MENINGOENCEPHALITIS IN BROILER CHICKS DUE TO ASPERGILLUS FUMIGATUS INFECTION

Forty five cases, 7-14 day-old chicks were studied, they showed respiratory and nervous signs with yellowish granulomatous necrotic foci on the air sacs, liver, kidneys, cerebellum and cerebrum. Swabs were taken 7 days post inoculation, culture was made on Sabouraud's dextros agar,. Aspergillus fumigatus was isolated. Diagnosis was confirmed by experimentally exposed one day-old chicks to aerosols of A. fumigatus conidia and also by microscopic demonstration of typical fruiting bodies and spores of the fungus in fresh preparations made from Sabouraud's agar. tissues from fungal-infected Examination of histological sections of cerebrum and cerebellum chicks stained with hematoxylin and eosin and PAS stains revealed granulomas lesion with a central area of necrosis containing heterophils surrounded by macrophages, gaint cells, lymphocytes and layer of fibrous tissue. It was concluded that the cases deal within this study had been infected with A. fumigataus.

Studies on microscopic technique and culture on Sabouraud's dextrose agar medium for diagnosis of dermatophytes infection

Dermatophytoses (a fungal infection of the skin, hair and nail, usually caused by dermatophytes) constitutes an important public health problem because of its high prevalence and associated morbidity but not life-threatening. Three genera of dermatophytes are recognized based on the site and pattern of fungal invasion. Dermatophytes are the predominant pathogenic mould, but yeasts (especially Candida albicans) and non-dermatophytic moulds may also be implicated. For accurate diagnosis of dermatophytoses requires microscopic demonstration and isolation and identification by culture. This study evaluates the usefulness of microscopic technique and culture for the isolation and identification of dermatophytes from clinical samples. Thirty samples were included in this study for detection of fungal elements by both methods but sensitivity of microscopic demonstration and culture were 60.0% and 66.7% respectively. As the sensitivity of microscopic demonstration (60.0%) is almost equal to the isolation and identification rate (66.7%), requires further evaluation in large scale as its ready to use format makes the application and microscopy much easier and faster.DOI: http://dx.doi.org/10.3329/kyamcj.v3i1.13658 KYAMC Journal Vol. 3, No.-1, June 2012 pp.235-238