reductase (NifH) and the alpha subunit (NifD) and beta subunit (NifK) of dinitrogenase were mainly derived from type II methanotrophic bacteria of the familyMethylocystaceae, includingMethylosinusspp. Minor nitrogenase proteins such asMethylocella,Bradyrhizobium,Rhodopseudomonas, andAnaeromyxobacterwere also detected. Methane monooxygenase proteins (PmoCBA and MmoXYZCBG) were detected in the same bacterial group of theMethylocystaceae. Because these results indicated thatMethylocystaceaemembers mediate both CH4oxidation and N2fixation, we examined their localization in rice tissues by using catalyzed reporter deposition-fluorescencein situhybridization (CARD-FISH). The methanotrophs were localized around the epidermal cells and vascular cylinder in the root tissues of the field-grown rice plants. Our metaproteomics and CARD-FISH results suggest that CH4oxidation and N2fixation are performed mainly by type II methanotrophs of theMethylocystaceae, includingMethylosinusspp., inhabiting the vascular bundles and epidermal cells of rice roots.

scholarly journals Organ and Cellular Localization of Asparagine Synthetase in Rice Plants

2000 ◽  
Vol 41 (7) ◽  
pp. 874-880 ◽  
Author(s):  
Kentaro Nakano ◽  
Tatsuro Suzuki ◽  
Toshihiko Hayakawa ◽  
Tomoyuki Yamaya
Keyword(s):  
Cellular Localization ◽  
Asparagine Synthetase ◽  
Rice Plants

scholarly journals Quantitative Intercellular Localization of NADH-Dependent Glutamate Synthase Protein in Different Types of Root Cells in Rice Plants

1999 ◽  
Vol 119 (2) ◽  
pp. 409-416 ◽  
Author(s):  
Toshihiko Hayakawa ◽  
Laura Hopkins ◽  
Lucy J. Peat ◽  
Tomoyuki Yamaya ◽  
Alyson K. Tobin
Keyword(s):  
Glutamate Synthase ◽  
Root Cells ◽  
Rice Plants ◽  
Different Types
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