Electron microscopic demonstration of acid phosphatase activity in the developing and mature heterophils of the chicken

1973 ◽  
Vol 37 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Jan L. E. Ericsson ◽  
M. Krishnan Nair
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Electron Microscopic ◽  
Microscopic Demonstration ◽  
Electron Microscopic Demonstration

scholarly journals CYTOLOGICAL STUDIES ON TWO FUNCTIONAL HEPATOMAS

1962 ◽  
Vol 15 (2) ◽  
pp. 289-312 ◽  
Author(s):  
Edward Essner ◽  
Alex B. Novikoff
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Golgi Apparatus ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Secretory Granules ◽  
Dynamic State ◽  
Secretory Product ◽  
Electron Microscopic ◽  
Golgi Membranes

The Reuber hepatoma H-35 and Morris hepatoma 5123 have been studied by electron microscopy and by cytochemical staining methods for a number of phosphatases. These studies emphasize the resemblances of the two tumors to rat liver, but they also indicate distinctive features in each of the three tissues. Secretory product accumulates within the cisternae of the Golgi apparatus that dilate to form the Golgi vacuoles. The vacuoles apparently separate, and secretory material undergoes further condensation within them. These "secretory vacuoles" possess acid phosphatase activity and may thus be considered lysosomes. The membranes of the Golgi apparatus are without acid phosphatase activity but show high levels of thiaminepyrophosphatase activity. The endoplasmic reticulum also hydrolyzes thiaminepyrophosphate but at a lower rate; it hydrolyzes the diphosphates of uridine, guanosine, and inosine rapidly. These observations and the electron microscopic images are consistent with the view that the cytomembranes are in a dynamic state of flux, movement, and transformation in the living cell, and that smooth surfaced derivatives of the endoplasmic reticulum become refashioned into the Golgi membranes as the Golgi membranes are being refashioned into those that delimit secretory vacuoles. The variations encountered in the two hepatomas are described. The electron microscope literature dealing with the relations of the Golgi apparatus to secretory granules, on the one hand, and the endoplasmic reticulum, on the other, is reviewed briefly.

scholarly journals Electron microscopic cytochemical studies on acid phosphatase activity in acute myocardial ischemia.

1983 ◽  
Vol 24 (4) ◽  
pp. 595-606 ◽  
Author(s):  
Naomi NAKAMURA ◽  
Yasufumi SASAI ◽  
Youichi TAKEYAMA ◽  
Takashi KATAGIRI
Keyword(s):  
Acid Phosphatase ◽  
Myocardial Ischemia ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Acute Myocardial Ischemia ◽  
Electron Microscopic
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