DNA polymerase ? inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes

1984 ◽  
Vol 67 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Thomas W. Glover ◽  
Carol Berger ◽  
Jane Coyle ◽  
Barbara Echo
Keyword(s):  
Dna Polymerase ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Common Fragile Sites

Common fragile sites on human chromosomes represent transcriptionally active regions: evidence from camptothecin

1998 ◽  
Vol 102 (4) ◽  
pp. 409-414 ◽  
Author(s):  
I. Sbrana ◽  
Patrizia Zavattari ◽  
Roberto Barale ◽  
Antonio Musio
Keyword(s):  
Active Regions ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Common Fragile Sites

Family study of common fragile sites

1989 ◽  
Vol 82 (2) ◽  
pp. 191-193 ◽  
Author(s):  
Yoshitsugu Sugio ◽  
Yoshikazu Kuroki
Keyword(s):  
Family Study ◽  
Fragile Sites ◽  
Common Fragile Sites

A Novel Technique for Observing the Internal Ultrastructure of Human Chromosomes with Known Karyotype

2008 ◽  
Vol 14 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Mohammad Ghazizadeh ◽  
Yoshihiro Sasaki ◽  
Tatsuo Oguro ◽  
Shigeru Sato ◽  
Seiko Egawa ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Osmium Tetroxide ◽  
Human Lymphocytes ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Chromosome Research ◽  
Novel Technique ◽  
Transmission Electron ◽  
Comprehensive Information ◽  
Normal Human

Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. In this study we have used a method in which metaphase chromosome spreads were prepared on a flexible thermoplastic membrane (ACLAR) film. To assess chromosome identity, a diamidino-phenylindole staining and karyotying was first done using a conventional cytogenetic system. The chromosome spreads were then fixed with 1% osmium tetroxide, stained with freshly prepared 2% tannic acid, dehydrated, and flat-embedded in epoxy resin. The resin sheet was easily detachable and carried whole chromosome spreads. By this method, TEM observation of chromosomes from normal human lymphocytes allowed a thorough examination of the ultrastructure of centromeres, telomeres, fragile sites, and other chromosomal regions. Various ultrastructural patterns including thick electron dense boundaries, less dense internal regions, and extended chromatin loops at the periphery of the chromosomes were discernible. Application of the present method to chromosome research is expected to provide comprehensive information on the internal ultrastructure of different chromosomal regions in relation to function.

Sign in / Sign up

Export Citation Format

Share Document