Gene expression profiling of midbrain dopamine neurons upon gestational nicotine exposure

2016 ◽  
Vol 55 (3) ◽  
pp. 467-482 ◽  
Author(s):  
Pınar Kanlikilicer ◽  
Die Zhang ◽  
Andrei Dragomir ◽  
Yasemin M. Akay ◽  
Metin Akay
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Dopamine Neurons ◽  
Nicotine Exposure ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons ◽  
Gestational Nicotine

scholarly journals Classification of Midbrain Dopamine Neurons Using Single-Cell Gene Expression Profiling Approaches

2020 ◽  
Vol 43 (3) ◽  
pp. 155-169 ◽  
Author(s):  
Jean-Francois Poulin ◽  
Zachary Gaertner ◽  
Oscar Andrés Moreno-Ramos ◽  
Rajeshwar Awatramani
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Dopamine Neurons ◽  
Cell Gene Expression ◽  
Midbrain Dopamine ◽  
Cell Gene ◽  
Midbrain Dopamine Neurons

Gene expression profiling of rat midbrain dopamine neurons: implications for selective vulnerability in parkinsonism

2005 ◽  
Vol 18 (1) ◽  
pp. 19-31 ◽  
Author(s):  
James G. Greene ◽  
Raymond Dingledine ◽  
J. Timothy Greenamyre
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Selective Vulnerability ◽  
Dopamine Neurons ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons

scholarly journals Purity and Enrichment of Laser-Microdissected Midbrain Dopamine Neurons

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Amanda L. Brown ◽  
Trevor A. Day ◽  
Christopher V. Dayas ◽  
Doug W. Smith
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Dopamine Neuron ◽  
Dopamine Neurons ◽  
Acid Decarboxylase ◽  
Cell Groups ◽  
Midbrain Dopamine ◽  
High Degree ◽  
Midbrain Dopamine Neurons

The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for the study of gene expression in phenotypically identified cells. However, if the resultant gene expression profiles are to be accurately ascribed, it is necessary to determine the extent of contamination by nontarget cells in the microdissected sample. Here, we show that midbrain dopamine neurons can be laser-microdissected to a high degree of enrichment and purity. The average enrichment for tyrosine hydroxylase (TH) gene expression in the microdissected sample relative to midbrain sections was approximately 200-fold. For the dopamine transporter (DAT) and the vesicular monoamine transporter type 2 (Vmat2), average enrichments were approximately 100- and 60-fold, respectively. Glutamic acid decarboxylase (Gad65) expression, a marker for GABAergic neurons, was several hundredfold lower than dopamine neuron-specific genes. Glial cell and glutamatergic neuron gene expression were not detected in microdissected samples. Additionally, SN and VTA dopamine neurons had significantly different expression levels of dopamine neuron-specific genes, which likely reflects functional differences between the two cell groups. This study demonstrates that it is possible to laser-microdissect dopamine neurons to a high degree of cell purity. Therefore gene expression profiles can be precisely attributed to the targeted microdissected cells.

Neuropeptide gene expression in brain is differentially regulated by midbrain dopamine neurons

1990 ◽  
Vol 80 (3) ◽  
Author(s):  
N. Lindefors ◽  
S. Bren� ◽  
M. Herrera-Marschitz ◽  
H. Persson
Keyword(s):  
Gene Expression ◽  
Dopamine Neurons ◽  
Midbrain Dopamine ◽  
Midbrain Dopamine Neurons

scholarly journals Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology

Brain ◽  
2008 ◽  
Vol 132 (7) ◽  
pp. 1795-1809 ◽  
Author(s):  
Filip Simunovic ◽  
Ming Yi ◽  
Yulei Wang ◽  
Laurel Macey ◽  
Lauren T. Brown ◽  
...  
Keyword(s):  
Gene Expression ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Substantia Nigra ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Dopamine Neurons

236: Microarray analysis of the gene expression profiling in the cytotrophoblasts on nicotine exposure

2011 ◽  
Vol 204 (1) ◽  
pp. S101
Author(s):  
Hee young Cho ◽  
Hyewon Hur ◽  
Ga Hyun Son ◽  
Ha Yan Kwon ◽  
Yong Won Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Microarray Analysis ◽  
Expression Profiling ◽  
Nicotine Exposure

Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling

2002 ◽  
Vol 69 ◽  
pp. 135-142 ◽  
Author(s):  
Elena M. Comelli ◽  
Margarida Amado ◽  
Steven R. Head ◽  
James C. Paulson
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Affymetrix Genechip ◽  
Microarray Technology ◽  
Gene Arrays ◽  
Glycosyltransferase Gene

The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.

278: Gene Expression Profiling of Prostatic Epithelial Cells in Two-Dimensional Versus Three-Dimensional Cultures

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Toshiyuki Tsunoda ◽  
Junichi Inocuchi ◽  
Darren Tyson ◽  
Seiji Naito ◽  
David K. Ornstein
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Three Dimensional ◽  
Two Dimensional
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