In vivo analysis of chromatin following nystatin-mediated import of active enzymes into Saccharomyces cerevisiae

1994 ◽  
Vol 242 (1) ◽  
pp. 100-104 ◽  
Author(s):  
Sabrina Venditti ◽  
Giorgio Camilloni
Keyword(s):  
Saccharomyces Cerevisiae ◽  
In Vivo Analysis

scholarly journals In silico and in vivo analysis reveal a novel gene in Saccharomyces cerevisiae trehalose metabolism

BMC Genomics ◽  
2003 ◽  
Vol 4 (1) ◽  
Author(s):  
Joelma F De Mesquita ◽  
Anita D Panek ◽  
Pedro S de Araujo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
In Silico ◽  
Novel Gene ◽  
Trehalose Metabolism ◽  
In Vivo Analysis

Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron

1987 ◽  
Vol 7 (1) ◽  
pp. 225-230 ◽  
Author(s):  
L A Fouser ◽  
J D Friesen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Point Mutations ◽  
Mrna Splicing ◽  
Polypyrimidine Tract ◽  
Context Change ◽  
Sequence Context ◽  
Primary Transcript ◽  
In Vivo Analysis ◽  
Specific Sequences

Point mutations, deletions, and a sequence context change were introduced at positions 3' to the internal conserved TACTAAC sequence of the Saccharomyces cerevisiae actin intron. In vivo analysis of yeast mRNA splicing suggests that, in contrast to the importance of the polypyrimidine tract in metazoan introns, specific sequences in this region are not required for efficient excision of a yeast intron. However, a double point mutation near the 3' junction (GG/AC) does severely inhibit splicing. Although this mutagenesis of the 3' junction, as well as deletion of most nucleotides between the TACTAAC and the 3' junction, caused only a slight accumulation of primary transcript, the observed accumulation of lariat intermediate by these mutants demonstrates the significance of this region for a step(s) in the splicing process after lariat formation.

scholarly journals In vivo analysis of Saccharomyces cerevisiae plasma membrane ATPase Pma1p isoforms with increased in vitro H+/ATP stoichiometry

2012 ◽  
Vol 102 (2) ◽  
pp. 401-406 ◽  
Author(s):  
Stefan de Kok ◽  
Duygu Yilmaz ◽  
Jean-Marc Daran ◽  
Jack T. Pronk ◽  
Antonius J. A. van Maris
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Plasma Membrane Atpase ◽  
Membrane Atpase ◽  
In Vivo Analysis

scholarly journals In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae

2007 ◽  
Vol 26 (16) ◽  
pp. 3783-3793 ◽  
Author(s):  
John Mc Intyre ◽  
Eric G D Muller ◽  
Stefan Weitzer ◽  
Brian E Snydsman ◽  
Trisha N Davis ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Budding Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
In Vivo Analysis

scholarly journals In Vivo Analysis of Folate Coenzymes and Their Compartmentation in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (2) ◽  
pp. 371-381 ◽  
Author(s):  
J Bryan McNeil ◽  
Andrew L Bognar ◽  
Ronald E Pearlman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Serine Hydroxymethyltransferase ◽  
Mitochondrial Enzymes ◽  
Glycine Cleavage System ◽  
In Vivo Analysis ◽  
Cytoplasmic Compartment ◽  
Glycine Cleavage ◽  
In Vivo Growth ◽  
Glycine Synthesis

Abstract In eukaryotes, enzymes responsible for the interconversion of one-carbon units exist in parallel in both mitochondria and the cytoplasm. Strains of Saccharomyces cerevisiae were constructed that possess combinations of gene disruptions at the SHM1 [mitochondrial serine hydroxymethyltransferase (SHMTm)], SHM2 [cytoplasmic SHMT (SHMTc)], MIS1 [mitochondrial C1-tetrahydrofolate synthase (C1-THFSm)], ADE3 [cytoplasmic C1-THF synthase (C1-THFSc)], GCV1 [glycine cleavage system (GCV) protein T], and the GLY1 (involved in glycine synthesis) loci. Analysis of the in vivo growth characteristics and phenotypes was used to determine the contribution to cytoplasmic nucleic acid and amino acid anabolism by the mitochondrial enzymes involved in the interconversion of folate coenzymes. The data indicate that mitochondria transport formate to the cytoplasmic compartment and mitochondrial synthesis of formate appears to rely primarily on SHMTm rather than the glycine cleavage system. The glycine cleavage system and SHMTm cooperate to specifically synthesize serine. With the inactivation of SHM1, however, the glycine cleavage system can make an observable contribution to the level of mitochondrial formate. Inactivation of SHM1, SHM2 and ADE3 is required to render yeast auxotrophic for TMP and methionine, suggesting that TMP synthesized in mitochondria may be available to the cytoplasmic compartment.

scholarly journals Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.

