Characterization of plasmid transformation in Bacillus subtilis: Kinetic properties and the effect of DNA conformation

1979 ◽  
Vol 167 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Sara Contente ◽  
David Dubnau
Keyword(s):  
Bacillus Subtilis ◽  
Dna Conformation ◽  
Kinetic Properties ◽  
Plasmid Transformation

Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts

1987 ◽  
Vol 146 (4) ◽  
pp. 353-357 ◽  
Author(s):  
T. Akamatsu ◽  
J. Sekiguchi
Keyword(s):  
Bacillus Subtilis ◽  
Plasmid Transformation

Structural Characterization of Bacillus subtilis Membrane Protein Bmr: An In Silico Approach

2015 ◽  
Vol 10 (3) ◽  
pp. 226-236
Author(s):  
Amit Nargotra ◽  
Rukmankesh ◽  
Shakir Ali ◽  
Surrinder Koul
Keyword(s):  
Bacillus Subtilis ◽  
Membrane Protein ◽  
Structural Characterization ◽  
In Silico

scholarly journals Sequence and characterization of Bacillus subtilis CheW.

1992 ◽  
Vol 267 (17) ◽  
pp. 12055-12060
Author(s):  
D.W. Hanlon ◽  
L.M. Márquez-Magaña ◽  
P.B. Carpenter ◽  
M.J. Chamberlin ◽  
G.W. Ordal
Keyword(s):  
Bacillus Subtilis

scholarly journals Regulation Reversal Mutation: Characterization of End Product-activated Mutants of Bacillus subtilis

1967 ◽  
Vol 242 (21) ◽  
pp. 4948-4955
Author(s):  
John H. Coats ◽  
Eugene W. Nester
Keyword(s):  
Bacillus Subtilis

scholarly journals Characterization of TseB: A new actor in cell wall elongation in Bacillus subtilis

2021 ◽  
Author(s):  
Jordan Delisle ◽  
Baptiste Cordier ◽  
Stéphane Audebert ◽  
Matthieu Pophillat ◽  
Caroline Cluzel ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis

scholarly journals Cloning and Characterization of a Chromosomal Class C β-Lactamase and Its Regulatory Gene in Laribacter hongkongensis

2005 ◽  
Vol 49 (5) ◽  
pp. 1957-1964 ◽  
Author(s):  
Susanna K. P. Lau ◽  
Pak-leung Ho ◽  
Maria W. S. Li ◽  
Hoi-wah Tsoi ◽  
Raymond W. H. Yung ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Southern Hybridization ◽  
Regulatory Gene ◽  
Kinetic Properties ◽  
Restriction Fragments ◽  
Regulatory Systems ◽  
Laribacter Hongkongensis ◽  
Consensus Motifs ◽  
Class C

ABSTRACT Laribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pI 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHK5 was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, bla LHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria.

scholarly journals Purification and characterization of Bacillus subtilis methyl-accepting chemotaxis protein methyltransferase II.

1982 ◽  
Vol 257 (14) ◽  
pp. 8412-8417 ◽  
Author(s):  
A Burgess-Cassler ◽  
A H Ullah ◽  
G W Ordal
Keyword(s):  
Bacillus Subtilis ◽  
Purification And Characterization ◽  
Protein Methyltransferase

scholarly journals Further Genetic and Enzymological Characterization of the Three Bacillus subtilis Deoxyribonucleic Acid Polymerases

1973 ◽  
Vol 248 (22) ◽  
pp. 7688-7700
Author(s):  
Kenneth B. Gass ◽  
Nicholas R. Cozzarelli
Keyword(s):  
Bacillus Subtilis ◽  
Deoxyribonucleic Acid

scholarly journals Some Kinetic Properties of Bacillus subtilis Glutamine Synthetase

1970 ◽  
Vol 245 (20) ◽  
pp. 5206-5213 ◽  
Author(s):  
Thomas F. Deuel ◽  
E.R. Stadtman
Keyword(s):  
Bacillus Subtilis ◽  
Glutamine Synthetase ◽  
Kinetic Properties

scholarly journals Characterization of Lysine Transfer Ribonucleic Acid from Vegetative Cells and Spores of Bacillus subtilis

1972 ◽  
Vol 247 (11) ◽  
pp. 3476-3484
Author(s):  
Ronald Y. Chuang ◽  
Roy H. Doi
Keyword(s):  
Bacillus Subtilis ◽  
Ribonucleic Acid ◽  
Vegetative Cells
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