Cell Specific Expression of Nadh-Dependent Glutamate Synthase in Rice Plants

1998 ◽  
pp. 3023-3026
Author(s):  
Keiki Ishiyama ◽  
Soichi Kojima ◽  
Toshihiko Hayakawa ◽  
Tomoyuki Yamaya
Keyword(s):  
Glutamate Synthase ◽  
Specific Expression ◽  
Rice Plants

scholarly journals Organization and Structure of Ferredoxin. Dependent Glutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants.

2003 ◽  
Vol 20 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Toshihiko HAYAKAWA ◽  
Takahiro SAKAI ◽  
Keiki ISHIYAMA ◽  
Naoya HIROSE ◽  
Hiroyuki NAKAJIMA ◽  
...  
Keyword(s):  
Intracellular Localization ◽  
Glutamate Synthase ◽  
Enzyme Protein ◽  
Rice Plants

Cellular localization of NADH-dependent glutamate-synthase protein in vascular bundles of unexpanded leaf blades and young grains of rice plants

Planta ◽  
1994 ◽  
Vol 193 (3) ◽  
Author(s):  
Toshihiko Hayakawa ◽  
Teiji Nakamura ◽  
Fuyu Hattori ◽  
Tadahiko Mae ◽  
Kunihiko Ojima ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Glutamate Synthase ◽  
Vascular Bundles ◽  
Rice Plants

Tissue-specific expression of the rolC promoter of the Ri plasmid in transgenic rice plants

1989 ◽  
Vol 220 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Rikyu Matsuki ◽  
Haruko Onodera ◽  
Taeko Yamauchi ◽  
Hirofumi Uchimiya
Keyword(s):  
Transgenic Rice ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Rice Plants ◽  
Ri Plasmid ◽  
Transgenic Rice Plants ◽  
Rolc Promoter

Marker-free, tissue-specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants

2012 ◽  
Vol 69 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Yongbin Qi ◽  
Lei Chen ◽  
Xiuling He ◽  
Qingsheng Jin ◽  
Xiaoming Zhang ◽  
...  
Keyword(s):  
Insect Resistance ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Rice Plants ◽  
Marker Free

Analysis of a promoter for the NADH - glutamate synthase gene in rice (Oryza sativa): cell type-specific expression in developing organs of transgenic rice plants

2000 ◽  
Vol 27 (9) ◽  
pp. 787 ◽  
Author(s):  
Soichi Kojima ◽  
Michiko Kimura ◽  
Yukine Nozaki ◽  
Tomoyuki Yamaya
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Glutamate Synthase ◽  
Gus Activity ◽  
Start Codon ◽  
Assimilate Transport ◽  
Upstream Region ◽  
Vascular Bundles ◽  
Specific Expression ◽  
Gus Reporter

This paper originates from a presentation at the International Conference on Assimilate Transport and Partitioning, Newcastle, NSW, August 1999 The entire 3.7 kbp 5´-upstream region (–2840 to +886) from the translational start codon of NADH–glutamate synthase (NADH–GOGAT, EC 1.4.1.14) gene from rice (Oryza sativa L.) or the region sequentially deleted from the 5´-end was fused with the β−glucuronidase (GUS) reporter gene. The chimeric gene was introduced into calli derived from rice scutellum via Agrobacterium tumefaciens-mediated transformation and tissue-specific GUS activity determined in T0 generations. When the entire region was fused, GUS activity was detected in vascular bundles of the developing leaf blade and in dorsal and lateral vascular bundles of developing grains. This corresponds with our previous immunodetection of NADH–GOGAT protein (Hayakawa et al., Planta 193, 455–460, 1994). A series of deletion experiments showed that a 149-nucleotide region between –142 and +7 was essential for promoter activity in the NADH–GOGAT gene.

scholarly journals Quantitative Intercellular Localization of NADH-Dependent Glutamate Synthase Protein in Different Types of Root Cells in Rice Plants

1999 ◽  
Vol 119 (2) ◽  
pp. 409-416 ◽  
Author(s):  
Toshihiko Hayakawa ◽  
Laura Hopkins ◽  
Lucy J. Peat ◽  
Tomoyuki Yamaya ◽  
Alyson K. Tobin
Keyword(s):  
Glutamate Synthase ◽  
Root Cells ◽  
Rice Plants ◽  
Different Types

scholarly journals DIFFERENT NITRATE AND AMMONIUM LEVELS MEDIA ON CHANGES OF NITROGEN ASSIMILATION ENZYMES IN RICE

2021 ◽  
Vol 7 (1) ◽  
pp. 25-31
Author(s):  
Tri Handoyo ◽  
Laily Ilman Widuri ◽  
Didik Pudji Restanto
Keyword(s):  
Nitrogen Assimilation ◽  
Glutamate Synthase ◽  
Nitrogen Sources ◽  
Nitrate Content ◽  
Limiting Factor ◽  
Rice Plants ◽  
Glutamine Synthase ◽  
N Assimilation ◽  
Rice Yields ◽  
Different Sources

Nitrogen (N) is an important nutrient for the growth and development of rice plants, required in large quantity and often limiting factor of rice yields. The research was to understand the different sources and levels of nitrogen in rice plant on the activity of N assimilation enzymes, including nitrate reductase (NR), glutamine synthase (GS) content, glutamate synthase (Gogat) content, content, ammonium (NH4+) and nitrate (NO3-) content on the leaves. Paddy (Ciherang variety) was grown in sand media containing Hoagland solution with different sources (ammonium and nitrate) and levels (0.4, 0.8, 1.6, 3.2, 6.4, and 12.8 mM) of nitrogen. Nitrogen assimilation was observed from leaves at one month of age. The NR activity increased on both Nitrogen sources, it was a higher activity in media contained nitrate. Also, the activity of GS showed higher in media contains nitrate, but its activity was decreased after application 1.6 mM of nitrate and 3.2 mM of ammonium. Western blot analysis of GS1 and GS2 showed that the band pattern of protein was similar to these enzyme activities. Nitrate content in leaves gradually increased in both sources of nitrogen and higher than 3.2 mM ammonium application caused an increase in ammonium content in leaves, but the nitrate content decreased. This research resulted that the available source of N for rice was in nitrate form, easily by the rice plants during the growth stage.

Sign in / Sign up

Export Citation Format

Share Document