1987 ◽  
Vol 7 (1) ◽  
pp. 225-230 ◽  
Author(s):  
L A Fouser ◽  
J D Friesen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Point Mutations ◽  
Mrna Splicing ◽  
Polypyrimidine Tract ◽  
Context Change ◽  
Sequence Context ◽  
Primary Transcript ◽  
In Vivo Analysis ◽  
Specific Sequences

Point mutations, deletions, and a sequence context change were introduced at positions 3' to the internal conserved TACTAAC sequence of the Saccharomyces cerevisiae actin intron. In vivo analysis of yeast mRNA splicing suggests that, in contrast to the importance of the polypyrimidine tract in metazoan introns, specific sequences in this region are not required for efficient excision of a yeast intron. However, a double point mutation near the 3' junction (GG/AC) does severely inhibit splicing. Although this mutagenesis of the 3' junction, as well as deletion of most nucleotides between the TACTAAC and the 3' junction, caused only a slight accumulation of primary transcript, the observed accumulation of lariat intermediate by these mutants demonstrates the significance of this region for a step(s) in the splicing process after lariat formation.

scholarly journals In Vivo Analysis of Chromosome Condensation in Saccharomyces cerevisiae

2007 ◽  
Vol 18 (2) ◽  
pp. 557-568 ◽  
Author(s):  
Amit C.J. Vas ◽  
Catherine A. Andrews ◽  
Kathryn Kirkland Matesky ◽  
Duncan J. Clarke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Topoisomerase Ii ◽  
Spindle Assembly Checkpoint ◽  
Cell Cycle Control ◽  
Transient State ◽  
Chromosome Condensation ◽  
Lac Repressor ◽  
Yeast Saccharomyces Cerevisiae ◽  
In Vivo Analysis

Although chromosome condensation in the yeast Saccharomyces cerevisiae has been widely studied, visualization of this process in vivo has not been achieved. Using Lac operator sequences integrated at two loci on the right arm of chromosome IV and a Lac repressor-GFP fusion protein, we were able to visualize linear condensation of this chromosome arm during G2/M phase. As previously determined in fixed cells, condensation in yeast required the condensin complex. Not seen after fixation of cells, we found that topoisomerase II is required for linear condensation. Further analysis of perturbed mitoses unexpectedly revealed that condensation is a transient state that occurs before anaphase in budding yeast. Blocking anaphase progression by activation of the spindle assembly checkpoint caused a loss of condensation that was dependent on Mad2, followed by a delayed loss of cohesion between sister chromatids. Release of cells from spindle checkpoint arrest resulted in recondensation before anaphase onset. The loss of condensation in preanaphase-arrested cells was abrogated by overproduction of the aurora B kinase, Ipl1, whereas in ipl1-321 mutant cells condensation was prematurely lost in anaphase/telophase. In vivo analysis of chromosome condensation has therefore revealed unsuspected relationships between higher order chromatin structure and cell cycle control.

In vivo analysis of the acidic ribosomal proteins BmP1 and BmP2 of the silkworm Bombyx mori in the yeast Saccharomyces cerevisiae

Gene ◽  
2007 ◽  
Vol 388 (1-2) ◽  
pp. 27-33 ◽  
Author(s):  
Petrina Koumarianou ◽  
Alberto Garcia Marcos ◽  
Juan P.G. Ballesta ◽  
Sophia Kouyanou-Koutsoukou
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Bombyx Mori ◽  
Ribosomal Proteins ◽  
Yeast Saccharomyces Cerevisiae ◽  
In Vivo Analysis ◽  
Silkworm Bombyx Mori
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