Genetic analysis of the obligate parasitic barley powdery mildew fungus based on RFLP and virulence loci

1990 ◽  
Vol 79 (5) ◽  
pp. 705-712 ◽  
Author(s):  
S. K. Christiansen ◽  
H. Giese
Keyword(s):  
Powdery Mildew ◽  
Genetic Analysis ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

Impact of proquinazid on appressorial development of the barley powdery mildew fungus Blumeria graminis f.sp hordei

2009 ◽  
Vol 94 (2-3) ◽  
pp. 127-132 ◽  
Author(s):  
Sally R. Gilbert ◽  
Hans J. Cools ◽  
Bart A. Fraaije ◽  
Andy M. Bailey ◽  
John A. Lucas
Keyword(s):  
Powdery Mildew ◽  
Blumeria Graminis ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals The isoelectric point of proteins influences their translocation to the extrahaustorial matrix of the barley powdery mildew fungus

2019 ◽  
Vol 21 (12) ◽  
Author(s):  
Lara Smigielski ◽  
Geziel B. Aguilar ◽  
Mark Kwaaitaal ◽  
Wen‐Jing Zhang ◽  
Hans Thordal‐Christensen
Keyword(s):  
Powdery Mildew ◽  
Isoelectric Point ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew ◽  
Extrahaustorial Matrix

Antifungal Activity of Zanthoxylium schinifolium Against Fusarium graminearum, a Barley Powdery Mildew Fungus.

2008 ◽  
Vol 18 (7) ◽  
pp. 974-979 ◽  
Author(s):  
Byum-Soo Kim ◽  
Han-Su Jang ◽  
Chung-Sig Choi ◽  
Jong-Sik Kim ◽  
Gi-Seok Kwon ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Powdery Mildew ◽  
Fusarium Graminearum ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals Host-Induced Gene Silencing in Barley Powdery Mildew Reveals a Class of Ribonuclease-Like Effectors

2013 ◽  
Vol 26 (6) ◽  
pp. 633-642 ◽  
Author(s):  
Clara Pliego ◽  
Daniela Nowara ◽  
Giulia Bonciani ◽  
Dana M. Gheorghe ◽  
Ruo Xu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Gene Silencing ◽  
Transcript Abundance ◽  
Powdery Mildew Fungus ◽  
Host Cell Death ◽  
Barley Powdery Mildew ◽  
Biotrophic Pathogens ◽  
Powdery Mildew Fungi ◽  
Bona Fide ◽  
Do So

Obligate biotrophic pathogens of plants must circumvent or counteract defenses to guarantee accommodation inside the host. To do so, they secrete a variety of effectors that regulate host immunity and facilitate the establishment of pathogen feeding structures called haustoria. The barley powdery mildew fungus Blumeria graminis f. sp. hordei produces a large number of proteins predicted to be secreted from haustoria. Fifty of these Blumeria effector candidates (BEC) were screened by host-induced gene silencing (HIGS), and eight were identified that contribute to infection. One shows similarity to β-1,3 glucosyltransferases, one to metallo-proteases, and two to microbial secreted ribonucleases; the remainder have no similarity to proteins of known function. Transcript abundance of all eight BEC increases dramatically in the early stages of infection and establishment of haustoria, consistent with a role in that process. Complementation analysis using silencing-insensitive synthetic cDNAs demonstrated that the ribonuclease-like BEC 1011 and 1054 are bona fide effectors that function within the plant cell. BEC1011 specifically interferes with pathogen-induced host cell death. Both are part of a gene superfamily unique to the powdery mildew fungi. Structural modeling was consistent, with BEC1054 adopting a ribonuclease-like fold, a scaffold not previously associated with effector function.

