Neuropeptide gene expression in brain is differentially regulated by midbrain dopamine neurons

1990 ◽  
Vol 80 (3) ◽  
Author(s):  
N. Lindefors ◽  
S. Bren� ◽  
M. Herrera-Marschitz ◽  
H. Persson
Keyword(s):  
Gene Expression ◽  
Dopamine Neurons ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons

scholarly journals Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda L. Brown ◽  
Trevor A. Day ◽  
Christopher V. Dayas ◽  
Doug W. Smith
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Dopamine Neuron ◽  
Dopamine Neurons ◽  
Acid Decarboxylase ◽  
Cell Groups ◽  
Midbrain Dopamine ◽  
High Degree ◽  
Midbrain Dopamine Neurons

The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells. However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample. Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity. The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold. For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively. Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes. Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples. Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups. This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.

Gene expression profiling of midbrain dopamine neurons upon gestational nicotine exposure

2016 ◽  
Vol 55 (3) ◽  
pp. 467-482 ◽  
Author(s):  
Pınar Kanlikilicer ◽  
Die Zhang ◽  
Andrei Dragomir ◽  
Yasemin M. Akay ◽  
Metin Akay
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Dopamine Neurons ◽  
Nicotine Exposure ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons ◽  
Gestational Nicotine

scholarly journals Classification of Midbrain Dopamine Neurons Using Single-Cell Gene Expression Profiling Approaches

2020 ◽  
Vol 43 (3) ◽  
pp. 155-169 ◽  
Author(s):  
Jean-Francois Poulin ◽  
Zachary Gaertner ◽  
Oscar Andrés Moreno-Ramos ◽  
Rajeshwar Awatramani
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Dopamine Neurons ◽  
Cell Gene Expression ◽  
Midbrain Dopamine ◽  
Cell Gene ◽  
Midbrain Dopamine Neurons

Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism

2005 ◽  
Vol 18 (1) ◽  
pp. 19-31 ◽  
Author(s):  
James G. Greene ◽  
Raymond Dingledine ◽  
J. Timothy Greenamyre
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Selective Vulnerability ◽  
Dopamine Neurons ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons

Molecular heterogeneity of midbrain dopamine neurons

2016 ◽  
Vol 4 (Suppl. 2) ◽  
pp. A2.1
Author(s):  
Siew-Lan Ang
Keyword(s):  
Dopamine Neurons ◽  
Molecular Heterogeneity ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons
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