The role of calcium in in vitro release of eosinophil chemotactic factor of anaphylaxis (ECF-A) from guinea pig skin

1981 ◽  
Vol 270 (3) ◽  
pp. 371-374
Author(s):  
S. Yamamoto ◽  
I. Kimura ◽  
K. Takagaki ◽  
T. Yamura
Keyword(s):  
Guinea Pig ◽  
In Vitro Release ◽  
Chemotactic Factor ◽  
Pig Skin ◽  
Vitro Release ◽  
Eosinophil Chemotactic Factor

In vitro Release of Eosinophil Chemotactic Factor of Anaphylaxis (ECF-A) from Guinea Pig Skin

1979 ◽  
Vol 60 (4) ◽  
pp. 465-468 ◽  
Author(s):  
Isamu Kimura ◽  
Shoso Yamamoto ◽  
Takuso Yamura
Keyword(s):  
Guinea Pig ◽  
Chemotactic Factor ◽  
Pig Skin ◽  
Eosinophil Chemotactic Factor

Specificity of mammalian kallidinogen

1964 ◽  
Vol 207 (4) ◽  
pp. 901-905 ◽  
Author(s):  
J. C. Fasciolo ◽  
K. Halvorsen
Keyword(s):  
Blood Pressure ◽  
Pressure Drop ◽  
Guinea Pig ◽  
Human Plasma ◽  
In Vitro Release ◽  
Rat Plasma ◽  
Vitro Release

Hog pancreas kallikrein does not cause a blood pressure drop in the rat, but lowers the blood pressure in the dog. Since this lack of effect could be due to the inability of hog pancreas kallikrein to act upon rat plasma, the in vitro release of kallidin with the plasma of different species was studied. Human, dog, swine, rat, guinea pig, and ox plasmas were incubated for different periods of time with hog pancreas kallikrein, and the bradykininogen (kallidinogen) consumed and the kallidin released were measured. It was found that hog pancreas kallikrein does not release any kallidin with rat and guinea pig plasma; it releases small amounts with pig plasma and greater amounts with bovine, dog, and human plasma. The bradykininogen consumption showed the same sequence.

scholarly journals Nitric oxide synthase acutely regulates progesterone production by in vitro cultured rabbit corpora lutea

1999 ◽  
Vol 160 (2) ◽  
pp. 275-283 ◽  
Author(s):  
A Gobbetti ◽  
C Boiti ◽  
C Canali ◽  
M Zerani
Keyword(s):  
Nitric Oxide ◽  
In Vitro Release ◽  
Inhibitory Effect ◽  
Corpora Lutea ◽  
No Donor ◽  
Nos Inhibitor ◽  
Oxide Synthase ◽  
Vitro Release

We examined the presence and the regulation of nitric oxide (NO) synthase (NOS) using in vitro cultured corpora lutea (CL) obtained from rabbits at days 4 and 9 of pseudopregnancy. The role of NO and NOS on steroidogenesis was also investigated using the same CL preparations after short-term incubations (30 min and 2 h) with the NO donor, sodium nitroprusside (NP), the NOS inhibitor, Nomega-nitro-l-arginine methyl ester (l-NAME) and prostaglandin (PG) F-2alpha. The basal NOS activity was greater in CL at day 4 than at day 9, and was also differently modulated by PGF-2alpha, depending on the age of the CL. The addition of PGF-2alpha to day 4 CL had no effect, but PGF-2alpha on day 9 caused a threefold increase in NOS activity. NP caused a two- to fivefold decrease in release of progesterone from CL of both ages, and this inhibitory effect on steroidogenesis was reversed by l-NAME. All treatments failed to modify basal androgens and 17beta-oestradiol was not detectable in either control or treated CL. These results suggest that NO is effectively involved in the regulation process of steroidogenesis, independently of 17beta-oestradiol. PGF-2alpha had no effect on day 4, but induced luteolysis on day 9, by reducing progesterone (P</=0. 01) to about 18% of control. The luteolytic action of PGF-2alpha was completely reversed by co-incubation with l-NAME, thus supporting the hypothesis that luteolysis is mediated by NO. The addition of NP or l-NAME did not modify the in vitro release of PGF-2alpha. We hypothesised that PGF-2alpha upregulates NOS activity and, consequently, the production of NO, which acutely inhibits progesterone release from day 9 CL of pseudopregnant rabbits.

In vitro release of endothelium-derived relaxing factor by acetylcholine is increased during the guinea pig pregnancy

1989 ◽  
Vol 161 (6) ◽  
pp. 1599-1605 ◽  
Author(s):  
Carl Weiner ◽  
Ernest Martinez ◽  
Liu Kang Zhu ◽  
Abdi Ghodsi ◽  
David Chestnut
Keyword(s):  
Guinea Pig ◽  
In Vitro Release ◽  
Relaxing Factor ◽  
Endothelium Derived Relaxing Factor ◽  
Vitro Release

scholarly journals Effect of Neutrophil-derived Eosinophil Chemotactic Factor (ECF) in Human and Guinea Pig Skin

1980 ◽  
Vol 74 (2) ◽  
pp. 109-111 ◽  
Author(s):  
Bean M. Czarnetzki ◽  
Peter J. Frosch ◽  
Fereydoun Vakilzadeh ◽  
Wolfgang B. Panneck
Keyword(s):  
Guinea Pig ◽  
Chemotactic Factor ◽  
Pig Skin ◽  
Eosinophil Chemotactic Factor

Role of in vitro release models in formulation development and quality control of parenteral depots

2009 ◽  
Vol 6 (12) ◽  
pp. 1283-1295 ◽  
Author(s):  
Claus Larsen ◽  
Susan Weng Larsen ◽  
Henrik Jensen ◽  
Anan Yaghmur ◽  
Jesper Østergaard
Keyword(s):  
Quality Control ◽  
In Vitro Release ◽  
Formulation Development ◽  
Vitro Release

Platelet-poor plasma serotonin in normal and preeclamptic pregnancy

1993 ◽  
Vol 39 (8) ◽  
pp. 1675-1678 ◽  
Author(s):  
C M Middelkoop ◽  
G A Dekker ◽  
A A Kraayenbrink ◽  
C Popp-Snijders
Keyword(s):  
Pregnant Women ◽  
Electrochemical Detection ◽  
In Vitro Release ◽  
Mean Values ◽  
Platelet Poor Plasma ◽  
Concentration Step ◽  
Plasma Serotonin ◽  
Vitro Release

Abstract We determined serotonin concentrations in platelet-poor plasma (PPP) from 11 preeclamptic pregnant women and 11 normal pregnant women by HPLC with electrochemical detection after a concentration step. Serotonin concentrations in PPP are very low in comparison with those in platelets, so it is very important to avoid in vitro release of serotonin from platelets. We therefore investigated three types of anticoagulants, the method of blood sampling, and the influence of whether the first or second 10 mL of blood is assayed. The type of anticoagulant proved to be important, K3-EDTA giving the best results. The serotonin concentrations in PPP from the preeclamptic pregnant women were significantly higher (P = 0.0001) than in the normal pregnant women: mean values +/- SD were 27.0 +/- 13.5 and 2.8 +/- 1.4 nmol/L, respectively. We suggest that the measurement of serotonin in PPP might be helpful in study of the role of serotonin in the development of preeclampsia.

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