Genetic analysis of the avirulence of wheatgrass powdery mildew fungus on common wheat

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 913-917 ◽  
Author(s):  
Y. Tosa
Keyword(s):  
Triticum Aestivum ◽  
Powdery Mildew ◽  
Genetic Analysis ◽  
Common Wheat ◽  
Erysiphe Graminis ◽  
Wheat Cultivars ◽  
Avirulence Genes ◽  
Powdery Mildew Fungus ◽  
Major Genes ◽  
Formae Speciales

F1 hybrid cultures between Erysiphe graminis f.sp. agropyri (wheatgrass mildew fungus) and E. graminis f.sp. tritici (wheat mildew fungus) were produced by using a common host of the two formae spéciales. When three common wheat cultivars, Triticum aestivum cv. Norin 4, T. aestivum cv. Norin 10, and T. compactum cv. No. 44, were inoculated with a population of F1 cultures, avirulent and virulent cultures segregated in a 3:1 ratio. This indicated that two major genes are involved in the avirulence of E. graminis f.sp. agropyri, Ak-1, on each of the three cultivars. Further analyses revealed that the three pairs of avirulence genes have one gene in common. On T. aestivum cv. Shin-chunaga, T. aestivum cv. Norin 26, and a strain of T. macha, the F1 population segregated in the same pattern as on T. aestivum cv. Norin 4, indicating that the same pair of avirulence genes is operating on these four cultivars. On T. aestivum cv. Red Egyptian the distribution of F1 phenotypes was continuous, suggesting that no major genes are involved in the avirulence of Ak-1 on this cultivar.Key words: powdery mildew, Erysiphe graminis, avirulence, wheat, wheatgrass.

Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus

Planta ◽  
2005 ◽  
Vol 223 (5) ◽  
pp. 1010-1023 ◽  
Author(s):  
Kirsten A. Nielsen ◽  
Maria Hrmova ◽  
Janni Nyvang Nielsen ◽  
Karin Forslund ◽  
Stefan Ebert ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Powdery Mildew ◽  
Hordeum Vulgare L ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew

scholarly journals Barley ROP Binding Kinase1 Is Involved in Microtubule Organization and in Basal Penetration Resistance to the Barley Powdery Mildew Fungus

2012 ◽  
Vol 159 (1) ◽  
pp. 311-320 ◽  
Author(s):  
Christina Huesmann ◽  
Tina Reiner ◽  
Caroline Hoefle ◽  
Jutta Preuss ◽  
Manuela E. Jurca ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Penetration Resistance ◽  
Powdery Mildew Fungus ◽  
Microtubule Organization ◽  
Barley Powdery Mildew

Recombination of alleles conferring specific resistance to powdery mildew at the Mla locus in barley

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 460-468 ◽  
Author(s):  
Mamatha Mahadevappa ◽  
Richard A. DeScenzo ◽  
Roger P. Wise
Keyword(s):  
Powdery Mildew ◽  
Storage Proteins ◽  
Specific Resistance ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Genetic Distances ◽  
Hordeum Vulgare L ◽  
Powdery Mildew Fungus ◽  
Barley Powdery Mildew ◽  
Mla Locus

In barley (Hordeum vulgare L.), the Mla locus conditions reaction to the powdery mildew fungus Erysiphe graminis f.sp. hordei. Enrichment for genetic recombinants in the Mla region is possible by screening for recombination events between the flanking endosperm storage proteins hordeins C and B. Reciprocal crosses were made between the Franger (C.I. 16151) and Rupee (C.I. 16155) lines carrying the (Mla6 + Mla14) and Mla13 alleles, respectively. Recombinants were identified from F2 segregants by analyzing the extracted hordein polypeptides by sodium dodecyl sulphate – polyacrylamide gel electrophoresis. Two hundred and seventy-six recombinant gametes were identified from the 1800 seeds that were screened. Recombination of Mla alleles was analyzed by inoculating F4 recombinant lines with three isolates of E. graminis (A27, 5874, and CR3), which recognize specific Mla alleles. The linkage order established is Hor1–Mla6–Mla13–Mla14–Hor2. The genetic distances between Hor1–Mla6, Mla6–Mla13, and Mla13–Hor2, obtained using Mapmaker 3.0b F3 intercross analysis, are 3.9, 0.2, and 5.2 cM, respectively.Key words: recombinant, barley, powdery mildew, Mla, hordein.

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